Taxonomic group: fungi / Basidiomycota
(Phylum: Basidiomycota)
Organ / tissue: fruiting body
NCBI PubMed ID: 22829052Publication DOI: 10.1016/j.ijbiomac.2012.07.018Journal NLM ID: 7909578Publisher: Butterworth-Heinemann
Correspondence: tuzc_mail
yahoo.com.cn
Institutions: Albert Einstein College of Medicine, Yeshiva University, New York, NY, USA, State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang, China, College of Life Science, Jiangxi Normal University, Jiangxi, China, College of Life Science and Food Engineering, Nanchang University, Nanchang, China
Dynamic high pressure microfluidization (DHPM) was applied to assist the lentinan extraction. Response surface methodology (RSM), based on Box-Behnken design, was employed to optimize the DHPM-assisted extraction conditions of lentinan. Three main independent variables (DHPM pressure, ratio of water to raw material, extraction temperature) were taken into consideration. A yield of 7.200% was obtained under a modified condition (ratio of water to raw material of 65. mL/g, DHPM pressure of 147. MPa, extraction temperature of 83 °C), which matched well with the predicted value of the model. The molecular weight of the DHPM-assisted extract and hot water extract was 913,329 and 965,361. Da, respectively. Compared to the traditional hot water extraction, the lentinan extracted by DHPM assisting had better scavenging capacity of hydroxyl radical, superoxide anion free radical, 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical and nitrite. It could be concluded that the DHPM was a promising method to enhance the yield and antioxidant activity of lentinan during extraction.
extraction, response surface methodology, lentinan, antioxidation, dynamic high pressure microfluidization
Structure type: polymer chemical repeating unit ; 913329-965361
Location inside paper: abstract
Trivial name: lentinan
Compound class: O-polysaccharide, glucan
Contained glycoepitopes: IEDB_1397514,IEDB_141806,IEDB_142488,IEDB_146664,IEDB_153543,IEDB_158555,IEDB_161166,IEDB_241101,IEDB_558869,IEDB_857743,IEDB_983931,SB_192
Methods: GPC, extraction, phenol-sulfuric acid assay, radical scavenging assay, DHPME
Biological activity: lentinan has the ability of scavenging hydroxyl radical, superoxide radical, DPPH radical and nitrite, and the DHPM-assisted extract consistently exhibited stronger scavenging ability than the hot water extract
Related record ID(s): 42037, 45671, 45824, 48347, 48358, 48474, 48490, 49209, 49976, 49981, 50008, 50010, 50025, 50063, 50097, 50122, 50612, 50618, 50634, 50650, 50651, 50657, 50661, 50666, 50675, 50678, 50679
NCBI Taxonomy refs (TaxIDs): 5353Reference(s) to other database(s): GTC:G14698DR
Show glycosyltransferases
There is only one chemically distinct structure:
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