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Taxonomic group: fungi / Ascomycota (Phylum: Ascomycota)
Organ / tissue: mycelia

The structure was elucidated in this paper
NCBI PubMed ID: 36983458
Publication DOI: 10.3390/jof9030291
Journal NLM ID: 101671827
Publisher: Basel, Switzerland: MDPI AG
Correspondence: M.I.D.D.S. Xisto <marylanax

gmail.com>; E. Barreto-Bergter <eliana.bergter

micro.ufrj.br>
Institutions: Laboratório de Química Biológica de Microrganismos, Departamento de Microbiologia Geral, Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro (UFRJ), Rio de Janeiro 21941-902, Brazil, Department of Pediatric, School of Medicine and Public Health, University of Wisconsin-Madison, Madison, WI 53706-1521, USA, Laboratório de Tecido Conjuntivo, Hospital Universitário Clementino Fraga Filho and Instituto de Bioquímica Médica Leopoldo de Meis, Universidade Federal do Rio de Janeiro (UFRJ), Rio de Janeiro 21941-913, Brazil, Departamento de Microbiologia e Parasitologia, Instituto Biomédico, Universidade Federal do Estado do Rio de Janeiro, Rio de Janeiro 20211-010, Brazil

Scedosporium and Lomentospora are a group of filamentous fungi with some clinically relevant species causing either localized, invasive, or disseminated infections. Understanding how the host immune response is activated and how fungi interact with the host is crucial for a better management of the infection. In this context, an α-glucan has already been described in S. boydii, which plays a role in the inflammatory response. In the present study, an α-glucan has been characterized in L. prolificans and was shown to be exposed on the fungal surface. The α-glucan is recognized by peritoneal macrophages and induces oxidative burst in activated phagocytes. Its recognition by macrophages is mediated by receptors that include Dectin-1 and Mincle, but not TLR2 and TLR4. These results contribute to the understanding of how Scedosporium's and Lomentospora's physiopathologies are developed in patients suffering with scedosporiosis and lomentosporiosis.

toll-like receptors, α-glucan, C-type lectin receptors, Lomentospora prolificans, Scedosporium boydii

Structure type: homopolymer
Location inside paper: table 1, Fig. 1C, L. prolificans
Trivial name: methyl glucose lipopolysaccharide, glucan, maltosaccharide, α-1,4-D-glucan, amylose, α-glucan, glycogen backbone, α-(1,4)-glucan, starch, α-(1-4)-glucan, starch, glycogen
Compound class: EPS, O-polysaccharide, CPS, cell wall polysaccharide, glucan, polysaccharide, methyl glucose lipopolysaccharide
Contained glycoepitopes: IEDB_140629,IEDB_142488,IEDB_144998,IEDB_146664,IEDB_420417,IEDB_420418,IEDB_420421,IEDB_857742,IEDB_983931,SB_192

Methods: 13C NMR, 1H NMR, NMR-2D, ELISA, cytokine analysis, HPTLC, statistical analysis, flow cytometry analysis, cytotoxicity assay, TLR4 activation assay, phagocytosis assay, animal experiments, alkaline extraction, a-amyloglucosidase treatment

Related record ID(s): 41515
NCBI Taxonomy refs (TaxIDs): 41688
Reference(s) to other database(s): GTC:G05740LL, GlycomeDB:12100, CCSD:4943, CBank-STR:819
Show glycosyltransferases

NMR conditions: in D2O at 313 K [as TSV]

13C NMR data:
Linkage	Residue	C1	C2	C3	C4	C5	C6
	aDGlcp	100.1	71.8	73.5	77.6	71.7	60.9


1H NMR data:
Linkage	Residue	H1	H2	H3	H4	H5	H6
	aDGlcp	5.38	3.64	3.97	3.64	3.85	3.77-3.87


1H/13C HSQC data:
Linkage	Residue	C1/H1	C2/H2	C3/H3	C4/H4	C5/H5	C6/H6
	aDGlcp	100.1/5.38	71.8/3.64	73.5/3.97	77.6/3.64	71.7/3.85	60.9/3.77-3.87

¹H NMR data:

Linkage	Residue	H1	H2	H3	H4	H5	H6
	aDGlcp	5.38	3.64	3.97	3.64	3.85	3.77 3.87

¹³C NMR data:

Linkage	Residue	C1	C2	C3	C4	C5	C6
	aDGlcp	100.1	71.8	73.5	77.6	71.7	60.9

There is only one chemically distinct structure:

[*]O[C@@H]1[C@@H](CO)O[C@H]([*])[C@H](O)[C@H]1O

SVG MW SMILES 3D mol Ionize

gmail.com>; E. Barreto-Bergter <eliana.bergter

toll-like receptors, α-glucan, C-type lectin receptors, Lomentospora prolificans, Scedosporium boydii

Structure type: polymer chemical repeating unit
Location inside paper: table 1, Fig. 1C, S. boydii
Trivial name: α-glucan

Methods: 13C NMR, 1H NMR, NMR-2D, ELISA, cytokine analysis, HPTLC, statistical analysis, flow cytometry analysis, cytotoxicity assay, TLR4 activation assay, phagocytosis assay, animal experiments, alkaline extraction, a-amyloglucosidase treatment
Comments, role: in the published NMR assignment, C6 A' and C6 B signals were erroneusly swapped; erroneous C4 A' signal at 77.0 was reassigned to C4 B by CSDB staff; suggestedly erroneous C4 B signal (73.4) was removed by CSDB staff.

Related record ID(s): 41101
NCBI Taxonomy refs (TaxIDs): 5597
Show glycosyltransferases

NMR conditions: in D2O at 313 K [as TSV]

13C NMR data:
Linkage	Residue	C1	C2	C3	C4	C5	C6
4,4,6,4	aDGlcp	100.1	71.8	73.5	77.6	71.7	60.9
4,4,4	aDGlcp	100.1	71.8	73.5	77.6	71.7	60.9
4,4,6,4,4	aDGlcp	100.1	71.8	73.6	?	70.6	60.9
4,4,6	aDGlcp
4,4	aDGlcp	98.9	73.4	70.6	77.0	?	67.6
4	aDGlcp
	aDGlcp	100.1	71.8	73.5	77.6	71.7	60.9


1H NMR data:
Linkage	Residue	H1	H2	H3	H4	H5	H6
4,4,6,4	aDGlcp	5.38	3.64	3.97	3.64	3.85	3.77-3.87
4,4,4	aDGlcp	5.38	3.64	3.97	3.64	3.85	3.77-3.87
4,4,6,4,4	aDGlcp	5.34	3.60	4.02	3.62	3.88	3.87-3.96
4,4,6	aDGlcp
4,4	aDGlcp	4.97	3.59	4.03	3.66	?	3.77-3.87
4	aDGlcp
	aDGlcp	5.38	3.64	3.97	3.64	3.85	3.77-3.87


1H/13C HSQC data:
Linkage	Residue	C1/H1	C2/H2	C3/H3	C4/H4	C5/H5	C6/H6
4,4,6,4	aDGlcp	100.1/5.38	71.8/3.64	73.5/3.97	77.6/3.64	71.7/3.85	60.9/3.77-3.87
4,4,4	aDGlcp	100.1/5.38	71.8/3.64	73.5/3.97	77.6/3.64	71.7/3.85	60.9/3.77-3.87
4,4,6,4,4	aDGlcp	100.1/5.34	71.8/3.60	73.6/4.02	?/3.62	70.6/3.88	60.9/3.87-3.96
4,4,6	aDGlcp
4,4	aDGlcp	98.9/4.97	73.4/3.59	70.6/4.03	77.0/3.66	?/?	67.6/3.77-3.87
4	aDGlcp
	aDGlcp	100.1/5.38	71.8/3.64	73.5/3.97	77.6/3.64	71.7/3.85	60.9/3.77-3.87

¹H NMR data:

Linkage	Residue	H1	H2	H3	H4	H5	H6
4,4,6,4	aDGlcp	5.38	3.64	3.97	3.64	3.85	3.77 3.87
4,4,4	aDGlcp	5.38	3.64	3.97	3.64	3.85	3.77 3.87
4,4,6,4,4	aDGlcp	5.34	3.60	4.02	3.62	3.88	3.87 3.96
4,4,6	aDGlcp
4,4	aDGlcp	4.97	3.59	4.03	3.66	?	3.77 3.87
4	aDGlcp
	aDGlcp	5.38	3.64	3.97	3.64	3.85	3.77 3.87

¹³C NMR data:

Linkage	Residue	C1	C2	C3	C4	C5	C6
4,4,6,4	aDGlcp	100.1	71.8	73.5	77.6	71.7	60.9
4,4,4	aDGlcp	100.1	71.8	73.5	77.6	71.7	60.9
4,4,6,4,4	aDGlcp	100.1	71.8	73.6	?	70.6	60.9
4,4,6	aDGlcp
4,4	aDGlcp	98.9	73.4	70.6	77.0	?	67.6
4	aDGlcp
	aDGlcp	100.1	71.8	73.5	77.6	71.7	60.9

The spectrum also has 2 signals at unknown positions (not plotted).

There is only one chemically distinct structure:

[*]O[C@@H]1[C@@H](CO)O[C@H](O[C@@H]2[C@@H](CO[C@H]3O[C@H](CO)[C@@H](O[C@H]4O[C@H](CO)[C@@H](O[C@H]5O[C@H](CO)[C@@H](O[C@H]6O[C@H](CO)[C@@H](O[C@H]7O[C@H](CO)[C@@H](O)[C@H](O)[C@H]7O)[C@H](O)[C@H]6O)[C@H](O)[C@H]5O)[C@H](O)[C@H]4O)[C@H](O)[C@H]3O)O[C@H](O[C@@H]3[C@@H](CO)O[C@H](O[C@@H]4[C@@H](CO)O[C@H]([*])[C@H](O)[C@H]4O)[C@H](O)[C@H]3O)[C@H](O)[C@H]2O)[C@H](O)[C@H]1O

SVG MW SMILES 3D mol Ionize

Warning = sub-repeat ending at aDGlcp: unknown unit count was assumed to be 3