Taxonomic group: fungi / Ascomycota
(Phylum: Ascomycota)
Organ / tissue: cell wall
The structure was elucidated in this paperPublication DOI: 10.1081/CAR-120026462Journal NLM ID: 8218151Publisher: Marcel Dekker
Correspondence: Bernabé M <mbernabe
iqog.csic.es>, Leal JA <aleal
cib.csic.es>
Institutions: Centro de Investigaciones Biológicas, Centro de Investigaciones Biológicas (CSIC), Madrid, Spain, Instituto de Química Orgánica, Departamento de Química Orgánica Biológica (CSIC), Madrid, Spain
The alkali-extractable water-soluble polysaccharide F1SS isolated from the cell wall of Cubonia bulbifera has been studied by methylation analysis, NMR spectroscopy, and partial hydrolysis, and its structure was established as structure being n ≈ 4 and m ≈ 1.
polysaccharides, NMR spectroscopy, fungi, Cubonia, Discomycetes, rhamnogalactomannan
Structure type: polymer chemical repeating unit
Location inside paper: Fig.4, table 1, F1SS
Compound class: O-polysaccharide, rhamnogalactomannan
Contained glycoepitopes: IEDB_130701,IEDB_133754,IEDB_136095,IEDB_136105,IEDB_137472,IEDB_144983,IEDB_152206,IEDB_190606,IEDB_225177,IEDB_885823,IEDB_983930,SB_44,SB_67,SB_72
Methods: 13C NMR, 1H NMR, NMR-2D, methylation, GC-MS, acid hydrolysis, GLC, extraction, reduction, column chromatography, cell growth, evaporation
NCBI Taxonomy refs (TaxIDs): 1881958
Show glycosyltransferases
NMR conditions: in D2O at 313 K
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6
6,2,5,2 aLRhap 103.0 71.1 71.3 72.9 70.0 17.5
6,2,5 aLRhap 99.1 79.4 71.3 73.0 70.1 17.6
6,2 bDGalf 106.7 82.2 77.8 83.2 77.0-77.6 61.9
6 aDManp 98.0 75.8 71.1 67.5 72.0 66.4
2,5 aLRhap 99.9 71.2 71.3 ? 70.0 17.6
2 bDGalf 106.7 82.2 77.8 83.2 77.0-77.6 61.9
aDManp 98.0 75.8 71.1 67.5 72.0 66.4
1H NMR data:
Linkage Residue H1 H2 H3 H4 H5 H6
6,2,5,2 aLRhap 4.98 4.07 3.80 3.46 3.74 1.29
6,2,5 aLRhap 5.18 4.03 3.92 3.48 3.91 1.30
6,2 bDGalf 5.11 4.18 4.06 4.17 3.91-3.92 3.76-3.84
6 aDManp 5.03 4.08 3.87 3.81 3.81 3.73-4.02
2,5 aLRhap 5.04 4.02 3.82 ? ? ?
2 bDGalf 5.11 4.18 4.06 4.17 3.91-3.92 3.76-3.84
aDManp 5.03 4.08 3.87 3.81 3.81 3.73-4.02
1H/13C HSQC data:
Linkage Residue C1/H1 C2/H2 C3/H3 C4/H4 C5/H5 C6/H6
6,2,5,2 aLRhap 103.0/4.98 71.1/4.07 71.3/3.80 72.9/3.46 70.0/3.74 17.5/1.29
6,2,5 aLRhap 99.1/5.18 79.4/4.03 71.3/3.92 73.0/3.48 70.1/3.91 17.6/1.30
6,2 bDGalf 106.7/5.11 82.2/4.18 77.8/4.06 83.2/4.17 77.0-77.6/3.91-3.92 61.9/3.76-3.84
6 aDManp 98.0/5.03 75.8/4.08 71.1/3.87 67.5/3.81 72.0/3.81 66.4/3.73-4.02
2,5 aLRhap 99.9/5.04 71.2/4.02 71.3/3.82 ?/? 70.0/? 17.6/?
2 bDGalf 106.7/5.11 82.2/4.18 77.8/4.06 83.2/4.17 77.0-77.6/3.91-3.92 61.9/3.76-3.84
aDManp 98.0/5.03 75.8/4.08 71.1/3.87 67.5/3.81 72.0/3.81 66.4/3.73-4.02
1H NMR data:
| Linkage | Residue | H1 | H2 | H3 | H4 | H5 | H6 |
|---|
| 6,2,5,2 | aLRhap | 4.98 | 4.07 | 3.80 | 3.46 | 3.74 | 1.29 |
| 6,2,5 | aLRhap | 5.18 | 4.03 | 3.92 | 3.48 | 3.91 | 1.30 |
| 6,2 | bDGalf | 5.11 | 4.18 | 4.06 | 4.17 | 3.91
3.92 | 3.76
3.84 |
| 6 | aDManp | 5.03 | 4.08 | 3.87 | 3.81 | 3.81 | 3.73
4.02 |
| 2,5 | aLRhap | 5.04 | 4.02 | 3.82 | ? | ? | ? |
| 2 | bDGalf | 5.11 | 4.18 | 4.06 | 4.17 | 3.91
3.92 | 3.76
3.84 |
| | aDManp | 5.03 | 4.08 | 3.87 | 3.81 | 3.81 | 3.73
4.02 |
|
13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 |
|---|
| 6,2,5,2 | aLRhap | 103.0 | 71.1 | 71.3 | 72.9 | 70.0 | 17.5 |
| 6,2,5 | aLRhap | 99.1 | 79.4 | 71.3 | 73.0 | 70.1 | 17.6 |
| 6,2 | bDGalf | 106.7 | 82.2 | 77.8 | 83.2 | 77.0
77.6 | 61.9 |
| 6 | aDManp | 98.0 | 75.8 | 71.1 | 67.5 | 72.0 | 66.4 |
| 2,5 | aLRhap | 99.9 | 71.2 | 71.3 | ? | 70.0 | 17.6 |
| 2 | bDGalf | 106.7 | 82.2 | 77.8 | 83.2 | 77.0
77.6 | 61.9 |
| | aDManp | 98.0 | 75.8 | 71.1 | 67.5 | 72.0 | 66.4 |
|
The spectrum also has 1 signal at unknown position (not plotted). |
There is only one chemically distinct structure:
report error
Found 1 record.
Displayed record 1
(later renamed to: Pseudombrophila bulbifera CBS 259.65)