CSDB: Search results

Taxonomic group: bacteria / Actinobacteria (Phylum: Actinobacteria)

The structure was elucidated in this paper
Publication DOI: 10.1016/j.foodchem.2010.04.037
Journal NLM ID: 7702639
Publisher: Elsevier Applied Science Publishers
Correspondence: Asker MMS <mohsenmsa

yahoo.com>
Institutions: Department of Microbial Biotechnology, National Research Center, Cairo, Egypt, Department of Microbial Chemistry, National Research Center, Cairo, Egypt

A water-soluble acidic extracellular polysaccharide reaching a maximum concentration of 23.4 g/l growth medium, coded as BSMA, was isolated from the non-pathogenic soil bacteria Brevibacterium otitidis BTS44, by precipitating with two volumes of ethanol. BSMA consisted of arabinose, mannose, glucose and mannouronic acid in ratios of 2.7:3.6:2.1:1.0. No protein was detected in the BSMA fraction, and its molecular weight was about 127 kDa. It has a backbone composed of (1→5)-linked arabinose, (1→6)-linked mannose with three branches attached to O-3 of (1→6)-linked mannose and terminated with either mannose, or mannose and glucose; all the glucose and most of the mannouronic acid are distributed in branches. Partial acid hydrolysis of BSMA gave four sub-fractions termed BSMA-1, BSMA-2, BSMA-3 and BSMA-4. BSMA-1 was composed of arabinose, mannose and trace amounts of mannouronic acid; BSMA-2 was only composed of arabinose and mannose; BSMA-3 was composed of mannose, mannouronic acid and glucose, and BSMA-4 was only composed of mannose and glucose. In the in vitro antioxidant assay, BSMA was found to possess DPPH radical-scavenging activity, with an IC50 value of 120 μg/ml.

structural, polysaccharide, Brevibacterium otitidis BTS 44, radical-scavenging activity

Structure type: structural motif or average structure ; 127000
Location inside paper: Fig.3, BSMA
Compound class: O-polysaccharide, arabinoglucomannan
Contained glycoepitopes: IEDB_130701,IEDB_136907,IEDB_137485,IEDB_140629,IEDB_142488,IEDB_144983,IEDB_144998,IEDB_146664,IEDB_152206,IEDB_164174,IEDB_423115,IEDB_581506,IEDB_983930,IEDB_983931,SB_192,SB_197,SB_44,SB_67,SB_72

Methods: methylation, acid hydrolysis, FTIR, HPLC, GPC, colorimetry, extraction, periodate oxidation, acetylation, reduction, cell growth, radical scavenging assay, ethanol precipitation, evaporation
Biological activity: BSMA possesses strong scavenging effect, which may be comparable to vitamin C
Comments, role: absolute configurations of mannose and glucose residues were assumed by CSDB annotator

NCBI Taxonomy refs (TaxIDs): 53364
Show glycosyltransferases

There are too many chemically distinct structures (~16384), so only one is shown:

-5)?DAraf(1-6)[?DManp(1-3)?DManpA(1-3)?DGlcp(1-3)?DManp(1-3)]?DManp(1-5)?DAraf(1-6)?DManp(1-5)?DAraf(1-6)[?DGlcp(1-4)[?DManp(1-6)]?DGlcp(1-3)?DManpA(1-4)?DManp(1-3)]?DManp(1-5)?DAraf(1-6)?DManp(1-5)?DAraf(1-6)?DManp(1-5)?DAraf(1-6)[?DGlcp(1-4)[?DManp(1-6)]?DGlcp(1-3)?DManpA(1-4)?DManp(1-3)]?DManp(1-5)?DAraf(1-

[*]OC[C@H]1OC(OC[C@H]2OC(OC[C@H]3OC(OC[C@H]4OC(OC[C@H]5OC(OC[C@H]6OC(OC[C@H]7OC(OC[C@H]8OC(OC[C@H]9OC(OC[C@H]%10OC(OC[C@H]%11OC(OC[C@H]%12OC(OC[C@H]%13OC([*])[C@@H](O)[C@@H]%13O)[C@@H](O)[C@@H](OC%13O[C@H](CO)[C@@H](OC%14O[C@H](C(=O)O)[C@@H](O)[C@H](OC%15O[C@H](COC%16O[C@H](CO)[C@@H](O)[C@H](O)[C@@H]%16O)[C@@H](OC%16O[C@H](CO)[C@@H](O)[C@H](O)[C@H]%16O)[C@H](O)[C@H]%15O)[C@@H]%14O)[C@H](O)[C@@H]%13O)[C@@H]%12O)[C@@H](O)[C@@H]%11O)[C@@H](O)[C@@H](O)[C@@H]%10O)[C@@H](O)[C@@H]9O)[C@@H](O)[C@@H](O)[C@@H]8O)[C@@H](O)[C@@H]7O)[C@@H](O)[C@@H](OC7O[C@H](CO)[C@@H](OC8O[C@H](C(=O)O)[C@@H](O)[C@H](OC9O[C@H](COC%10O[C@H](CO)[C@@H](O)[C@H](O)[C@@H]%10O)[C@@H](OC%10O[C@H](CO)[C@@H](O)[C@H](O)[C@H]%10O)[C@H](O)[C@H]9O)[C@@H]8O)[C@H](O)[C@@H]7O)[C@@H]6O)[C@@H](O)[C@@H]5O)[C@@H](O)[C@@H](O)[C@@H]4O)[C@@H](O)[C@@H]3O)[C@@H](O)[C@@H](OC3O[C@H](CO)[C@@H](O)[C@H](OC4O[C@H](CO)[C@@H](O)[C@H](OC5O[C@H](C(=O)O)[C@@H](O)[C@H](OC6O[C@H](CO)[C@@H](O)[C@H](O)[C@@H]6O)[C@@H]5O)[C@H]4O)[C@@H]3O)[C@@H]2O)[C@@H](O)[C@@H]1O

SVG MW SMILES 3D mol Ionize

Taxonomic group: fungi / Basidiomycota (Phylum: Basidiomycota)
Organ / tissue: fruiting body

The structure was elucidated in this paper
NCBI PubMed ID: 25542113
Publication DOI: 10.1016/j.carbpol.2014.10.058
Journal NLM ID: 8307156
Publisher: Elsevier
Correspondence: Liu X <liuxinkui

21cn.com>
Institutions: Department of Medical Records Management, the First Affiliated Hospital of Zhengzhou University, Zhengzhou, China, Department of Radiotherapy, the First Affiliated Hospital of Zhengzhou University, Zhengzhou, China, Nursing Department, Henan Provincial People's Hospital, Zhengzhou, China, Department of Respiratory, the First Affiliated Hospital of Zhengzhou University, Zhengzhou, China, Department of Respiratory, Zhengzhou Central Hospital Affiliated to Zhengzhou University, Zhengzhou, China, Department of Prosthodontics, School of Stomatology, the Fourth Military Medical University, Xi'an, China

A water-soluble polysaccharide, designated as KOMAP, was isolated and purified from the alkaline extract of king oyster mushroom, which was composed of glucose (Glc), mannose (Man) and arabinose (Ara) in a molar ratio of 6.2:2.1:2.0. It had an average molecular weight of 21000 Da. GC-MS analysis revealed that KOMAP was a linear structure of the polymer with a backbone composed of β-1,4-linked glucopyranosyl and β-1,3,6-linked mannopyranosyl units, which was terminated with α-1-linked arabinofuranosyl unit at C-6 position of β-1,3,6-linked mannopyranosyl residue along the main chain in the ratio of 3.1:1. The results in the animal experiment showed that 50, 100 and 200 mg/mL of KOMAP not only inhibited the tumor growth, but also increased relative thymus and spleen indices, LPS- or ConA-induced lymphocytes proliferation, and serum cytokine IL-2, TNF-α, and IFN-γ levels, as well as the activities of NK cells and CTLs in spleen of Renca tumor-bearing mice. In summary, our data indicate that the KOMAP exerts effective immunoregulatory and anti-tumor activities in vivo

polysaccharide, immunomodulatory, antitumor, king oyster mushroom

Structure type: structural motif or average structure ; 21000
Location inside paper: Table 1
Compound class: polysaccharide, arabinoglucomannan
Contained glycoepitopes: IEDB_136907,IEDB_137485,IEDB_142488,IEDB_144983,IEDB_146664,IEDB_152206,IEDB_983930,IEDB_983931,SB_192,SB_44,SB_72

Methods: methylation, IR, GC-MS, ELISA, acid hydrolysis, biological assays, methanolysis, HPLC, extraction, acetylation, methylation analysis, reduction, column chromatography, dialysis, cytokine production, HPGPC, precipitation, phenol-sulfuric acid assay, spectrophotometry, derivatization, evaporation, Sevag method, centrifugation, splenocyte proliferation assay
Biological activity: polysaccharide from the alkaline extract of the king oyster mushroom not only significantly and dose-dependently inhibit the growth of implanted Renca tumor in mice at the doses of 50, 100 and 200 mg/kg, but also improve the immune function by increasing relative spleen and thymus weight, promoting Con A- and LPS-induced splenocyte proliferation, elevating the activities of NK cell and CTL in spleen, and increasing the concentation of IL-2, TNF-α, and IFN-γ in the serum of Renca tumor-bearing mice. Besides, polysaccharide administration does not induce any toxicity to their normal organs, such as liver and kidney

NCBI Taxonomy refs (TaxIDs): 5323
Reference(s) to other database(s): GTC:G73975TX
Show glycosyltransferases

There is only one chemically distinct structure:

[*]O[C@@H]1[C@@H](CO)O[C@@H](O[C@@H]2[C@@H](CO)O[C@@H](O[C@@H]3[C@@H](CO)O[C@@H](O[C@@H]4[C@H](O)[C@H]([*])O[C@H](CO[C@@H]5O[C@@H](CO)[C@H](O)[C@H]5O)[C@H]4O)[C@H](O)[C@H]3O)[C@H](O)[C@H]2O)[C@H](O)[C@H]1O

SVG MW SMILES 3D mol Ionize