Taxonomic group: fungi / Basidiomycota
(Phylum: Basidiomycota)
Organ / tissue: mycelium
Publication DOI: 10.1016/j.carbpol.2010.09.057Journal NLM ID: 8307156Publisher: Elsevier
Correspondence: Zhang L <lnzhang
public.wh.hb.cn>
Institutions: Department of Chemistry, Wuhan University, Wuhan, China, College of Food Science and Technology, HuaZhong Agriculture University, Wuhan, China
A water-insoluble (1→3)-α-D-glucan from Poria cocos mycelia was fractionated, followed by phosphorylation with H3PO4 in LiCl/Me2SO containing urea to synthesize water-soluble phosphated derivatives. Their structures and chain conformations were investigated by FTIR, 31P NMR, SEC-LLS and viscometry. The Mark-Houwink equation for the phosphated derivative in 0.15 M aqueous NaCl at 30 °C was established to be [η]=2.87*10^-3 Mw 0.86±0.02. On the basis of conformational parameters calculated from wormlike cylinder model, the phosphated derivative existed as a semi-stiff chain in aqueous solution. Compared with unphosphated glucan, water-solubility and chain stiffness of the phosphated derivative increased, as a result of the introduction of phosphate group on main chain. All the phosphated derivatives exhibited significantly stronger anti-tumor activities than that of the unphosphated one, suggesting the effects of solubility and expanded chain conformation on improvement of the anti-tumor activity could not be negligible.
Chain conformation, (1→3)-α-D-glucan, anti-tumor activity, Poria cocos mycelia, phosphated derivative
Structure type: homopolymer ; 573000 (F1), 452000 (F2), 375000 (F3), 308000 (F4), 283000 (F5), 230000 (F6), 174000 (F7), 111000 (F8), 77400 (F9)
Location inside paper: Pi-PCM, F1-9, (1→3)-α-D-glucan, fig. 1
Compound class: glucan
Contained glycoepitopes: IEDB_142488,IEDB_144998,IEDB_146664,IEDB_983931,SB_192
Methods: FTIR, phosphorylation, antitumor activity assay, SEC-LLS, ICP-AES, 13P NMR, viscometry measurement
Biological activity: Pi-PCM revealed antitumor activity against Sarcoma 180 with inhibition ratio of 9.41%
Related record ID(s): 42691
NCBI Taxonomy refs (TaxIDs): 81056Reference(s) to other database(s): GTC:G02177KX
Show glycosyltransferases
There is only one chemically distinct structure:
Taxonomic group: fungi / Basidiomycota
(Phylum: Basidiomycota)
Organ / tissue: mycelium
Publication DOI: 10.1016/j.carbpol.2010.09.057Journal NLM ID: 8307156Publisher: Elsevier
Correspondence: Zhang L <lnzhang
public.wh.hb.cn>
Institutions: Department of Chemistry, Wuhan University, Wuhan, China, College of Food Science and Technology, HuaZhong Agriculture University, Wuhan, China
A water-insoluble (1→3)-α-D-glucan from Poria cocos mycelia was fractionated, followed by phosphorylation with H3PO4 in LiCl/Me2SO containing urea to synthesize water-soluble phosphated derivatives. Their structures and chain conformations were investigated by FTIR, 31P NMR, SEC-LLS and viscometry. The Mark-Houwink equation for the phosphated derivative in 0.15 M aqueous NaCl at 30 °C was established to be [η]=2.87*10^-3 Mw 0.86±0.02. On the basis of conformational parameters calculated from wormlike cylinder model, the phosphated derivative existed as a semi-stiff chain in aqueous solution. Compared with unphosphated glucan, water-solubility and chain stiffness of the phosphated derivative increased, as a result of the introduction of phosphate group on main chain. All the phosphated derivatives exhibited significantly stronger anti-tumor activities than that of the unphosphated one, suggesting the effects of solubility and expanded chain conformation on improvement of the anti-tumor activity could not be negligible.
Chain conformation, (1→3)-α-D-glucan, anti-tumor activity, Poria cocos mycelia, phosphated derivative
Structure type: structural motif or average structure ; 156000 (P1), 92900 (P2), 78700 (P3), 56700 (P4), 35000 (P5), 34500 (P6), 31400 (P7), 29800 (P8), 19600 (P9)
Location inside paper: P1-P9, fig. 1
Compound class: glucan
Contained glycoepitopes: IEDB_142488,IEDB_144998,IEDB_144999,IEDB_146664,IEDB_241118,IEDB_983931,SB_192
Methods: FTIR, phosphorylation, antitumor activity assay, SEC-LLS, ICP-AES, 13P NMR, viscometry measurement
Biological activity: fractions of phosphorilated Pi-PCM (P1-5,7,9) revealed antitumor activity against Sarcoma 180 with inhibition ratios of 62.5%, 55.7%, 54.1%, 50.8%, 34.2%, 30.6%, 24.3% respectively
Synthetic data: chemical
Comments, role: chemical modification of ID 47690; degree of phosphate group substitution in P1-9 fractions are 0.96%, 1%, 1.43%, 1.40%, 1.95%, 2.01%, 2.78%, 2.81%, 2.79% respectively
Related record ID(s): 42690
NCBI Taxonomy refs (TaxIDs): 81056Reference(s) to other database(s): GTC:G55388UO
Show glycosyltransferases
There is only one chemically distinct structure:
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(later renamed to: Wolfiporia cocos)