Taxonomic group: fungi / Basidiomycota
(Phylum: Basidiomycota)
Organ / tissue: mycelium
The structure was elucidated in this paperNCBI PubMed ID: 16678806Publication DOI: 10.1016/j.carres.2006.03.039Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: Zhang J <zhangjs888
etang.com>, Pan Y <yjpan
shfu.edu.cn>
Institutions: Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Shanghai, China, College of Food Science, Shanghai Fisheries University, Shanghai, China, College of Life Sciences, Nanjing Agricultural University, Nanjing, China
A water-soluble fucogalactan (CMP3), with a molecular mass of 1.03 x 10(4) Da as determined by high-performance size-exclusion chromatography (HPSEC), was obtained from the crude intracellular polysaccharide of Coprinus comatus mycelium. Its chemical structure was characterized by sugar and methylation analysis along with 1H and 13C NMR spectroscopy, including NOESY and HMBC experiments for linkage and sequence analysis. The polysaccharide is composed of a pentasaccharide repeating unit with the following structure: [structure:see text].
polysaccharide, structural analysis, NMR spectroscopy, mycelium, fucogalactan, Coprinus comatus
Structure type: polymer chemical repeating unit ; 10300
Location inside paper: abstract, p. 1132, table 1
Trivial name: fucogalactan
Compound class: EPS, fucogalactan
Contained glycoepitopes: IEDB_134624,IEDB_136045,IEDB_136906,IEDB_137472,IEDB_141794,IEDB_142489,IEDB_144562,IEDB_151528,IEDB_152214,IEDB_153553,IEDB_174333,IEDB_190606,SB_154,SB_163,SB_7,SB_86
Methods: 13C NMR, 1H NMR, NMR-2D, methylation, GC-MS, sugar analysis, acid hydrolysis, GLC, HPLC, HPSEC, extraction, dialysis, HPAEC–PAD
Comments, role: 1H NMR temperature: 298 and 333
Related record ID(s): 45049
NCBI Taxonomy refs (TaxIDs): 56187Reference(s) to other database(s): GTC:G71379MN
Show glycosyltransferases
NMR conditions: in D2O at 298(C), 333(H) K
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6
6,6,6 aDGalp 100.70 71.02 72.29 72.59 71.94 69.82
6,6,2 aLFucp 104.07 72.2 71.02 74.48 69.82 18.33
6,6 aDGalp 100.7 80.65 71.14 72.29 71.40 69.32
6 aDGalp 100.7 71.02 72.29 72.20 71.50 69.58
aDGalp 100.70 71.02 72.29 72.59 71.94 69.82
1H NMR data:
Linkage Residue H1 H2 H3 H4 H5 H6
6,6,6 aDGalp 5.02 3.89 3.92 4.05 4.17 3.68-4.01
6,6,2 aLFucp 5.12 3.85 3.93 3.89 4.20 1.28
6,6 aDGalp 5.08 3.87 4.10 4.12 4.23 3.74-3.95
6 aDGalp 5.03 3.89 3.92 4.07 4.23 3.74-3.95
aDGalp 5.01 3.89 3.92 4.04 4.17 3.68-4.01
1H/13C HSQC data:
Linkage Residue C1/H1 C2/H2 C3/H3 C4/H4 C5/H5 C6/H6
6,6,6 aDGalp 100.70/5.02 71.02/3.89 72.29/3.92 72.59/4.05 71.94/4.17 69.82/3.68-4.01
6,6,2 aLFucp 104.07/5.12 72.2/3.85 71.02/3.93 74.48/3.89 69.82/4.20 18.33/1.28
6,6 aDGalp 100.7/5.08 80.65/3.87 71.14/4.10 72.29/4.12 71.40/4.23 69.32/3.74-3.95
6 aDGalp 100.7/5.03 71.02/3.89 72.29/3.92 72.20/4.07 71.50/4.23 69.58/3.74-3.95
aDGalp 100.70/5.01 71.02/3.89 72.29/3.92 72.59/4.04 71.94/4.17 69.82/3.68-4.01
1H NMR data:
| Linkage | Residue | H1 | H2 | H3 | H4 | H5 | H6 |
|---|
| 6,6,6 | aDGalp | 5.02 | 3.89 | 3.92 | 4.05 | 4.17 | 3.68
4.01 |
| 6,6,2 | aLFucp | 5.12 | 3.85 | 3.93 | 3.89 | 4.20 | 1.28 |
| 6,6 | aDGalp | 5.08 | 3.87 | 4.10 | 4.12 | 4.23 | 3.74
3.95 |
| 6 | aDGalp | 5.03 | 3.89 | 3.92 | 4.07 | 4.23 | 3.74
3.95 |
| | aDGalp | 5.01 | 3.89 | 3.92 | 4.04 | 4.17 | 3.68
4.01 |
|
13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 |
|---|
| 6,6,6 | aDGalp | 100.70 | 71.02 | 72.29 | 72.59 | 71.94 | 69.82 |
| 6,6,2 | aLFucp | 104.07 | 72.2 | 71.02 | 74.48 | 69.82 | 18.33 |
| 6,6 | aDGalp | 100.7 | 80.65 | 71.14 | 72.29 | 71.40 | 69.32 |
| 6 | aDGalp | 100.7 | 71.02 | 72.29 | 72.20 | 71.50 | 69.58 |
| | aDGalp | 100.70 | 71.02 | 72.29 | 72.59 | 71.94 | 69.82 |
|
There is only one chemically distinct structure:
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