Taxonomic group: fungi / Ascomycota
(Phylum: Ascomycota)
Publication DOI: 10.1016/j.procbio.2010.11.004Journal NLM ID: 9211419Publisher: Barking, Essex: Elsevier Applied Science
Correspondence: Liu DQ <dongqiwh
yahoo.com>
Institutions: Center for Microbiological Engineering, School of Environmental Science and Engineering, Huazhong University of Science and Technology, Wuhan, China
Pullulan is a water-soluble extracelluler polysaccharide produced aerobically by fungus Aureobasidium pullulans and some other species. Current work focused on developing an A. pullulans strain with enhanced pullulan productivity and raw material utilization ratio using a new method for genome shuffling of A. pullulans N3.387. After the third cycle of the protoplast fusion, a mutant, designated as F3-2, producing 179.7% (20.7 g/L) more pullulan than the wild-type strain was obtained. Furthermore, the raw material utilization ratio of F3-2 was determined as 97.9%, which was 29.0% higher than the wild-type strain. Genetic stability analysis of F3-2 showed that ranges of the pullulan production and the raw material utilization ratio of the generation were 0.13 g/L and 1.93%, respectively, suggesting that F3-2 was genetically stable. Elucidation of this new genome shuffling process is also interesting for enhancing production of extracellular polysaccharide in both A. pullulans and other fungus.
pullulan, Aureobasidium pullulans, genetic stability, genome shuffling, inactive protoplast fusion, protoplast formation
Structure type: polymer chemical repeating unit
Location inside paper: p.792
Trivial name: pullulan
Compound class: EPS, O-polysaccharide, cell wall polysaccharide, glucan, polysaccharide
Contained glycoepitopes: IEDB_140629,IEDB_142488,IEDB_144998,IEDB_146664,IEDB_420419,IEDB_420420,IEDB_420421,IEDB_857742,IEDB_983931,SB_192
Methods: DNA techniques, UV, extraction, cell growth, anthracenone-vitriol method, DNS method
Related record ID(s): 43437, 49953, 49977, 49978, 49979, 49995, 49999, 50000, 50002, 50007, 50014, 50071, 50081, 50082, 50083, 50111, 50113
NCBI Taxonomy refs (TaxIDs): 5580Reference(s) to other database(s): GTC:G71532WE, CCSD:
45938, CBank-STR:4859, GenDB:KY767023; GenDB:KY767024
Show glycosyltransferases
There is only one chemically distinct structure:
Taxonomic group: bacteria / Actinobacteria
(Phylum: Actinobacteria)
Associated disease: infection due to Mycobacterium bovis [ICD11:
XN8AB 
];
infection due to Mycobacterium tuberculosis [ICD11:
XN1N2 ]
The structure was elucidated in this paperNCBI PubMed ID: 26005149Publication DOI: 10.1016/j.carbpol.2015.04.025Journal NLM ID: 8307156Publisher: Elsevier
Correspondence: liushao999
hotmail.com
Institutions: Department of Pharmacy, Xiangya Hospital, Changsha, China, School of Pharmacy, Central South University, Changsha, China, Jiuzhitang Co., Ltd, Changsha, China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, China, Tumor Institute of Central South University, Changsha, China
A water-soluble glucan, BCG-PASW, with a molecular weight of 21 kDa, was separated from polysaccharide nucleic acid fraction of Bacillus Calmette Guerin (BCG-PSN) using DEAE-52 cellulose and Sephadex G-200 chromatography. Based on gas chromatography-mass spectrometry (GC-MS), fourier transform infrared (FT-IR) spectra, 1D and 2D nuclear magnetic resonance (NMR) spectroscopy techniques (COSY, HSQC and HMBC), BCG-PASW was found to be an α-D-glucan composed of α-D-(1→4)-linked glucopyranosyl residues, with branches at O-6 consisting of non-reducing terminal α-D-Glcp approximately every eight residues. In vitro antitumor activity by MTS method, its sulfated derivative with a substitution degree of 0.59, could inhibite C666-1 nasopharyngeal carcinoma cells growth significantly. The results indicated that the sulfate content play a decisive role in the bioactivities of the polysaccharides
characterization, D-mannose, purification, N, antitumor, BCG-PSN, chlorosulfonic acid, methoxyamine, O-bis(trime.silyl)trifluoroacetamide, sulfated derivative, D-arabinose, D-glucopyranose, D-ribose
Structure type: structural motif or average structure ; 21000
Location inside paper: p. 142, structure, left column, paragraph 2, Table 2
Compound class: glucan, polysaccharide
Contained glycoepitopes: IEDB_142488,IEDB_144998,IEDB_146664,IEDB_983931,SB_192
Methods: 13C NMR, 1H NMR, NMR-2D, methylation, IR, GC-MS, acid hydrolysis, acetylation, methylation analysis, reduction, column chromatography, dialysis, HPGPC, precipitation, derivatization, antitumor activity assay, centrifugation, anthracenone-vitriol method
Biological activity: polysaccharide have antitumor activity against C666-1 nasopharyngeal carcinoma cells
NCBI Taxonomy refs (TaxIDs): 33892Reference(s) to other database(s): GTC:G39749TV
Show glycosyltransferases
NMR conditions: in D2O at 298 K
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6
4 aDGlcp 102.44 74.46 76.19 79.44 73.75 63.25
6 aDGlcp 98.61 ? ? ? ? ?
aDGlcp 101.02 ? ? 78.31 ? 72.24
1H NMR data:
Linkage Residue H1 H2 H3 H4 H5 H6
4 aDGlcp 5.42 3.71 3.97 3.63 3.83 3.86
6 aDGlcp 5.05 ? ? ? ? ?
aDGlcp 5.13 ? ? ? ? ?
1H/13C HSQC data:
Linkage Residue C1/H1 C2/H2 C3/H3 C4/H4 C5/H5 C6/H6
4 aDGlcp 102.44/5.42 74.46/3.71 76.19/3.97 79.44/3.63 73.75/3.83 63.25/3.86
6 aDGlcp 98.61/5.05 ?/? ?/? ?/? ?/? ?/?
aDGlcp 101.02/5.13 ?/? ?/? 78.31/? ?/? 72.24/?
1H NMR data:
| Linkage | Residue | H1 | H2 | H3 | H4 | H5 | H6 |
|---|
| 4 | aDGlcp | 5.42 | 3.71 | 3.97 | 3.63 | 3.83 | 3.86 |
| 6 | aDGlcp | 5.05 | ? | ? | ? | ? | ? |
| | aDGlcp | 5.13 | ? | ? | ? | ? | ? |
|
13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 |
|---|
| 4 | aDGlcp | 102.44 | 74.46 | 76.19 | 79.44 | 73.75 | 63.25 |
| 6 | aDGlcp | 98.61 | ? | ? | ? | ? | ? |
| | aDGlcp | 101.02 | ? | ? | 78.31 | ? | 72.24 |
|
The spectrum also has 8 signals at unknown positions (not plotted). |
There is only one chemically distinct structure:
Found 
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