Taxonomic group: fungi / Basidiomycota
(Phylum: Basidiomycota)
Organ / tissue: fruiting body
The structure was elucidated in this paperNCBI PubMed ID: 21129732Publication DOI: 10.1016/j.carres.2010.10.026Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: Islam SS <sirajul_1999
yahoo.com>
Institutions: Department of Chemistry and Chemical Technology, Vidyasagar University, Midnapore, India, Department of Biotechnology, Indian Institute of Technology (IIT) Kharagpur, Kharagpur, India, Sangri-la Mushroom, Mohitnagar, Jalpaiguri, India
A water soluble polysaccharide isolated from the hot aqueous extract of Pleurotusostreatus cultivar was found to contain d-glucose and d-galactose in a molar ratio of nearly 7:1. Structural investigation was carried out using acid hydrolysis, methylation analysis, periodate oxidation, Smith degradation, and NMR studies ((1)H, (13)C, DEPT-135, TOCSY, DQF-COSY, NOESY, ROESY, HMQC, and HMBC). On the basis of the above mentioned experiments the structure of the repeating unit of the polysaccharide was established as: -3)bDGlcp(1-6)[aDGalp(1-3)]bDGlcp(1-6)bDGlcp(1-3)bDGlcp(1-6)[aDGlcp(1-3)]bDGlcp(1-6)bDGlcp(1-. This heteroglycan stimulates macrophages, splenocytes, and thymocytes.
structure, polysaccharide, NMR spectroscopy, Immunoactivation, Pleurotus ostreatus cultivar
Structure type: polymer chemical repeating unit
Location inside paper: p. 368, Table I
Compound class: O-polysaccharide
Contained glycoepitopes: IEDB_135614,IEDB_136906,IEDB_137472,IEDB_141794,IEDB_141806,IEDB_142488,IEDB_144998,IEDB_146664,IEDB_151528,IEDB_153543,IEDB_153755,IEDB_190606,IEDB_241101,IEDB_983931,SB_192,SB_7
Methods: 13C NMR, 1H NMR, NMR-2D, GLC-MS, acid hydrolysis, Smith degradation, GPC, periodate oxidation, optical rotation measurement, methylation analysis, DEPT, splenocyte proliferation assay, thymocyte proliferation assay, macrophage activity
Biological activity: Proliferator of splenocytes and thymocytes, activate macrophages.
Comments, role: 1H NMR is referenced to HDO at 4.76, 13C NMR is referenced to acetone at 31.05.
Related record ID(s): 43608, 50109
NCBI Taxonomy refs (TaxIDs): 5322Reference(s) to other database(s): GTC:G17243GP
Show glycosyltransferases
NMR conditions: in D2O at 300 K
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6
6,6,3,6,6 bDGlcp 103.3 73.3 84.8 68.9 75.2 61.1
6,6,3,6,3 aDGalp 99.7 67.2 69.9 70.5 70.8 61.4
6,6,3,6 bDGlcp 103.2 73.8 84.9 68.6 75.3 69.3
6,6,3 bDGlcp 103.1 73.5 76.7 70.9 75.3 69.2
6,6 bDGlcp 103.3 73.3 84.8 68.9 75.2 61.1
6,3 aDGlcp 98.6 69.2 73.2 69.9 70.8 61.1
6 bDGlcp 103.2 73.8 84.9 68.6 75.3 69.3
bDGlcp 103.1 73.5 76.7 70.9 75.3 69.2
1H NMR data:
Linkage Residue H1 H2 H3 H4 H5 H6
6,6,3,6,6 bDGlcp 4.51 3.51 3.71 3.47 3.58 3.71-3.90
6,6,3,6,3 aDGalp 5.11 3.91 3.99 3.88 4.08 3.69-3.71
6,6,3,6 bDGlcp 4.47 3.31 3.72 3.58 3.62 3.85-4.19
6,6,3 bDGlcp 4.49 3.30 3.43 3.38 3.46 3.91-4.20
6,6 bDGlcp 4.51 3.51 3.71 3.47 3.58 3.71-3.90
6,3 aDGlcp 4.98 3.85 3.65 3.48 3.84 3.88-3.91
6 bDGlcp 4.47 3.31 3.72 3.58 3.62 3.85-4.19
bDGlcp 4.49 3.30 3.43 3.38 3.46 3.91-4.20
1H/13C HSQC data:
Linkage Residue C1/H1 C2/H2 C3/H3 C4/H4 C5/H5 C6/H6
6,6,3,6,6 bDGlcp 103.3/4.51 73.3/3.51 84.8/3.71 68.9/3.47 75.2/3.58 61.1/3.71-3.90
6,6,3,6,3 aDGalp 99.7/5.11 67.2/3.91 69.9/3.99 70.5/3.88 70.8/4.08 61.4/3.69-3.71
6,6,3,6 bDGlcp 103.2/4.47 73.8/3.31 84.9/3.72 68.6/3.58 75.3/3.62 69.3/3.85-4.19
6,6,3 bDGlcp 103.1/4.49 73.5/3.30 76.7/3.43 70.9/3.38 75.3/3.46 69.2/3.91-4.20
6,6 bDGlcp 103.3/4.51 73.3/3.51 84.8/3.71 68.9/3.47 75.2/3.58 61.1/3.71-3.90
6,3 aDGlcp 98.6/4.98 69.2/3.85 73.2/3.65 69.9/3.48 70.8/3.84 61.1/3.88-3.91
6 bDGlcp 103.2/4.47 73.8/3.31 84.9/3.72 68.6/3.58 75.3/3.62 69.3/3.85-4.19
bDGlcp 103.1/4.49 73.5/3.30 76.7/3.43 70.9/3.38 75.3/3.46 69.2/3.91-4.20
1H NMR data:
| Linkage | Residue | H1 | H2 | H3 | H4 | H5 | H6 |
|---|
| 6,6,3,6,6 | bDGlcp | 4.51 | 3.51 | 3.71 | 3.47 | 3.58 | 3.71
3.90 |
| 6,6,3,6,3 | aDGalp | 5.11 | 3.91 | 3.99 | 3.88 | 4.08 | 3.69
3.71 |
| 6,6,3,6 | bDGlcp | 4.47 | 3.31 | 3.72 | 3.58 | 3.62 | 3.85
4.19 |
| 6,6,3 | bDGlcp | 4.49 | 3.30 | 3.43 | 3.38 | 3.46 | 3.91
4.20 |
| 6,6 | bDGlcp | 4.51 | 3.51 | 3.71 | 3.47 | 3.58 | 3.71
3.90 |
| 6,3 | aDGlcp | 4.98 | 3.85 | 3.65 | 3.48 | 3.84 | 3.88
3.91 |
| 6 | bDGlcp | 4.47 | 3.31 | 3.72 | 3.58 | 3.62 | 3.85
4.19 |
| | bDGlcp | 4.49 | 3.30 | 3.43 | 3.38 | 3.46 | 3.91
4.20 |
|
13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 |
|---|
| 6,6,3,6,6 | bDGlcp | 103.3 | 73.3 | 84.8 | 68.9 | 75.2 | 61.1 |
| 6,6,3,6,3 | aDGalp | 99.7 | 67.2 | 69.9 | 70.5 | 70.8 | 61.4 |
| 6,6,3,6 | bDGlcp | 103.2 | 73.8 | 84.9 | 68.6 | 75.3 | 69.3 |
| 6,6,3 | bDGlcp | 103.1 | 73.5 | 76.7 | 70.9 | 75.3 | 69.2 |
| 6,6 | bDGlcp | 103.3 | 73.3 | 84.8 | 68.9 | 75.2 | 61.1 |
| 6,3 | aDGlcp | 98.6 | 69.2 | 73.2 | 69.9 | 70.8 | 61.1 |
| 6 | bDGlcp | 103.2 | 73.8 | 84.9 | 68.6 | 75.3 | 69.3 |
| | bDGlcp | 103.1 | 73.5 | 76.7 | 70.9 | 75.3 | 69.2 |
|
There is only one chemically distinct structure:
Taxonomic group: fungi / Basidiomycota
(Phylum: Basidiomycota)
Organ / tissue: fruiting body
The structure was elucidated in this paperNCBI PubMed ID: 21129732Publication DOI: 10.1016/j.carres.2010.10.026Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: Islam SS <sirajul_1999
yahoo.com>
Institutions: Department of Chemistry and Chemical Technology, Vidyasagar University, Midnapore, India, Department of Biotechnology, Indian Institute of Technology (IIT) Kharagpur, Kharagpur, India, Sangri-la Mushroom, Mohitnagar, Jalpaiguri, India
A water soluble polysaccharide isolated from the hot aqueous extract of Pleurotusostreatus cultivar was found to contain d-glucose and d-galactose in a molar ratio of nearly 7:1. Structural investigation was carried out using acid hydrolysis, methylation analysis, periodate oxidation, Smith degradation, and NMR studies ((1)H, (13)C, DEPT-135, TOCSY, DQF-COSY, NOESY, ROESY, HMQC, and HMBC). On the basis of the above mentioned experiments the structure of the repeating unit of the polysaccharide was established as: -3)bDGlcp(1-6)[aDGalp(1-3)]bDGlcp(1-6)bDGlcp(1-3)bDGlcp(1-6)[aDGlcp(1-3)]bDGlcp(1-6)bDGlcp(1-. This heteroglycan stimulates macrophages, splenocytes, and thymocytes.
structure, polysaccharide, NMR spectroscopy, Immunoactivation, Pleurotus ostreatus cultivar
Structure type: fragment of a bigger structure
Location inside paper: p. 370, Table IV
Contained glycoepitopes: IEDB_141806,IEDB_142488,IEDB_146664,IEDB_241101,IEDB_983931,SB_192
Methods: 13C NMR, 1H NMR, NMR-2D, GLC-MS, acid hydrolysis, Smith degradation, GPC, periodate oxidation, optical rotation measurement, methylation analysis, DEPT, splenocyte proliferation assay, thymocyte proliferation assay, macrophage activity
Biological activity: Proliferator of splenocytes and thymocytes, activate macrophages. Maximum value was observed at 25 lg/mL.
Comments, role: This structure is the product of the Smith degraded of water soluble polysaccharide from Pleurotus ostreatus. 1H NMR is referenced to HDO at 4.76, 13C NMR is referenced to acetone at 31.05.
Related record ID(s): 43607
NCBI Taxonomy refs (TaxIDs): 5322Reference(s) to other database(s): GTC:G18208RB
Show glycosyltransferases
NMR conditions: in D2O at 300 K
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6
1,6 bDGlcp 103.1 73.8 76.3 69.7 77.0 61.4
1 bDGlcp 102.9 73.2 75.2 70.2 76.6 69.0
x?Gro 66.8 72.4 62.9
1H NMR data:
Linkage Residue H1 H2 H3 H4 H5 H6
1,6 bDGlcp 4.51 3.49 3.71 3.53 3.65 3.73-3.88
1 bDGlcp 4.47 3.41 3.68 3.53 3.65 3.82-4.17
x?Gro 3.90-4.23 3.74 3.52-3.67
1H/13C HSQC data:
Linkage Residue C1/H1 C2/H2 C3/H3 C4/H4 C5/H5 C6/H6
1,6 bDGlcp 103.1/4.51 73.8/3.49 76.3/3.71 69.7/3.53 77.0/3.65 61.4/3.73-3.88
1 bDGlcp 102.9/4.47 73.2/3.41 75.2/3.68 70.2/3.53 76.6/3.65 69.0/3.82-4.17
x?Gro 66.8/3.90-4.23 72.4/3.74 62.9/3.52-3.67
1H NMR data:
| Linkage | Residue | H1 | H2 | H3 | H4 | H5 | H6 |
|---|
| 1,6 | bDGlcp | 4.51 | 3.49 | 3.71 | 3.53 | 3.65 | 3.73
3.88 |
| 1 | bDGlcp | 4.47 | 3.41 | 3.68 | 3.53 | 3.65 | 3.82
4.17 |
| | x?Gro | 3.90
4.23 | 3.74 | 3.52
3.67 | |
|
13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 |
|---|
| 1,6 | bDGlcp | 103.1 | 73.8 | 76.3 | 69.7 | 77.0 | 61.4 |
| 1 | bDGlcp | 102.9 | 73.2 | 75.2 | 70.2 | 76.6 | 69.0 |
| | x?Gro | 66.8 | 72.4 | 62.9 | |
|
There is only one chemically distinct structure:
report error
Found 2 records.
Displayed records from 1 to 2
