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Taxonomic group: fungi / Basidiomycota (Phylum: Basidiomycota)
Organ / tissue: mycelium

The structure was elucidated in this paper
NCBI PubMed ID: 36596100
Publication DOI: 10.1016/j.foodres.2022.112146
Journal NLM ID: 9210143
Publisher: Ottawa, Ontario, Canada: CIFST, Elsevier Applied Science
Correspondence: Z. Zhu <zhyuanzhu

tust.edu.cn>
Institutions: State Key Laboratory of Food Nutrition and Safety, Tianjin University of Science and Technology, Tianjin 300457, PR China, College of Food Science and Engineering, Tianjin University of Science and Technology, Tianjin 300457, PR China, Laboratory of Food Nutrition and Safety, Ministry of Education, Tianjin Key Laboratory of Food Nutrition and Safety, Tianjin University of Science and Technology, Tianjin 300457, PR China, College of Public Health, Zunyi Medical University, Guizhou 563006, PR China

Pleurotus ostreatus is one of the most common edible and medicinal fungi in life, and its polysaccharide has been a hot research topic in recent years. In this paper, a new intracellular polysaccharide component named P. ostreatus polysaccharide (POP-W) was obtained from the mycelium of P. ostreatus, and its structure was analyzed. The results showed that its molecular weight was Mw = 3.034 × 103 kDa, and it did not contain protein and nucleic acid. POP-W was composed of mannose, glucose, galactose and xylose in a molar ratio of 40.34:47.60:7.97:4.09. The backbone of POP-W was α-D-Glcp(1→,→3,4)-α-D-Glcp(1→, →3,4)-α-D-Manp(1→,→3)-α -D-Galp(1→, →4)-α-D-Glcp(1→, →3)-α-D-Glcp(1→, →2)-β-D-Manp(1→, →4)-β-D-Xylp(1→. SEM and TGA analysis showed the structure of POP-W and good thermal stability. In addition, POP-W showed significant antioxidant activity in vitro. More importantly, POP-W protected PC12 cells induced by H2O2 by inhibiting the contents of lactate dehydrogenase (LDH) and malondialdehyde (MDA) and increasing the levels of superoxide dismutase (SOD) and reduced glutathione (GSH). Western blot detection of Caspase-3, BAX, Bcl-2, PI3K/Akt protein expression. The results showed that POP-W inhibited the expression of caspase-3 and BAX, while promoting the expression of Bcl-2. In addition, POP-W can also promote the phosphorylation of Akt. In conclusion, POP-W pretreatment can protect PC12 cells from H2O2-induced oxidative damage through PI3K/Akt signaling pathway and regulation of apoptosis-related pathway proteins. It provided a theoretical basis for the practical application of the polysaccharide of P. ostreatus in production.

polysaccharide, Structural characterization, Antioxidant, PC12 cells, P.ostreatus

Structure type: structural motif or average structure ; 3034
Location inside paper: table 2, Fig. 4(g), POP-W
Trivial name: polysaccharide (POP-W)
Compound class: IPS

Methods: 13C NMR, 1H NMR, methylation, GC-MS, Smith degradation, Western blotting, FTIR, composition analysis, HPLC, extraction, periodate oxidation, statistical analysis, antioxidant activities, cell viability assay, radical scavenging assay, SEM, TGA, isolation, oxidative stress, SOD, GSH, MDA
Comments, role: NMR temperature was not specified; published erroneous NMR chemical shifts of C2 aDGalp (76.05) was removed by CSDB staff.

NCBI Taxonomy refs (TaxIDs): 5322
Show glycosyltransferases

NMR conditions: in D2O [as TSV]

13C NMR data:
Linkage	Residue	C1	C2	C3	C4	C5	C6
4,4,4,4,3,3,4	aDGlcp	100.17	72.45	74.13	76.84	73.20	61.18
4,4,4,4,3	aDGalp	101.79	?	81.52	69.27	75.26	61.30
4,4,4,3,2	aDGlcp	100.10	70.93	75.53	70.91	73.89	60.99
4,4,4,3	aDManp	99.30	78.65	71.72	65.49	69.68	60.46
4,4,4	aDManp	101.25	71.59	81.29	76.41	72.86	62.73
4,4,3,3	aDGlcp	100.10	70.93	75.53	70.91	73.89	60.99
4,4,3	aDManp	102.12	69.54	77.94	70.84	73.14	60.89
4,4	aDManp	101.25	71.59	81.29	76.41	72.86	62.73
4,4,4,4,3,3,4,4,3,3,3	aDGlcp	100.10	70.93	75.53	70.91	73.89	60.99
4,4,4,4,3,3,4,4,3,3	bDGlcp
4,4,4,4,3,3,4,4,3	bDGlcp	101.64	71.62	82.20	69.58	75.46	62.19
4,4,4,4,3,3,4,4	bDGlcp
4,4,4,4,3,3	bDXylp	102.37	74.43	74.48	76.80	63.57
4,4,4,4	aDGalp	101.79	?	81.52	69.27	75.26	61.30
4,3,3	aDGlcp	100.10	70.93	75.53	70.91	73.89	60.99
4,3	aDManp	102.12	69.54	77.94	70.84	73.14	60.89
4	aDManp	101.25	71.59	81.29	76.41	72.86	62.73
	SUG


1H NMR data:
Linkage	Residue	H1	H2	H3	H4	H5	H6
4,4,4,4,3,3,4	aDGlcp	4.94	3.37	3.48	3.56	3.51	3.63
4,4,4,4,3	aDGalp	4.99	3.84	4.02	3.96	3.52	3.73
4,4,4,3,2	aDGlcp	4.89	3.66	3.72	3.43	3.82	3.74
4,4,4,3	aDManp	5.29	3.75	3.84	3.69	3.96	3.69
4,4,4	aDManp	5.19	3.62	3.92	3.82	3.76	3.74
4,4,3,3	aDGlcp	4.89	3.66	3.72	3.43	3.82	3.74
4,4,3	aDManp	5.03	4.22	3.95	3.74	3.71	3.75
4,4	aDManp	5.19	3.62	3.92	3.82	3.76	3.74
4,4,4,4,3,3,4,4,3,3,3	aDGlcp	4.89	3.66	3.72	3.43	3.82	3.74
4,4,4,4,3,3,4,4,3,3	bDGlcp
4,4,4,4,3,3,4,4,3	bDGlcp	4.57	3.61	3.71	4.02	3.65	3.80
4,4,4,4,3,3,4,4	bDGlcp
4,4,4,4,3,3	bDXylp	4.43	3.15	3.40	3.58	3.20
4,4,4,4	aDGalp	4.99	3.84	4.02	3.96	3.52	3.73
4,3,3	aDGlcp	4.89	3.66	3.72	3.43	3.82	3.74
4,3	aDManp	5.03	4.22	3.95	3.74	3.71	3.75
4	aDManp	5.19	3.62	3.92	3.82	3.76	3.74
	SUG


1H/13C HSQC data:
Linkage	Residue	C1/H1	C2/H2	C3/H3	C4/H4	C5/H5	C6/H6
4,4,4,4,3,3,4	aDGlcp	100.17/4.94	72.45/3.37	74.13/3.48	76.84/3.56	73.20/3.51	61.18/3.63
4,4,4,4,3	aDGalp	101.79/4.99	?/3.84	81.52/4.02	69.27/3.96	75.26/3.52	61.30/3.73
4,4,4,3,2	aDGlcp	100.10/4.89	70.93/3.66	75.53/3.72	70.91/3.43	73.89/3.82	60.99/3.74
4,4,4,3	aDManp	99.30/5.29	78.65/3.75	71.72/3.84	65.49/3.69	69.68/3.96	60.46/3.69
4,4,4	aDManp	101.25/5.19	71.59/3.62	81.29/3.92	76.41/3.82	72.86/3.76	62.73/3.74
4,4,3,3	aDGlcp	100.10/4.89	70.93/3.66	75.53/3.72	70.91/3.43	73.89/3.82	60.99/3.74
4,4,3	aDManp	102.12/5.03	69.54/4.22	77.94/3.95	70.84/3.74	73.14/3.71	60.89/3.75
4,4	aDManp	101.25/5.19	71.59/3.62	81.29/3.92	76.41/3.82	72.86/3.76	62.73/3.74
4,4,4,4,3,3,4,4,3,3,3	aDGlcp	100.10/4.89	70.93/3.66	75.53/3.72	70.91/3.43	73.89/3.82	60.99/3.74
4,4,4,4,3,3,4,4,3,3	bDGlcp
4,4,4,4,3,3,4,4,3	bDGlcp	101.64/4.57	71.62/3.61	82.20/3.71	69.58/4.02	75.46/3.65	62.19/3.80
4,4,4,4,3,3,4,4	bDGlcp
4,4,4,4,3,3	bDXylp	102.37/4.43	74.43/3.15	74.48/3.40	76.80/3.58	63.57/3.20
4,4,4,4	aDGalp	101.79/4.99	?/3.84	81.52/4.02	69.27/3.96	75.26/3.52	61.30/3.73
4,3,3	aDGlcp	100.10/4.89	70.93/3.66	75.53/3.72	70.91/3.43	73.89/3.82	60.99/3.74
4,3	aDManp	102.12/5.03	69.54/4.22	77.94/3.95	70.84/3.74	73.14/3.71	60.89/3.75
4	aDManp	101.25/5.19	71.59/3.62	81.29/3.92	76.41/3.82	72.86/3.76	62.73/3.74
	SUG

¹H NMR data:

Linkage	Residue	H1	H2	H3	H4	H5	H6
4,4,4,4,3,3,4	aDGlcp	4.94	3.37	3.48	3.56	3.51	3.63
4,4,4,4,3	aDGalp	4.99	3.84	4.02	3.96	3.52	3.73
4,4,4,3,2	aDGlcp	4.89	3.66	3.72	3.43	3.82	3.74
4,4,4,3	aDManp	5.29	3.75	3.84	3.69	3.96	3.69
4,4,4	aDManp	5.19	3.62	3.92	3.82	3.76	3.74
4,4,3,3	aDGlcp	4.89	3.66	3.72	3.43	3.82	3.74
4,4,3	aDManp	5.03	4.22	3.95	3.74	3.71	3.75
4,4	aDManp	5.19	3.62	3.92	3.82	3.76	3.74
4,4,4,4,3,3,4,4,3,3,3	aDGlcp	4.89	3.66	3.72	3.43	3.82	3.74
4,4,4,4,3,3,4,4,3,3	bDGlcp
4,4,4,4,3,3,4,4,3	bDGlcp	4.57	3.61	3.71	4.02	3.65	3.80
4,4,4,4,3,3,4,4	bDGlcp
4,4,4,4,3,3	bDXylp	4.43	3.15	3.40	3.58	3.20
4,4,4,4	aDGalp	4.99	3.84	4.02	3.96	3.52	3.73
4,3,3	aDGlcp	4.89	3.66	3.72	3.43	3.82	3.74
4,3	aDManp	5.03	4.22	3.95	3.74	3.71	3.75
4	aDManp	5.19	3.62	3.92	3.82	3.76	3.74
	SUG

¹³C NMR data:

Linkage	Residue	C1	C2	C3	C4	C5	C6
4,4,4,4,3,3,4	aDGlcp	100.17	72.45	74.13	76.84	73.20	61.18
4,4,4,4,3	aDGalp	101.79	?	81.52	69.27	75.26	61.30
4,4,4,3,2	aDGlcp	100.10	70.93	75.53	70.91	73.89	60.99
4,4,4,3	aDManp	99.30	78.65	71.72	65.49	69.68	60.46
4,4,4	aDManp	101.25	71.59	81.29	76.41	72.86	62.73
4,4,3,3	aDGlcp	100.10	70.93	75.53	70.91	73.89	60.99
4,4,3	aDManp	102.12	69.54	77.94	70.84	73.14	60.89
4,4	aDManp	101.25	71.59	81.29	76.41	72.86	62.73
4,4,4,4,3,3,4,4,3,3,3	aDGlcp	100.10	70.93	75.53	70.91	73.89	60.99
4,4,4,4,3,3,4,4,3,3	bDGlcp
4,4,4,4,3,3,4,4,3	bDGlcp	101.64	71.62	82.20	69.58	75.46	62.19
4,4,4,4,3,3,4,4	bDGlcp
4,4,4,4,3,3	bDXylp	102.37	74.43	74.48	76.80	63.57
4,4,4,4	aDGalp	101.79	?	81.52	69.27	75.26	61.30
4,3,3	aDGlcp	100.10	70.93	75.53	70.91	73.89	60.99
4,3	aDManp	102.12	69.54	77.94	70.84	73.14	60.89
4	aDManp	101.25	71.59	81.29	76.41	72.86	62.73
	SUG

The spectrum also has 2 signals at unknown positions (not plotted).

SMILES errors: aDGlcp(1-3)bDGlcp(1-3)bDGlcp(1-3)bDGlcp(1-4)/aDGlcp(1-4)/n=8/bDXylp(1-3)/aDGalp(1-3)/n=3/aDGalp(1-4)/[aDGlcp(1-2)aDManp(1-3)]aDManp(1-4)/n=2//[aDGlcp(1-3)aDManp(1-3)]aDManp(1-4)/n=4/[aDGlcp(1-3)aDManp(1-3)]aDManp(1-4)SUG:
SMILES error: could not calculate brutto descriptors of a molecule from SMILES [1*]O[C@H]1O[C@H](CO)[C@@H](O[C@H]2O[C@H](CO)[C@@H](O[C@H]3O[C@H](CO)[C@@H](O[C@H]4O[C@H](CO)[C@@H](O[C@H]5O[C@H](CO)[C@@H](O[C@H]6O[C@H](CO)[C@@H](O[C@H]7O[C@H](CO)[C@@H](O[C@H]8O[C@H](CO)[C@H](O)[C@H](O[C@H]9O[C@H](CO)[C@H](O)[C@H](O[C@H]%10O[C@H](CO)[C@H](O)[C@H](O[C@H]%11O[C@H](CO)[C@H](O)[C@H](O[C@@H]%12OC[C@@H](O[C@H]%13O[C@H](CO)[C@@H](O[C@H]%14O[C@H](CO)[C@@H](O[C@H]%15O[C@H](CO)[C@@H](O[C@H]%16O[C@H](CO)[C@@H](O[C@H]%17O[C@H](CO)[C@@H](O[C@@H]%18O[C@H](CO)[C@@H](O)[C@H](O[C@@H]%19O[C@H](CO)[C@@H](O)[C@H](O[C@@H]%20O[C@H](CO)[C@@H](O)[C@H](O[C@H]%21O[C@H](CO)[C@@H](O)[C@H](O)[C@H]%21O)[C@H]%20O)[C@H]%19O)[C@H]%18O)[C@H](O)[C@H]%17O)[C@H](O)[C@H]%16O)[C@H](O)[C@H]%15O)[C@H](O)[C@H]%14O)[C@H](O)[C@H]%13O)[C@H](O)[C@H]%12O)[C@H]%11O)[C@H]%10O)[C@H]9O)[C@H]8O)[C@H](O[C@H]8O[C@H](CO)[C@@H](O)[C@H](O)[C@@H]8O[C@H]8O[C@H](CO)[C@@H](O)[C@H](O)[C@H]8O)[C@@H]7O)[C@H](O[C@H]7O[C@H](CO)[C@@H](O)[C@H](O)[C@@H]7O[C@H]7O[C@H](CO)[C@@H](O)[C@H](O)[C@H]7O)[C@@H]6O)[C@H](O[C@H]6O[C@H](CO)[C@@H](O)[C@H](O[C@H]7O[C@H](CO)[C@@H](O)[C@H](O)[C@H]7O)[C@@H]6O)[C@@H]5O)[C@H](O[C@H]5O[C@H](CO)[C@@H](O)[C@H](O[C@H]6O[C@H](CO)[C@@H](O)[C@H](O)[C@H]6O)[C@@H]5O)[C@@H]4O)[C@H](O[C@H]4O[C@H](CO)[C@@H](O)[C@H](O[C@H]5O[C@H](CO)[C@@H](O)[C@H](O)[C@H]5O)[C@@H]4O)[C@@H]3O)[C@H](O[C@H]3O[C@H](CO)[C@@H](O)[C@H](O[C@H]4O[C@H](CO)[C@@H](O)[C@H](O)[C@H]4O)[C@@H]3O)[C@@H]2O)[C@H](O[C@H]2O[C@H](CO)[C@@H](O)[C@H](O[C@H]3O[C@H](CO)[C@@H](O)[C@H](O)[C@H]3O)[C@@H]2O)[C@@H]1O
Invalid SMILES [1*]O[C@H]1O[C@H](CO)[C@@H](O[C@H]2O[C@H](CO)[C@@H](O[C@H]3O[C@H](CO)[C@@H](O[C@H]4O[C@H](CO)[C@@H](O[C@H]5O[C@H](CO)[C@@H](O[C@H]6O[C@H](CO)[C@@H](O[C@H]7O[C@H](CO)[C@@H](O[C@H]8O[C@H](CO)[C@H](O)[C@H](O[C@H]9O[C@H](CO)[C@H](O)[C@H](O[C@H]%10O[C@H](CO)[C@H](O)[C@H](O[C@H]%11O[C@H](CO)[C@H](O)[C@H](O[C@@H]%12OC[C@@H](O[C@H]%13O[C@H](CO)[C@@H](O[C@H]%14O[C@H](CO)[C@@H](O[C@H]%15O[C@H](CO)[C@@H](O[C@H]%16O[C@H](CO)[C@@H](O[C@H]%17O[C@H](CO)[C@@H](O[C@@H]%18O[C@H](CO)[C@@H](O)[C@H](O[C@@H]%19O[C@H](CO)[C@@H](O)[C@H](O[C@@H] O[C@H](CO)[C@@H](O)[C@H](O[C@H]%21O[C@H](CO)[C@@H](O)[C@H](O)[C@H]%21O)[C@H] O)[C@H]%19O)[C@H]%18O)[C@H](O)[C@H]%17O)[C@H](O)[C@H]%16O)[C@H](O)[C@H]%15O)[C@H](O)[C@H]%14O)[C@H](O)[C@H]%13O)[C@H](O)[C@H]%12O)[C@H]%11O)[C@H]%10O)[C@H]9O)[C@H]8O)[C@H](O[C@H]8O[C@H](CO)[C@@H](O)[C@H](O)[C@@H]8O[C@H]8O[C@H](CO)[C@@H](O)[C@H](O)[C@H]8O)[C@@H]7O)[C@H](O[C@H]7O[C@H](CO)[C@@H](O)[C@H](O)[C@@H]7O[C@H]7O[C@H](CO)[C@@H](O)[C@H](O)[C@H]7O)[C@@H]6O)[C@H](O[C@H]6O[C@H](CO)[C@@H](O)[C@H](O[C@H]7O[C@H](CO)[C@@H](O)[C@H](O)[C@H]7O)[C@@H]6O)[C@@H]5O)[C@H](O[C@H]5O[C@H](CO)[C@@H](O)[C@H](O[C@H]6O[C@H](CO)[C@@H](O)[C@H](O)[C@H]6O)[C@@H]5O)[C@@H]4O)[C@H](O[C@H]4O[C@H](CO)[C@@H](O)[C@H](O[C@H]5O[C@H](CO)[C@@H](O)[C@H](O)[C@H]5O)[C@@H]4O)[C@@H]3O)[C@H](O[C@H]3O[C@H](CO)[C@@H](O)[C@H](O[C@H]4O[C@H](CO)[C@@H](O)[C@H](O)[C@H]4O)[C@@H]3O)[C@@H]2O)[C@H](O[C@H]2O[C@H](CO)[C@@H](O)[C@H](O[C@H]3O[C@H](CO)[C@@H](O)[C@H](O)[C@H]3O)[C@@H]2O)[C@@H]1O

Taxonomic group: fungi / Ascomycota (Phylum: Ascomycota)
Organ / tissue: mycelium

The structure was elucidated in this paper
Publication DOI: 10.1016/j.procbio.2022.10.022
Journal NLM ID: 9211419
Publisher: Barking, Essex: Elsevier Applied Science
Correspondence: Z. Zhu <zhyuanzhu

tust.edu.cn>
Institutions: State Key Laboratory of Food Nutrition and Safety, Tianjin University of Science and Technology, Tianjin, China, College of Food Science and Biotechnology, Tianjin University of Science and Technology, Tianjin, China

A homogeneous polysaccharide, ICMP-1, was isolated from the cultured mycelia of Isaria cicadae Miquel by hot water extraction. ICMP-1 (3.87 ×103 kDa) was a heteropolysaccharide mainly consisting of T-α-D-Glcp, →4)-α-D-Glcp-(1→, →4,6)-α-D-Manp-(1→, and →4)-β-D-Galp-(1→. In order to improve the antioxidant activity and the potential applications of ICMP-1, a typical ZnCl2 modification on ICMP-1 was carried out. Fourier-transform infrared spectroscopy (FT-IR), Energy dispersive spectrometer (EDS), scanning electron microscopy (SEM), atomic force microscope (AFM) and X-ray photoelectron spectroscopy (XPS) analysis confirmed that Zn was successfully introduced into the polymer chain of ICMP-1. The zinc modification derivative (ICMP-1-Zn) can alleviate H2O2-induced oxidative damage on PC12 cells. The stronger antioxidant activity of ICMP-1-Zn may be achieved by promoting the activity of zinc-containing enzymes, such as superoxide dismutase and lactate dehydrogenase.

polysaccharide, Structural characterization, Antioxidant activity, oxidative stress, Isaria cicadae Miquel, polysaccharide zinc complex

Structure type: structural motif or average structure ; 3870000
Location inside paper: Fig. 3f, table 2, ICMP-1
Trivial name: water-soluble polysaccharide ICMP-1
Contained glycoepitopes: IEDB_130701,IEDB_136044,IEDB_137472,IEDB_140629,IEDB_141794,IEDB_142488,IEDB_144983,IEDB_144998,IEDB_146664,IEDB_152206,IEDB_190606,IEDB_983930,IEDB_983931,SB_165,SB_166,SB_187,SB_192,SB_195,SB_44,SB_67,SB_7,SB_72,SB_88

Methods: 13C NMR, 1H NMR, NMR-2D, methylation, GC-MS, FTIR, composition analysis, HPLC, statistical analysis, cell viability assay, SEM, AFM, apoptosis assays, UV-vis, hot water extraction, EDS, XPS, LDH, SOD, GSH, synthesis of Zn derivative
Comments, role: NMR temperature was not specified; published NMR assignment is erroneous (impossible chemical shifts for C3 (81.73), С6 (62.54) of -4,6)aDManp; C4 (76.97) of -4)aDGlcp).

NCBI Taxonomy refs (TaxIDs): 218633
Show glycosyltransferases

NMR conditions: in D2O [as TSV]

13C NMR data:
Linkage	Residue	C1	C2	C3	C4	C5	C6
4,4	aDGlcp	99.70	71.70	73.25	?	71.45	60.50
4,6,4	aDGlcp	100.78	74.52	73.29	74.77	73.54	60.65
4,6	bDGalp	104.27	73.99	72.60	76.37	73.30	62.71
4	aDManp	106.15	76.54	?	72.75	73.17	?
	aDGlcp


1H NMR data:
Linkage	Residue	H1	H2	H3	H4	H5	H6
4,4	aDGlcp	5.31	3.56	3.74	3.59	3.87	3.75
4,6,4	aDGlcp	5.08	4.14	4.10	3.82	3.85	3.69
4,6	bDGalp	4.57	3.18	3.71	3.67	3.85	3.62
4	aDManp	5.05	4.12	3.79	3.57	3.61	3.54
	aDGlcp


1H/13C HSQC data:
Linkage	Residue	C1/H1	C2/H2	C3/H3	C4/H4	C5/H5	C6/H6
4,4	aDGlcp	99.70/5.31	71.70/3.56	73.25/3.74	?/3.59	71.45/3.87	60.50/3.75
4,6,4	aDGlcp	100.78/5.08	74.52/4.14	73.29/4.10	74.77/3.82	73.54/3.85	60.65/3.69
4,6	bDGalp	104.27/4.57	73.99/3.18	72.60/3.71	76.37/3.67	73.30/3.85	62.71/3.62
4	aDManp	106.15/5.05	76.54/4.12	?/3.79	72.75/3.57	73.17/3.61	?/3.54
	aDGlcp

¹H NMR data:

Linkage	Residue	H1	H2	H3	H4	H5	H6
4,4	aDGlcp	5.31	3.56	3.74	3.59	3.87	3.75
4,6,4	aDGlcp	5.08	4.14	4.10	3.82	3.85	3.69
4,6	bDGalp	4.57	3.18	3.71	3.67	3.85	3.62
4	aDManp	5.05	4.12	3.79	3.57	3.61	3.54
	aDGlcp

¹³C NMR data:

Linkage	Residue	C1	C2	C3	C4	C5	C6
4,4	aDGlcp	99.70	71.70	73.25	?	71.45	60.50
4,6,4	aDGlcp	100.78	74.52	73.29	74.77	73.54	60.65
4,6	bDGalp	104.27	73.99	72.60	76.37	73.30	62.71
4	aDManp	106.15	76.54	?	72.75	73.17	?
	aDGlcp

The spectrum also has 3 signals at unknown positions (not plotted).

There is only one chemically distinct structure:

[*]O[C@@H]1[C@@H](CO)O[C@H](O[C@@H]2[C@@H](CO)O[C@H](O[C@@H]3[C@@H](CO)O[C@H](O[C@@H]4[C@@H](CO)O[C@H](O[C@@H]5[C@@H](CO)O[C@H](O[C@@H]6[C@@H](CO[C@@H]7O[C@H](CO)[C@H](O[C@H]8O[C@H](CO)[C@@H](O)[C@H](O)[C@H]8O)[C@H](O)[C@H]7O)O[C@H](O[C@@H]7[C@@H](CO)O[C@H]([*])[C@H](O)[C@H]7O)[C@@H](O)[C@H]6O)[C@H](O)[C@H]5O)[C@H](O)[C@H]4O)[C@H](O)[C@H]3O)[C@H](O)[C@H]2O)[C@H](O)[C@H]1O

SVG MW SMILES 3D mol Ionize

Warning = sub-repeat ending at aDGlcp: large unit count (6) was reduced to 5