Taxonomic group: fungi / Ascomycota
(Phylum: Ascomycota)
The structure was elucidated in this paperNCBI PubMed ID: 16359651Publication DOI: 10.1016/j.carres.2005.10.013Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: Seviour RJ <r.seviour
latrobe.edu.au>
Institutions: School of Agriculture and Wine, The University of Adelaide, Glen Osmond, Australia, School of Biochemistry, La Trobe University, Bundoora, Australia, Department of Chemistry, La Trobe University, Bundoora, Australia, Biotechnology Research Centre, La Trobe University, Bendigo, Australia
In a previous article [Carbohydr. Res.2001, 331, 163-171] two different structures for the possible modular repeating unit of the extracellular beta-glucan, epiglucan produced by the fungus Epicoccum nigrum strain F19 were proposed. Clarifying which was the more likely one was considered essential before attempts were made to understand how epiglucan was assembled by this fungus. Data from Smith degradation analyses of epiglucan were consistent with the repeating unit of structure I, where single glucosyl residues are attached by (1-->6)-beta-linkages to two out of every three glucosyl residues in the (1-->3)-beta-linked glucan backbone. Repeated Smith degradations of 14C-glucose labelled epiglucan showed that chain elongation occurred from its non-reducing end. Side chain insertion into the growing glucan was followed by analysis of real time incorporation of 13C-glucose into epiglucan by 13C NMR, and 14C-glucose by enzymic digestion of the synthesised 14C-epiglucan. All data obtained were consistent with the view that single (1-->6)-beta-linked glucosyl side residues are inserted simultaneously as the glucan backbone elongates.
biosynthesis, NMR spectroscopy, extracellular polysaccharide, β-Glucan assembly, Epiglucan elongation, Side chain insertion, Radioisotope labelling
Structure type: polymer chemical repeating unit
Location inside paper: abstract, p. 366, Fig. 2, epiglucan I
Trivial name: epiglucan
Compound class: EPS
Contained glycoepitopes: IEDB_1397514,IEDB_141806,IEDB_142488,IEDB_146664,IEDB_153543,IEDB_158555,IEDB_161166,IEDB_241101,IEDB_558869,IEDB_857743,IEDB_983931,SB_192
Methods: 13C NMR, TLC, acid hydrolysis, HPAEC, Smith degradation, radiolabeling, enzymatic digestion, extraction, 14C-scintillation counting
NCBI Taxonomy refs (TaxIDs): 105696Reference(s) to other database(s): GTC:G22852XL
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
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