Taxonomic group: fungi / Basidiomycota
(Phylum: Basidiomycota)
Organ / tissue: spore
The structure was elucidated in this paperNCBI PubMed ID: 22486826Publication DOI: 10.1016/j.carres.2012.02.029Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: Dong Q <qdong
mail.shcnc.ac.cn>; Ding K <kding
mail.shcnc.ac.cn>
Institutions: Glycobiology and Glycochemistry Lab., Shanghai Institute of Materia Medica, Chinese Academy of Sciences and Infinitus, Shanghai, China, Lee Kum Kee Health Products Group Ltd, Hong Kong, Hong Kong
Ganoderma lucidum is an edible and medicinal mushroom used widely in East Asia. In recent years, its spores have been used as a supplement in combination with other forms of antitumor therapies. The cell wall of Ganoderma lucidum spores contains a high amount of polysaccharides. In this study, a neutral polysaccharide, GLSA50-1B, was isolated from sporoderm-broken spores of Ganoderma lucidum, by hot-water extraction, graded ethanol precipitation, anion-exchange chromatography, and gel permeation chromatography. Using sugar compositional analysis, methylation analysis, partial acid hydrolysis, acetolysis, and NMR and ESI-MS spectroscopy, GLSA50-1B was elucidated to be a novel β-D-glucan featured by a 1,6-linked β-D-Glcp backbone with different length of branches consisting of terminal and 1,4-linked Glcp residues, attached to O-4 of alternative Glc residues in the backbone.
structure, polysaccharide, Ganoderma lucidum, β-(1→6)-glucan
Structure type: structural motif or average structure ; 103000
Location inside paper: p.104, GLSA50-1B, fig.1, table 1
Compound class: O-polysaccharide, glucan
Contained glycoepitopes: IEDB_135614,IEDB_141806,IEDB_142488,IEDB_146664,IEDB_241101,IEDB_983931,SB_192
Methods: 13C NMR, 1H NMR, NMR-2D, methylation, IR, GC-MS, TLC, ESI-MS, acid hydrolysis, GLC, ion-exchange chromatography, extraction, optical rotation measurement, acetolysis, HPGPC, phenol-sulfuric acid assay, evaporation, DEPT, m-hydroxydiphenyl method
Related record ID(s): 47503
NCBI Taxonomy refs (TaxIDs): 5315
Show glycosyltransferases
NMR conditions: in D2O at 298 K
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6
6,4,4 bDGlcp 104.07 74.27 76.78 79.62 75.49 61.34
6,4,4,4 %bDGlcp 104.07 74.27 76.91 70.85 77.16 61.34
6,4 bDGlcp 104.07 74.27 76.78 79.62 75.49 61.34
6 bDGlcp 104.07 74.27 76.78 79.62 76.79 69.39
bDGlcp 104.07 74.27 76.78 70.85 76.11 69.92
1H NMR data:
Linkage Residue H1 H2 H3 H4 H5 H6
6,4,4 bDGlcp 4.62 3.38 3.56 3.82 3.70 3.81-3.98
6,4,4,4 %bDGlcp 4.62 3.38 3.52 3.52 3.49 3.81-3.98
6,4 bDGlcp 4.62 3.38 3.56 3.82 3.70 3.81-3.98
6 bDGlcp 4.58 3.38 3.56 3.82 3.70 3.96-4.28
bDGlcp 4.58 3.38 3.56 3.52 3.68 3.96-4.28
1H/13C HSQC data:
Linkage Residue C1/H1 C2/H2 C3/H3 C4/H4 C5/H5 C6/H6
6,4,4 bDGlcp 104.07/4.62 74.27/3.38 76.78/3.56 79.62/3.82 75.49/3.70 61.34/3.81-3.98
6,4,4,4 %bDGlcp 104.07/4.62 74.27/3.38 76.91/3.52 70.85/3.52 77.16/3.49 61.34/3.81-3.98
6,4 bDGlcp 104.07/4.62 74.27/3.38 76.78/3.56 79.62/3.82 75.49/3.70 61.34/3.81-3.98
6 bDGlcp 104.07/4.58 74.27/3.38 76.78/3.56 79.62/3.82 76.79/3.70 69.39/3.96-4.28
bDGlcp 104.07/4.58 74.27/3.38 76.78/3.56 70.85/3.52 76.11/3.68 69.92/3.96-4.28
1H NMR data:
| Linkage | Residue | H1 | H2 | H3 | H4 | H5 | H6 |
|---|
| 6,4,4 | bDGlcp | 4.62 | 3.38 | 3.56 | 3.82 | 3.70 | 3.81
3.98 |
| 6,4,4,4 | %bDGlcp | 4.62 | 3.38 | 3.52 | 3.52 | 3.49 | 3.81
3.98 |
| 6,4 | bDGlcp | 4.62 | 3.38 | 3.56 | 3.82 | 3.70 | 3.81
3.98 |
| 6 | bDGlcp | 4.58 | 3.38 | 3.56 | 3.82 | 3.70 | 3.96
4.28 |
| | bDGlcp | 4.58 | 3.38 | 3.56 | 3.52 | 3.68 | 3.96
4.28 |
|
13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 |
|---|
| 6,4,4 | bDGlcp | 104.07 | 74.27 | 76.78 | 79.62 | 75.49 | 61.34 |
| 6,4,4,4 | %bDGlcp | 104.07 | 74.27 | 76.91 | 70.85 | 77.16 | 61.34 |
| 6,4 | bDGlcp | 104.07 | 74.27 | 76.78 | 79.62 | 75.49 | 61.34 |
| 6 | bDGlcp | 104.07 | 74.27 | 76.78 | 79.62 | 76.79 | 69.39 |
| | bDGlcp | 104.07 | 74.27 | 76.78 | 70.85 | 76.11 | 69.92 |
|
There is only one chemically distinct structure:
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