Taxonomic group: fungi / Basidiomycota
(Phylum: Basidiomycota)
The structure was elucidated in this paperNCBI PubMed ID: 19054236Publication DOI: 10.1111/j.1365-2672.2008.03995.xJournal NLM ID: 9706280Publisher: Oxford: Blackwell Publishing for the Society for Applied Bacteriology
Correspondence: Fariña JI <jifarina
yahoo.com>; Fariña JI <jifarina
proimi.org.ar>
Institutions: PROIMI-CONICET (Planta Piloto de Procesos Industriales Microbiológicos), Tucumán, Argentina, Instituto de Química Física, Facultad de Bioquímica, Química y Farmacia, Tucumán, Argentina, Cátedra de Microbiología Superior, Facultad de Bioquıímica, Química y Farmacia, UNT, Tucumán, Argentina
Sclerotium rolfsii ATCC 201126 exopolysaccharides (EPSs) recovered at 48 h (EPS I) and 72 h (EPS II) of fermentation, with differences in rheological parameters, hydrogel topography, salt tolerance, antisyneresis, emulsifying and suspending properties, were subjected to a polyphasic characterization in order to detect structural divergences. METHODS AND RESULTS: Fermenter-scale production led to productivity (P(r)) and yield (Y(P/C)) values higher at 48 h (P(r) = 0.542 g l(-1) h(-1); Y(P/C) = 0.74) than at 72 h (P(r) = 0.336 g l(-1) h(-1); Y(P/C) = 0.50). Both EPSs were neutral glucose-homopolysaccharides with a beta-(1,3)-glycosidic backbone and single beta-(1,6)-glucopyranosyl sidechains regularly attached every three residues in the main chain, as revealed by chemical analyses. The infra-red diagnostic peak at 890 cm(-1) confirmed beta-glycosidic linkages, while gentiobiose released by beta-(1,3)-glucanases confirmed single beta-1,6-glycosidic branching for both EPSs. The true modular repeating unit of S. rolfsii ATCC 201126 scleroglucan could be resolved. Structural stability was corroborated and no structural differences could be detected as to account for the variations in EPSs behaviour. Recovery of S. rolfsii ATCC 201126 scleroglucan at 48 h might be considered based on better fermentation kinetic parameters and no detrimental effects on EPS structural features.
structure, fermentation, scleroglucan, Sclerotium rolfsii, enzymatic digestion, FT-IR
Structure type: polymer chemical repeating unit ; 1600000-1700000; n=2400-2500
Location inside paper: abstract, EPS I, EPS II
Trivial name: scleroglucan
Compound class: EPS, glucan
Contained glycoepitopes: IEDB_1397514,IEDB_141806,IEDB_142488,IEDB_146664,IEDB_153543,IEDB_158555,IEDB_161166,IEDB_241101,IEDB_558869,IEDB_857743,IEDB_983931,SB_192
Methods: TLC, acid hydrolysis, Smith degradation, FTIR, HPLC, periodate oxidation, PC, enzymatic treatments, precipitation, phenol-sulfuric acid assay, centrifugation, the Somogyi-Nelson method, ANOVA, Folin-Lowry method
Enzymes that release or process the structure: cellulase, Novozym 234, zymolyase
Related record ID(s): 50040, 50086, 50627, 50647, 50649
NCBI Taxonomy refs (TaxIDs): 39291Reference(s) to other database(s): GTC:G66305IS
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There is only one chemically distinct structure:
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(later renamed to: Athelia rolfsii ATCC 201126)