Taxonomic group: fungi / Basidiomycota
(Phylum: Basidiomycota)
Organ / tissue: fruiting body
Publication DOI: 10.1248/jhs.55.373Journal NLM ID: 100895795Publisher: Tokyo: Pharmaceutical Society of Japan
Correspondence: Takata T <takajjjj
kanazawa-med.ac.jp>
Institutions: High-Tech Research Center, Kanazawa Medical University, Kahoku, Japan, Department of Pharmacognosy and Chemistry of Natural Products, Graduate School of Natural Science and Technology, Kanazawa University, Kanazawa, Japan, Division of Core Facility, Medical Research Institute, Kanazawa Medical University, Kahoku, Japan, MCProt Biotechnology Company Limited, Kahoku, Japan, Division of Advanced Medicine, Medical Research Institute, Kanazawa Medical University, Kahoku, Japan
Pleurocybella porrigens (P. porrigens) is a traditional food consumed in Japan. Toxicity was first reported in 2004, following which a series of poisonings were reported in 2007. More than 59 people who consumed P. porrigens suffered from similar severe cryptogenic encephalitis, with an overall death rate of approximately 29%. P. porrigens is believed to be a major etiological agent of this disease, but the mechanism of pathogenesis is not clear. To elucidate the toxic properties of P. porrigens in the 2004 and 2007 poisonings, we compared the oligosaccharide constituents of mushroom samples collected in these years with those collected in other years. Water extracts (90°C and 4°C) of P. porrigens were dialyzed, and the oligosaccharides obtained from the high-molecular-weight fraction (>7.8 kDa) were subjected to acid hydrolysis for modification and labeling. Resultant saccharides were analyzed by high performance liquid chromatography on an octadecyl silane (ODS) column. Our analysis revealed that the concentration of N-acetylneuraminic acid (NeuAc) was abundant in all samples, however, N-glycolylneuraminic acid (NeuGc) was present only in significant amounts in the P. porrigens samples collected in 2004 and 2007.
sialic acid, N-acetylneuraminic acid, N-glycolylneuraminic acid, Pleurocybella porrigens, encephalopathy
Structure type: monomer
Location inside paper: Fig.1, NeuAc
Compound class: sialic acid
Contained glycoepitopes: IEDB_136794,IEDB_146100,IEDB_149174,SB_170,SB_171,SB_172,SB_84
Methods: SDS-PAGE, HPLC, extraction, column chromatography, evaporation, HR-FAB-MS, phospho-phenol method
Related record ID(s): 44396
NCBI Taxonomy refs (TaxIDs): 71910Reference(s) to other database(s): GTC:G50850NI
Show glycosyltransferases
There is only one chemically distinct structure:
Taxonomic group: fungi / Basidiomycota
(Phylum: Basidiomycota)
Organ / tissue: fruiting body
Publication DOI: 10.1248/jhs.55.373Journal NLM ID: 100895795Publisher: Tokyo: Pharmaceutical Society of Japan
Correspondence: Takata T <takajjjj
kanazawa-med.ac.jp>
Institutions: High-Tech Research Center, Kanazawa Medical University, Kahoku, Japan, Department of Pharmacognosy and Chemistry of Natural Products, Graduate School of Natural Science and Technology, Kanazawa University, Kanazawa, Japan, Division of Core Facility, Medical Research Institute, Kanazawa Medical University, Kahoku, Japan, MCProt Biotechnology Company Limited, Kahoku, Japan, Division of Advanced Medicine, Medical Research Institute, Kanazawa Medical University, Kahoku, Japan
Pleurocybella porrigens (P. porrigens) is a traditional food consumed in Japan. Toxicity was first reported in 2004, following which a series of poisonings were reported in 2007. More than 59 people who consumed P. porrigens suffered from similar severe cryptogenic encephalitis, with an overall death rate of approximately 29%. P. porrigens is believed to be a major etiological agent of this disease, but the mechanism of pathogenesis is not clear. To elucidate the toxic properties of P. porrigens in the 2004 and 2007 poisonings, we compared the oligosaccharide constituents of mushroom samples collected in these years with those collected in other years. Water extracts (90°C and 4°C) of P. porrigens were dialyzed, and the oligosaccharides obtained from the high-molecular-weight fraction (>7.8 kDa) were subjected to acid hydrolysis for modification and labeling. Resultant saccharides were analyzed by high performance liquid chromatography on an octadecyl silane (ODS) column. Our analysis revealed that the concentration of N-acetylneuraminic acid (NeuAc) was abundant in all samples, however, N-glycolylneuraminic acid (NeuGc) was present only in significant amounts in the P. porrigens samples collected in 2004 and 2007.
sialic acid, N-acetylneuraminic acid, N-glycolylneuraminic acid, Pleurocybella porrigens, encephalopathy
Structure type: monomer
Location inside paper: Fig.1, NeuGc
Compound class: sialic acid
Contained glycoepitopes: IEDB_153758
Methods: SDS-PAGE, HPLC, extraction, column chromatography, evaporation, HR-FAB-MS, phospho-phenol method
Comments, role: the P. porrigens collected in 2004 and 2007 contained large amounts of NeuGc that may be the cause of encephalopathy
Related record ID(s): 44395
NCBI Taxonomy refs (TaxIDs): 71910Reference(s) to other database(s): GTC:G87918MK
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
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