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Taxonomic group: fungi / Basidiomycota (Phylum: Basidiomycota)
Organ / tissue: fruiting body

The structure was elucidated in this paper
NCBI PubMed ID: 20430053
Publication DOI: 10.1016/j.ijbiomac.2010.04.010
Journal NLM ID: 7909578
Publisher: Butterworth-Heinemann
Correspondence: Zhao J <biostart8083

yahoo.cn>
Institutions: Key Laboratory of Biological Resource and Ecological Environment of the Ministry of Education, College of Life Sciences, Sichuan University, Chengdu, China, Key Laboratory of Sichuan Academy of Medical Sciences, Sichuan Provincial People’s Hospital, Chengdu, China

In this study, structural features of Tricholoma matsutake polysaccharide (TMP-A) were investigated by a combination of infrared (IR) spectra, gas chromatography-mass spectrometry (GC-MS), nuclear magnetic resonance (NMR) spectroscopy. The results indicated that TMP-A had a backbone of 1,4-β-D-glucopyranose residue which branches at O-6 based on the experimental results. The branches were mainly composed of an (1→3)-α-d-galactopyranose residue, and terminated with α-d-xylopyranose residue. The antioxidant activity of TMP-A was evaluated with several biochemical methods, including DPPH- radical scavenging, hydrogen peroxide scavenging, superoxide anion radical scavenging. The results indicated that TMP-A showed strong antioxidant. In the in vitro antioxidant assay by MTT method, TMP-A could attenuate PC12 cell damage significantly caused by hydrogen peroxide.

polysaccharide structure, antioxidant assay, Tricholoma matsutake

Structure type: structural motif or average structure ; 88900
Location inside paper: Fig.3, table 1, TMP-A
Compound class: O-polysaccharide, xylogalactoglucan
Contained glycoepitopes: IEDB_114701,IEDB_136906,IEDB_137472,IEDB_140529,IEDB_141794,IEDB_142488,IEDB_146664,IEDB_151528,IEDB_167069,IEDB_190606,IEDB_983931,SB_192,SB_7

Methods: 13C NMR, 1H NMR, methylation, IR, GC-MS, TLC, acid hydrolysis, UV, extraction, column chromatography, antioxidant activities, HPGPC, phenol-sulfuric acid assay, radical scavenging assay, derivatization
Biological activity: TMP-A possesses strong free radical scavenging activity and could significantly attenuate PC12 cell damage caused by hydrogen peroxide
Comments, role: 13C NMR spectra were removed by CSDB staff due to multiple errors in the published NMR assignment

Related record ID(s): 43890
NCBI Taxonomy refs (TaxIDs): 40145
Reference(s) to other database(s): GTC:G25814WM
Show glycosyltransferases

NMR conditions: in D2O [as TSV]

1H NMR data:
missing...

¹³C NMR data: present in publication with incorrect assignment

There is only one chemically distinct structure:

[*]O[C@@H]1[C@@H](CO)O[C@@H](O[C@@H]2[C@@H](CO)O[C@@H](O[C@@H]3[C@@H](CO)O[C@@H](O[C@@H]4[C@@H](CO)O[C@@H](O[C@@H]5[C@@H](CO)O[C@@H](O[C@@H]6[C@@H](CO)O[C@@H](O[C@@H]7[C@@H](CO)O[C@@H](O[C@@H]8[C@@H](CO[C@H]9O[C@H](CO)[C@H](O)[C@H](O[C@H]%10OC[C@@H](O)[C@H](O)[C@H]%10O)[C@H]9O)O[C@@H]([*])[C@H](O)[C@H]8O)[C@H](O)[C@H]7O)[C@H](O)[C@H]6O)[C@H](O)[C@H]5O)[C@H](O)[C@H]4O)[C@H](O)[C@H]3O)[C@H](O)[C@H]2O)[C@H](O)[C@H]1O

SVG MW SMILES 3D mol Ionize

Taxonomic group: fungi / Basidiomycota (Phylum: Basidiomycota)
Organ / tissue: fruiting body

The structure was elucidated in this paper
Publication DOI: 10.1016/j.carbpol.2010.04.010
Journal NLM ID: 8307156
Publisher: Elsevier
Correspondence: zj804

163.com
Institutions: Key Laboratory of Biological Resource and Ecological Environment of the Ministry of Education, College of Life Sciences, Sichuan University, Chengdu, China, Key Laboratory of Sichuan Academy of Medical Sciences, Sichuan Provincial People’s Hospital, Chengdu, China

A novel heteropolysaccharide was isolated from the fruiting bodies of Tricholoma matsutake through DEAE-cellulose column and Sephadex G-100 column. The T. matsutake polysaccharide (TMP-A) had a molecular weight of 88900 Da and was mainly composed of 4-D-Glc:4,6-D-Glc:3-D-Gal:T-D-Xyl and with the ratio of 7:1:1:1. The structural features of TMP-A were investigated by a combination of total hydrolysis, methylation analysis, gas chromatography-mass spectrometry (GC-MS), scanning electron microscope (SEM), infrared (IR) spectra, nuclear magnetic resonance (NMR) spectroscopy and dynamical analysis of the atomic force microscope (AFM) studies. The results indicated that TMP-A had a backbone of 1,4-β-glucopyranos which branches at O-6 composed of an (1→3)-α-galactopyranose residue and terminated with α-xylopyranose residue. Chain conformation study showed that the polysaccharide took random coil compact conformation.

polysaccharide structure, Tricholoma matsutake, monosaccharide component, GC–MS, 13C and 1H NMR

Structure type: structural motif or average structure ; 88900
Location inside paper: fig. 4, Fig.5, table 1, TMP-A
Compound class: O-polysaccharide, xylogalactoglucan
Contained glycoepitopes: IEDB_114701,IEDB_136906,IEDB_137472,IEDB_140529,IEDB_141794,IEDB_142488,IEDB_146664,IEDB_151528,IEDB_167069,IEDB_190606,IEDB_983931,SB_192,SB_7

Methods: 13C NMR, 1H NMR, methylation, IR, GC-MS, TLC, acid hydrolysis, UV, extraction, atomic force microscopy, column chromatography, HPGPC, phenol-sulfuric acid assay, derivatization
Comments, role: 13C NMR spectra removed by CSDB staff due to multiple errors in the published NMR assignment

Related record ID(s): 43889
NCBI Taxonomy refs (TaxIDs): 40145
Reference(s) to other database(s): GTC:G25814WM
Show glycosyltransferases

NMR conditions: in D2O

¹H NMR data: present in publication

¹³C NMR data: present in publication with incorrect assignment

There is only one chemically distinct structure:

SVG MW SMILES 3D mol Ionize