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Taxonomic group: fungi / Basidiomycota (Phylum: Basidiomycota)
Organ / tissue: fruiting bodies

NCBI PubMed ID: 37595710
Publication DOI: 10.1016/j.ijbiomac.2023.126357
Journal NLM ID: 7909578
Publisher: Butterworth-Heinemann
Correspondence: K. Wang <wkpzcq

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Institutions: Hubei Key Laboratory of Nature Medicinal Chemistry and Resource Evaluation, Tongji Medical College of Pharmacy, Huazhong University of Science and Technology, 430030 Wuhan, China, Department of Pharmacy, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, 430030 Wuhan, China, Hubei province clinical research center for precision medicine for critical illness, 430030 Wuhan, China

Grifola frondosa polysaccharide (GFP) is mainly composed of α-1,4 glycosidic bonds and possesses multiple pharmacological activities. However, the absence of pharmacokinetic studies has limited its further development and utilization. Herein, GFP was labeled with 5-DTAF (FGFP) and cyanine 5.5 amine (GFP-Cy5.5) to investigate its gastrointestinal metabolism characteristics and mechanism. Significant distributions of the polysaccharide in the liver and kidneys were observed by near infrared imaging. To investigate the specific distribution form of the polysaccharide, in vitro digestion models were constructed and revealed that FGFP was degraded in saliva and rat small intestine extract. The metabolites were detected in the stomach and small intestine, followed by further degradation in the distal intestine in the in vivo experiment. Subsequent investigations showed that α-amylase was involved in the gastrointestinal degradation of GFP, and its metabolite finally entered the kidneys, where it was excreted directly with urine.

distribution, fluorescent labeling, Grifola frondosa polysaccharide, excretion, gastrointestinal metabolism

Structure type: structural motif or average structure
Location inside paper: Fig. 1, Fig. 2A, GFP
Trivial name: polysaccharide (GFP)
Compound class: polysaccharide, glucogalactomannan
Contained glycoepitopes: IEDB_130701,IEDB_136044,IEDB_137472,IEDB_140629,IEDB_141794,IEDB_141806,IEDB_141836,IEDB_142487,IEDB_142488,IEDB_144983,IEDB_144998,IEDB_146664,IEDB_152206,IEDB_190606,IEDB_420417,IEDB_420421,IEDB_857742,IEDB_983930,IEDB_983931,SB_165,SB_166,SB_187,SB_192,SB_195,SB_44,SB_6,SB_67,SB_7,SB_72,SB_88

Methods: sugar analysis, statistical analysis, fluorescence labeling, animal experiments, boiling water extraction, NIR imaging, gastrointestinal metabolism, In vitro digestion of FGFP

NCBI Taxonomy refs (TaxIDs): 5627
Show glycosyltransferases

There is only one chemically distinct structure:

[*]O[C@@H]1[C@@H](CO)O[C@H](O[C@@H]2[C@@H](CO)O[C@H](O[C@@H]3[C@@H](CO)O[C@H](O[C@@H]4[C@@H](CO)O[C@H](O[C@@H]5[C@@H](CO)O[C@H](O[C@@H]6[C@H](O)[C@@H](O[C@@H]7[C@@H](CO)O[C@H](O[C@H]8[C@@H](CO[C@@H]9O[C@H](CO)[C@@H](O)[C@H](O)[C@H]9O)O[C@@H]([*])[C@H](O)[C@H]8O)[C@H](O)[C@H]7O)O[C@H](CO[C@H]7O[C@H](CO[C@@H]8O[C@H](CO)[C@@H](O)[C@H](O)[C@H]8O)[C@@H](O)[C@H](O)[C@H]7O)[C@H]6O)[C@H](O)[C@H]5O)[C@H](O)[C@H]4O)[C@H](O)[C@H]3O)[C@H](O)[C@H]2O)[C@H](O)[C@H]1O

SVG MW SMILES 3D mol Ionize