Ghasemlou M, Khodaiyan F, Jahanbin K, Gharibzahedi SM, Taheri S Structural investigation and response surface optimisation for improvement of kefiran production yield from a low-cost culture medium Food Chemistry133(2) (2012)
383-389
The structure was elucidated in this paper NCBI PubMed ID:25683410 Publication DOI:10.1016/j.foodchem.2012.01.046 Journal NLM ID:7702639 Publisher: Elsevier Applied Science Publishers Correspondence: mghasemlouut.ac.ir Institutions: Department of Food Science, Engineering and Technology, Faculty of Agricultural Engineering and Technology, University of Tehran, Iran, Shahrood University of Technology, Faculty of Agriculture, School of Agricultural Engineering, Shahrood, Iran, Chemistry and Chemical Engineering Research Center of Iran, Tehran, Iran
Kefiran, a water-soluble heteropolysaccharide with molecular weight of 1350000 Da and a specific optical rotation of +64° (c 1.0, H2O), was isolated from kefir grains grown in cheese whey and further purified through DEAE-Sepharose XK26. Response surface methodology was employed to optimise the culture conditions for kefiran production from kefir grains to be lactose concentration 67 g/l, yeast extract 13 g/l, pH 5.7 and temperature 24 °C. Intrinsic viscosity was 5.84 dl/g using the Huggins extrapolation and 5.53 dl/g using the Kramer extrapolation. Monosaccharide analysis revealed that kefiran is composed of glucose (Glc) and galactose (Gal) in a relative molar ratio of 1.0:1.1. Its structural features were elucidated by a combination of FT-IR, methylation and GC-MS analysis, periodate oxidation-Smith degradation, partial acid hydrolysis and NMR spectroscopy ( 1H, 13C and HMBC). The data obtained indicated that kefiran possessed a backbone of (1→6)-linked Glc, (1→3)-linked Gal, (1→4)-linked Gal, (1→4)-linked Glc and (1→2,6)-linked Gal, with a branch attached to O-2 of Gal residues and terminated with Glc residues.
Wang Y, He P, He L, Huang Q, Cheng J, Li W, Liu Y, Wei C Structural elucidation, antioxidant and immunomodulatory activities of a novel heteropolysaccharide from cultured Paecilomyces cicadae (Miquel.) Samson Carbohydrate Polymers216 (2019)
270-281
/Variants 0/-+
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-4)-{{{-a-D-Glcp-(1-4)-}}}/n=10/-a-D-Glcp6Me-(1-6)-b-D-Galf-(1-
/Variants 0/ is:
a-D-Glcp-(1-6)-b-D-Glcp-(1-5)-
OR (exclusively)
a-D-Glcp-(1-2)-b-D-Galp-(1-5)-
OR (exclusively)
a-D-Glcp-(1-4)-a-D-Manp-(1-5)-
Taxonomic group: fungi / Ascomycota (Phylum: Ascomycota) Organ / tissue:mycelium
The structure was elucidated in this paper NCBI PubMed ID:31047067 Publication DOI:10.1016/j.carbpol.2019.03.104 Journal NLM ID:8307156 Publisher: Elsevier Correspondence: He L <kite006126.com> Institutions: Institute of Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou, China, Key Laboratory of State Forest Food Resources Utilization and Quality Control, Zhejiang Provincial Key Laboratory of Forest Food, Zhejiang Academy of Forestry, Hangzhou, China, Department of Processing, Marine Fisheries Research Institute of Zhejiang, Zhoushan, China, Department of Food Science, Rutgers University, New Brunswick, NJ, USA
The fine structure and chain conformation of a heteropolysaccharide (PCIPS3) from mycelium of Paecilomyces cicadae were investigated via the analysis of HPLC, IR, methylation, NMR spectroscopy and multiangle light scattering. It was determined to be a 22300 g/mol heteropolysaccharide primarily composed of glucose, galactose and mannose in a molar ratio of 23.8:2.1:1.0. The PCIPS3 backbone consisted of 1,4-linked α-D-Glcp and 1,4-linked 6-O-Me-α-D-Glcp residues, which were occasionally interrupted by branched β-Galf residues through 1,6-linkage. Moreover, the α (0.60) from Mark-Houwink-Sakurada (MHS) equation suggested that PCIPS3 adopted a flexible chain conformation in 0.1 mol/L NaNO3 at 25 °C. The worm-like chains model parameters for PCIPS3 were estimated as following: ML = 437 nm-1, q = 0.46 nm and 0.79 nm, which were further evidenced by AFM. Furthermore, PCIPS3 showed excellent scavenging capacities of 2,2-diphenyl-1-picrylhydrazyl radical, superoxide radical, hydroxyl radical, ORAC radical and moderate immunomodulatory activity.
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