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1. (CSDB ID: 42364) | report error |
| -4)-b-D-GlcpNAc-(1- | Show graphically |
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Saccharomyces cerevisiae
(NCBI TaxID 4932,
species name lookup)
ua.ptBrewers spent yeast insoluble residue (BSYIR) is the insoluble material that remains after the exhaustive extraction with strong alkali solutions of brewers spent yeast (BSY). BSYIR contains 57% polysaccharides, composed mainly of glucose residues (98%) and minor amount of mannose residues (1%). The glucans were mainly (1→4)-linked (56.6%); with lower amounts of (1→3)-, (1→4,6)-, terminal-, (1→6)-, and (1→3,6)-linked glucose residues, together with terminal- and (1→6)-linked mannose residues. Scanning electron microscopy (SEM) showed that in BSYIR the spherical shape of BSY was preserved. However, when the BSYIR was treated with chlorite solutions, either under alkali or acidic conditions, followed by an extraction with aqueous KOH (0.1 M) solution, it promoted the destruction of the cell wall three-dimensional structure. The treatments with chlorite solutions allowed the solubilisation of yeast non carbohydrate material in BSYIR and the recovered residues were enriched in carbohydrates. The residue left after treatment with chlorite under alkaline solutions was composed of 92% of carbohydrates and the residue left with chlorite/acetic acid treatment contained 89% carbohydrate. Both residues were composed of only glucose. Comparing the BSYIR with the residue obtained after treatment with chlorite under alkaline solutions, it was observed an enrichment in linear glucans, (1→4)-Glc (61%) and (1→3)-Glc (from 17% to 20%), and the decrease in branched glucans, namely (1→4,6)-Glc, from 10% to 4%. The results obtained show that the three-dimensional spherical structure of the yeast cell wall is preserved by a network of glucans and mannoproteins. The oxidation of this network promotes its disruption by formation of an unbranched compact structure of glucans.
glycoproteins, Glucans, Saccharomyces pastorianus, scanning electronmicroscopy(SEM)
Structure type: structural motif or average structure|
2. (CSDB ID: 42365) | report error |
| {{{-a-D-Glc-(1-4)-}}}?%a-D-Glcp-(1-6)-+ | -4)-a-D-Glcp-(1- | Show graphically |
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Saccharomyces cerevisiae
(NCBI TaxID 4932,
species name lookup)
Saccharomyces pastorianus
(NCBI TaxID 27292,
species name lookup)
ua.ptBrewers spent yeast insoluble residue (BSYIR) is the insoluble material that remains after the exhaustive extraction with strong alkali solutions of brewers spent yeast (BSY). BSYIR contains 57% polysaccharides, composed mainly of glucose residues (98%) and minor amount of mannose residues (1%). The glucans were mainly (1→4)-linked (56.6%); with lower amounts of (1→3)-, (1→4,6)-, terminal-, (1→6)-, and (1→3,6)-linked glucose residues, together with terminal- and (1→6)-linked mannose residues. Scanning electron microscopy (SEM) showed that in BSYIR the spherical shape of BSY was preserved. However, when the BSYIR was treated with chlorite solutions, either under alkali or acidic conditions, followed by an extraction with aqueous KOH (0.1 M) solution, it promoted the destruction of the cell wall three-dimensional structure. The treatments with chlorite solutions allowed the solubilisation of yeast non carbohydrate material in BSYIR and the recovered residues were enriched in carbohydrates. The residue left after treatment with chlorite under alkaline solutions was composed of 92% of carbohydrates and the residue left with chlorite/acetic acid treatment contained 89% carbohydrate. Both residues were composed of only glucose. Comparing the BSYIR with the residue obtained after treatment with chlorite under alkaline solutions, it was observed an enrichment in linear glucans, (1→4)-Glc (61%) and (1→3)-Glc (from 17% to 20%), and the decrease in branched glucans, namely (1→4,6)-Glc, from 10% to 4%. The results obtained show that the three-dimensional spherical structure of the yeast cell wall is preserved by a network of glucans and mannoproteins. The oxidation of this network promotes its disruption by formation of an unbranched compact structure of glucans.
glycoproteins, Glucans, Saccharomyces pastorianus, scanning electronmicroscopy(SEM)
Structure type: structural motif or average structure|
3. (CSDB ID: 42366) | report error |
| -4)-b-D-Glcp-(1- | Show graphically |
|
Show legend Show as text |
Saccharomyces pastorianus
(NCBI TaxID 27292,
species name lookup)
ua.ptBrewers spent yeast insoluble residue (BSYIR) is the insoluble material that remains after the exhaustive extraction with strong alkali solutions of brewers spent yeast (BSY). BSYIR contains 57% polysaccharides, composed mainly of glucose residues (98%) and minor amount of mannose residues (1%). The glucans were mainly (1→4)-linked (56.6%); with lower amounts of (1→3)-, (1→4,6)-, terminal-, (1→6)-, and (1→3,6)-linked glucose residues, together with terminal- and (1→6)-linked mannose residues. Scanning electron microscopy (SEM) showed that in BSYIR the spherical shape of BSY was preserved. However, when the BSYIR was treated with chlorite solutions, either under alkali or acidic conditions, followed by an extraction with aqueous KOH (0.1 M) solution, it promoted the destruction of the cell wall three-dimensional structure. The treatments with chlorite solutions allowed the solubilisation of yeast non carbohydrate material in BSYIR and the recovered residues were enriched in carbohydrates. The residue left after treatment with chlorite under alkaline solutions was composed of 92% of carbohydrates and the residue left with chlorite/acetic acid treatment contained 89% carbohydrate. Both residues were composed of only glucose. Comparing the BSYIR with the residue obtained after treatment with chlorite under alkaline solutions, it was observed an enrichment in linear glucans, (1→4)-Glc (61%) and (1→3)-Glc (from 17% to 20%), and the decrease in branched glucans, namely (1→4,6)-Glc, from 10% to 4%. The results obtained show that the three-dimensional spherical structure of the yeast cell wall is preserved by a network of glucans and mannoproteins. The oxidation of this network promotes its disruption by formation of an unbranched compact structure of glucans.
glycoproteins, Glucans, Saccharomyces pastorianus, scanning electronmicroscopy(SEM)
Structure type: homopolymer| New query | Export IDs | Home | Help |
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