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Li X, Wang L, Wang Z
Structural characterization and antioxidant activity of polysaccharide from Hohenbuehelia serotina
International Journal of Biological Macromolecules 98 (2017)
59-66
Hohenbuehelia serotina
(previously named: Panellus serotinus, Panus serotinus, Pleurotus serotinus; later renamed to: Sarcomyxa serotina)
(NCBI TaxID 40488,
species name lookup)
Taxonomic group: fungi / Basidiomycota
(Phylum: Basidiomycota)
Organ / tissue: fruiting body
The structure was elucidated in this paperPublication DOI: 10.1016/j.ijbiomac.2016.12.089Journal NLM ID: 7909578Publisher: Butterworth-Heinemann
Correspondence: lixiaoyu
ysu.edu.cn
Institutions: School of Environmental and Chemical Engineering, Yanshan University, Qinhuangdao, China, School of Food Science and Engineering, Harbin Institute of Technology, Harbin, China, School of Environmental and Chemical Engineering, Yanshan University, Qinhuangdao 066004, PR China, School of Food Science and Engineering, Harbin Institute of Technology, Harbin 150090, PR China
Previous research found that the polysaccharides isolated from Hohenbuehelia serotina possessed various biological activities, such as antioxidant, immunomodulation and radioprotective effects. However, the structural information of H. serotina polysaccharides has not yet been reported. Therefore, based on the investigation of the antioxidative tracking, a novel polysaccharide named as NTHSP-A1 was isolated from H. serotina by ultrasonic-assistance extraction and anion-exchange and gel permeation chromatography approaches. Structural characterization revealed that NTHSP-A1 had an average molecular weight of 8.09 × 103 Da and composed of arabinose, mannose, glucose and galactose in a molar ratio of 4:16:28:11. The polysaccharide was semi-crystalline substance with multi-branching structure. The backbone of NTHSP-A1 was shown to contain →3,6)-α-D-Glcp-(1→, with branches substituted at C-3 of →2)-α-L-Arap-(1→, C-3 of α-D-Manp-(1→, and C-6 of →6)-β-D-Galp-(1→, respectively. This study provides a theoretical basis for the further research on the relationship between biological activity and structure of NTHSP-A1.
polysaccharide, Structural characterization, Antioxidant activity
Structure type: structural motif or average structure ; 809000
Location inside paper: NTHSP-A1, Table 2, p.64
Compound class: O-polysaccharide, glucogalactan
Contained glycoepitopes: IEDB_136044,IEDB_137472,IEDB_141794,IEDB_142488,IEDB_144998,IEDB_146664,IEDB_190606,IEDB_983931,SB_165,SB_166,SB_187,SB_192,SB_195,SB_7,SB_88
Methods: 13C NMR, 1H NMR, GC-MS, X-ray, GC, HPLC, acetylation, atomic force microscopy, methylation analysis, trifluoroacetic acid solvolysis, HPGPC, HMBC, HMQC, COSY, FT-IR, phenol-sulphuric acid method
Biological activity: NTHSP-A1 polysaccharide possesed remarkable inhibitory actions with the ABTS and hydroxyl IC50 values of 35.84 ± 0.24 and 95.08 ± 3.27 mg VCE/g polysaccharide, respectively (p < 0.01)
Comments, role: part of NTHSP-A1 polysaccharide complex stucture, which consists of successively linked (ID 43227)x (ID 43228)a (ID 43229) (ID 43227)y (ID 43228)b residues with x+y=2 and a+b=4. Published erroneous NMR chemical shifts of the O-methyl substituent were removed by CSDB staff.
Related record ID(s): 43228, 43229
NCBI Taxonomy refs (TaxIDs): 40488Reference(s) to other database(s): GTC:G88843WW
Show glycosyltransferases
NMR conditions: in D2O at 343 K
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6
6,6 Me
6 bDGalp 103.5 73.9 75.6 69.8 75.6 69.1
aDGlcp 98.8 72.1 75.6 73.9 71.1 68.2
1H NMR data:
Linkage Residue H1 H2 H3 H4 H5 H6
6,6 Me
6 bDGalp 4.7 3.7 3.6 3.9 3.9 3.7
aDGlcp 5.3 3.6 4.1 3.7 3.6 3.7
1H/13C HSQC data:
Linkage Residue C1/H1 C2/H2 C3/H3 C4/H4 C5/H5 C6/H6
6,6 Me
6 bDGalp 103.5/4.7 73.9/3.7 75.6/3.6 69.8/3.9 75.6/3.9 69.1/3.7
aDGlcp 98.8/5.3 72.1/3.6 75.6/4.1 73.9/3.7 71.1/3.6 68.2/3.7
1H NMR data:
| Linkage | Residue | H1 | H2 | H3 | H4 | H5 | H6 |
|---|
| 6,6 | Me | |
| 6 | bDGalp | 4.7 | 3.7 | 3.6 | 3.9 | 3.9 | 3.7 |
| | aDGlcp | 5.3 | 3.6 | 4.1 | 3.7 | 3.6 | 3.7 |
|
13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 |
|---|
| 6,6 | Me | |
| 6 | bDGalp | 103.5 | 73.9 | 75.6 | 69.8 | 75.6 | 69.1 |
| | aDGlcp | 98.8 | 72.1 | 75.6 | 73.9 | 71.1 | 68.2 |
|
There is only one chemically distinct structure:
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Li X, Wang L, Wang Z
Structural characterization and antioxidant activity of polysaccharide from Hohenbuehelia serotina
International Journal of Biological Macromolecules 98 (2017)
59-66
Hohenbuehelia serotina
(previously named: Panellus serotinus, Panus serotinus, Pleurotus serotinus; later renamed to: Sarcomyxa serotina)
(NCBI TaxID 40488,
species name lookup)
Taxonomic group: fungi / Basidiomycota
(Phylum: Basidiomycota)
Organ / tissue: fruiting body
The structure was elucidated in this paperPublication DOI: 10.1016/j.ijbiomac.2016.12.089Journal NLM ID: 7909578Publisher: Butterworth-Heinemann
Correspondence: lixiaoyu
ysu.edu.cn
Institutions: School of Environmental and Chemical Engineering, Yanshan University, Qinhuangdao, China, School of Food Science and Engineering, Harbin Institute of Technology, Harbin, China, School of Environmental and Chemical Engineering, Yanshan University, Qinhuangdao 066004, PR China, School of Food Science and Engineering, Harbin Institute of Technology, Harbin 150090, PR China
Previous research found that the polysaccharides isolated from Hohenbuehelia serotina possessed various biological activities, such as antioxidant, immunomodulation and radioprotective effects. However, the structural information of H. serotina polysaccharides has not yet been reported. Therefore, based on the investigation of the antioxidative tracking, a novel polysaccharide named as NTHSP-A1 was isolated from H. serotina by ultrasonic-assistance extraction and anion-exchange and gel permeation chromatography approaches. Structural characterization revealed that NTHSP-A1 had an average molecular weight of 8.09 × 103 Da and composed of arabinose, mannose, glucose and galactose in a molar ratio of 4:16:28:11. The polysaccharide was semi-crystalline substance with multi-branching structure. The backbone of NTHSP-A1 was shown to contain →3,6)-α-D-Glcp-(1→, with branches substituted at C-3 of →2)-α-L-Arap-(1→, C-3 of α-D-Manp-(1→, and C-6 of →6)-β-D-Galp-(1→, respectively. This study provides a theoretical basis for the further research on the relationship between biological activity and structure of NTHSP-A1.
polysaccharide, Structural characterization, Antioxidant activity
Structure type: structural motif or average structure ; 809000
Location inside paper: NTHSP-A1, Table 2, p.64
Compound class: O-polysaccharide, glucomannan
Contained glycoepitopes: IEDB_130701,IEDB_142488,IEDB_144983,IEDB_144998,IEDB_146664,IEDB_152206,IEDB_158538,IEDB_983930,IEDB_983931,SB_192,SB_44,SB_67,SB_72
Methods: 13C NMR, 1H NMR, GC-MS, X-ray, GC, HPLC, acetylation, atomic force microscopy, methylation analysis, trifluoroacetic acid solvolysis, HPGPC, HMBC, HMQC, COSY, FT-IR, phenol-sulphuric acid method
Biological activity: NTHSP-A1 polysaccharide possesed remarkable inhibitory actions with the ABTS and hydroxyl IC50 values of 35.84 ± 0.24 and 95.08 ± 3.27 mg VCE/g polysaccharide, respectively (p < 0.01)
Comments, role: part of NTHSP-A1 polysaccharide complex stucture, which consists of repeating unit of successively linked [(ID 43227)x (ID 43228)a (ID 43229) (ID 43227)y (ID 43228)b] with x+y=2 and a+b=4
Related record ID(s): 43227, 43229
NCBI Taxonomy refs (TaxIDs): 40488Reference(s) to other database(s): GTC:G90061QA
Show glycosyltransferases
NMR conditions: in D2O at 343 K
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6
3 aDManp 102.1 70.1 72.1 67.7 73.6 61.8
aDGlcp 98.8 72.1 75.6 73.9 71.1 68.2
1H NMR data:
Linkage Residue H1 H2 H3 H4 H5 H6
3 aDManp 5.1 4.1 3.9 3.6 3.9 3.7
aDGlcp 5.3 3.6 4.1 3.7 3.6 3.7
1H/13C HSQC data:
Linkage Residue C1/H1 C2/H2 C3/H3 C4/H4 C5/H5 C6/H6
3 aDManp 102.1/5.1 70.1/4.1 72.1/3.9 67.7/3.6 73.6/3.9 61.8/3.7
aDGlcp 98.8/5.3 72.1/3.6 75.6/4.1 73.9/3.7 71.1/3.6 68.2/3.7
1H NMR data:
| Linkage | Residue | H1 | H2 | H3 | H4 | H5 | H6 |
|---|
| 3 | aDManp | 5.1 | 4.1 | 3.9 | 3.6 | 3.9 | 3.7 |
| | aDGlcp | 5.3 | 3.6 | 4.1 | 3.7 | 3.6 | 3.7 |
|
13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 |
|---|
| 3 | aDManp | 102.1 | 70.1 | 72.1 | 67.7 | 73.6 | 61.8 |
| | aDGlcp | 98.8 | 72.1 | 75.6 | 73.9 | 71.1 | 68.2 |
|
There is only one chemically distinct structure:
Expand this record
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Li X, Wang L, Wang Z
Structural characterization and antioxidant activity of polysaccharide from Hohenbuehelia serotina
International Journal of Biological Macromolecules 98 (2017)
59-66
Hohenbuehelia serotina
(previously named: Panellus serotinus, Panus serotinus, Pleurotus serotinus; later renamed to: Sarcomyxa serotina)
(NCBI TaxID 40488,
species name lookup)
Taxonomic group: fungi / Basidiomycota
(Phylum: Basidiomycota)
Organ / tissue: fruiting body
The structure was elucidated in this paperPublication DOI: 10.1016/j.ijbiomac.2016.12.089Journal NLM ID: 7909578Publisher: Butterworth-Heinemann
Correspondence: lixiaoyu
ysu.edu.cn
Institutions: School of Environmental and Chemical Engineering, Yanshan University, Qinhuangdao, China, School of Food Science and Engineering, Harbin Institute of Technology, Harbin, China, School of Environmental and Chemical Engineering, Yanshan University, Qinhuangdao 066004, PR China, School of Food Science and Engineering, Harbin Institute of Technology, Harbin 150090, PR China
Previous research found that the polysaccharides isolated from Hohenbuehelia serotina possessed various biological activities, such as antioxidant, immunomodulation and radioprotective effects. However, the structural information of H. serotina polysaccharides has not yet been reported. Therefore, based on the investigation of the antioxidative tracking, a novel polysaccharide named as NTHSP-A1 was isolated from H. serotina by ultrasonic-assistance extraction and anion-exchange and gel permeation chromatography approaches. Structural characterization revealed that NTHSP-A1 had an average molecular weight of 8.09 × 103 Da and composed of arabinose, mannose, glucose and galactose in a molar ratio of 4:16:28:11. The polysaccharide was semi-crystalline substance with multi-branching structure. The backbone of NTHSP-A1 was shown to contain →3,6)-α-D-Glcp-(1→, with branches substituted at C-3 of →2)-α-L-Arap-(1→, C-3 of α-D-Manp-(1→, and C-6 of →6)-β-D-Galp-(1→, respectively. This study provides a theoretical basis for the further research on the relationship between biological activity and structure of NTHSP-A1.
polysaccharide, Structural characterization, Antioxidant activity
Structure type: structural motif or average structure ; 809000
Location inside paper: NTHSP-A1, Table 2, p.64
Compound class: O-polysaccharide, arabinoglucan
Contained glycoepitopes: IEDB_142488,IEDB_144998,IEDB_146664,IEDB_158538,IEDB_983931,SB_192
Methods: 13C NMR, 1H NMR, GC-MS, X-ray, GC, HPLC, acetylation, atomic force microscopy, methylation analysis, trifluoroacetic acid solvolysis, HPGPC, HMBC, HMQC, COSY, FT-IR, phenol-sulphuric acid method
Biological activity: NTHSP-A1 polysaccharide possesed remarkable inhibitory actions with the ABTS and hydroxyl IC50 values of 35.84 ± 0.24 and 95.08 ± 3.27 mg VCE/g polysaccharide, respectively (p < 0.01)
Comments, role: part of NTHSP-A1 polysaccharide complex stucture, which consists of repeating unit of successively linked [(ID 43227)x (ID 43228)a (ID 43229) (ID 43227)y (ID 43228)b] with x+y=2 and a+b=4. Published erroneous NMR chemical shifts of the O-methyl substituent were removed by CSDB staff.
Related record ID(s): 43227, 43228
NCBI Taxonomy refs (TaxIDs): 40488Reference(s) to other database(s): GTC:G83229GM
Show glycosyltransferases
NMR conditions: in D2O at 343 K
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6
3,2 Me
3 aLArap 102.7 78.3 69.0 68.2 69.2
aDGlcp 98.8 72.1 75.6 73.9 71.1 68.2
1H NMR data:
Linkage Residue H1 H2 H3 H4 H5 H6
3,2 Me
3 aLArap 5.3 3.9 4.1 3.7 3.7
aDGlcp 5.3 3.6 4.1 3.7 3.6 3.7
1H/13C HSQC data:
Linkage Residue C1/H1 C2/H2 C3/H3 C4/H4 C5/H5 C6/H6
3,2 Me
3 aLArap 102.7/5.3 78.3/3.9 69.0/4.1 68.2/3.7 69.2/3.7
aDGlcp 98.8/5.3 72.1/3.6 75.6/4.1 73.9/3.7 71.1/3.6 68.2/3.7
1H NMR data:
| Linkage | Residue | H1 | H2 | H3 | H4 | H5 | H6 |
|---|
| 3,2 | Me | |
| 3 | aLArap | 5.3 | 3.9 | 4.1 | 3.7 | 3.7 | |
| | aDGlcp | 5.3 | 3.6 | 4.1 | 3.7 | 3.6 | 3.7 |
|
13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 |
|---|
| 3,2 | Me | |
| 3 | aLArap | 102.7 | 78.3 | 69.0 | 68.2 | 69.2 | |
| | aDGlcp | 98.8 | 72.1 | 75.6 | 73.9 | 71.1 | 68.2 |
|
There is only one chemically distinct structure:
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