Taxonomic group: fungi / Basidiomycota
(Phylum: Basidiomycota)
The structure was elucidated in this paperNCBI PubMed ID: 18800181Publication DOI: 10.1007/s00253-008-1700-2Journal NLM ID: 8406612Publisher: Springer
Correspondence: Rau U <U.Rau
tu-bs.de>
Institutions: Institute of Biochemistry and Biotechnology, Technical University Braunschweig, Braunschweig, Germany, Institute of Agricultural Technology and Biosystems Engineering, Johann Heinrich von Thünen-Institut, Braunschweig, Germany, Department of Structure Biology, Helmholtz Centre for Infection Research, Braunschweig, Germany
Trametes versicolor ATCC 200801 secretes 4.1 g L(-1) of exopolysaccharide (EPS) when synthetic minimal medium and low-shear bioreactor cultivation technique are used. Structural and compositional analyses by thin layer chromatography, gas chromatography-mass spectrometry, electrospray ionization tandem mass spectrometry, and nuclear magnetic resonance spectroscopy yielded predominantly glucose and small amounts of galactose, mannose, arabinose, and xylose. The main EPS is composed of beta-1,3/beta-1,6-linked D-glucose molecules which is identical with Schizophyllan but does not possess a triple helical arrangement as secondary structure. Two molar mass fractions were detected by size exclusion chromatography yielding weight-average molecular weights of 4,100 and 2.6 kDa. Protein content varies between 2-3.6% (w/w). The exopolysaccharide is different in the nature of the glycosidic linkage, composition of monosaccharides, protein content, and weight-average molecular weight compared to the well-known polysaccharopeptide (PSP) and polysaccharopeptide Krestin (PSK).
polysaccharide, viscosity, molecular weight, structure analysis, Trametes versicolor, bioreactor cultivation
Structure type: polymer chemical repeating unit ; 2600-4100000
Location inside paper: fig. 5, EPS, in text
Compound class: EPS, glucan
Contained glycoepitopes: IEDB_1397514,IEDB_141806,IEDB_142488,IEDB_146664,IEDB_153543,IEDB_158555,IEDB_161166,IEDB_241101,IEDB_558869,IEDB_857743,IEDB_983931,SB_192
Methods: 13C NMR, 1H NMR, NMR-2D, IR, partial acid hydrolysis, GC-MS, TLC, enzymatic hydrolysis, ESI-MS, acid hydrolysis, FTIR, methanolysis, HPSEC, viscosity measurement, extraction, NaBH4 reduction, SEC, reduction with NaBH4, protease treatment, HMBC, COSY, trimethylsilylation, TFA hedrolysis, sulphuric acid hydrolysis
Synthetic data: biosynthesis
Comments, role: EPS was produced by T. versicolor ATCC 200801 cultivated in glucose medium
NCBI Taxonomy refs (TaxIDs): 5325Reference(s) to other database(s): GTC:G66305IS
Show glycosyltransferases
NMR conditions: in (13C) / (1H) at 353(C) K
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6
3,3 bDGlcp 102.6-102.8 72.3-73.3 85.7-86.2 68.2-68.4 74.6-76.3 60.6-60.9
3,6 bDGlcp 102.6-102.8 72.3-73.3 74.6-76.3 70.1 74.6-76.3 60.6-60.9
3 bDGlcp 102.6-102.8 72.3-73.3 85.7-86.2 68.2-68.4 74.6-76.3 68.1
bDGlcp 102.6-102.8 72.3-73.3 85.7-86.2 68.2-68.4 74.6-76.3 60.6-60.9
1H NMR data:
missing...
13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 |
|---|
| 3,3 | bDGlcp | 102.6
102.8 | 72.3
73.3 | 85.7
86.2 | 68.2
68.4 | 74.6
76.3 | 60.6
60.9 |
| 3,6 | bDGlcp | 102.6
102.8 | 72.3
73.3 | 74.6
76.3 | 70.1 | 74.6
76.3 | 60.6
60.9 |
| 3 | bDGlcp | 102.6
102.8 | 72.3
73.3 | 85.7
86.2 | 68.2
68.4 | 74.6
76.3 | 68.1 |
| | bDGlcp | 102.6
102.8 | 72.3
73.3 | 85.7
86.2 | 68.2
68.4 | 74.6
76.3 | 60.6
60.9 |
|
There is only one chemically distinct structure:
Taxonomic group: fungi / Basidiomycota
(Phylum: Basidiomycota)
The structure was elucidated in this paperNCBI PubMed ID: 18800181Publication DOI: 10.1007/s00253-008-1700-2Journal NLM ID: 8406612Publisher: Springer
Correspondence: Rau U <U.Rau
tu-bs.de>
Institutions: Institute of Biochemistry and Biotechnology, Technical University Braunschweig, Braunschweig, Germany, Institute of Agricultural Technology and Biosystems Engineering, Johann Heinrich von Thünen-Institut, Braunschweig, Germany, Department of Structure Biology, Helmholtz Centre for Infection Research, Braunschweig, Germany
Trametes versicolor ATCC 200801 secretes 4.1 g L(-1) of exopolysaccharide (EPS) when synthetic minimal medium and low-shear bioreactor cultivation technique are used. Structural and compositional analyses by thin layer chromatography, gas chromatography-mass spectrometry, electrospray ionization tandem mass spectrometry, and nuclear magnetic resonance spectroscopy yielded predominantly glucose and small amounts of galactose, mannose, arabinose, and xylose. The main EPS is composed of beta-1,3/beta-1,6-linked D-glucose molecules which is identical with Schizophyllan but does not possess a triple helical arrangement as secondary structure. Two molar mass fractions were detected by size exclusion chromatography yielding weight-average molecular weights of 4,100 and 2.6 kDa. Protein content varies between 2-3.6% (w/w). The exopolysaccharide is different in the nature of the glycosidic linkage, composition of monosaccharides, protein content, and weight-average molecular weight compared to the well-known polysaccharopeptide (PSP) and polysaccharopeptide Krestin (PSK).
polysaccharide, viscosity, molecular weight, structure analysis, Trametes versicolor, bioreactor cultivation
Structure type: structural motif or average structure ; 4100000
Location inside paper: fig.5, p.832
Compound class: glucan
Contained glycoepitopes: IEDB_1397514,IEDB_141806,IEDB_142488,IEDB_146664,IEDB_153543,IEDB_158555,IEDB_161166,IEDB_241101,IEDB_558869,IEDB_857743,IEDB_983931,SB_192
Methods: 13C NMR, 1H NMR, NMR-2D, IR, partial acid hydrolysis, GC-MS, TLC, enzymatic hydrolysis, ESI-MS, acid hydrolysis, FTIR, methanolysis, HPSEC, viscosity measurement, extraction, NaBH4 reduction, SEC, reduction with NaBH4, protease treatment, HMBC, COSY, trimethylsilylation, TFA hedrolysis, sulphuric acid hydrolysis
Enzymes that release or process the structure: β-glucanase
Related record ID(s): 46681
NCBI Taxonomy refs (TaxIDs): 5325Reference(s) to other database(s): GTC:G66305IS
Show glycosyltransferases
NMR conditions: in DMSO-d6 at 353(C) K
[as TSV]
13C NMR data: present in publication
|
There is only one chemically distinct structure:
Found 
report error
(previously named: Coriolus versicolor ATCC 200801)