Taxonomic group: fungi / Ascomycota
(Phylum: Ascomycota)
Organ / tissue: fruiting body
The structure was elucidated in this paperNCBI PubMed ID: 28161087Publication DOI: 10.1016/j.bmcl.2017.01.063Journal NLM ID: 9107377Publisher: Elsevier
Correspondence: yeming123
sina.com
Institutions: Microbial Resources and Application Laboratory, School of Food Science and Engineering, Hefei University of Technology, Hefei, China
A water-soluble exopolysaccharide, designated as LEP-2a, was isolated from Lachnum YM262 and purified by DEAE-Cellulose 52 and Sepharose CL-6B chromatographic columns. LEP-2a was a homogeneous polysaccharide, with a molecular weight of 1.52×105 Da. It was composed of mannose and galactose in a molar ratio of 20.6:1.0. Its structural features were investigated and elucidated by methylation analysis, periodate oxidation and Smith degradation, FT-IR and NMR spectroscopy. Based on obtained data, the backbone of LEP-2a consisted of 1,2-linked-α-d-mannose, 1,3-linked-α-d-mannose, 1,2,6-linked-α-d-mannose and 1,3-linked-β-D-galactose and the side chains were attached to the backbone at O-6 position of 1,2,6-linked-α-d-mannose. In vitro antioxidant activity assay proved that LEP-2a possessed significant scavenging activities on superoxide, hydroxyl and DPPH radical. Furthermore, LEP-2a had strong in vitro moisture-absorption and -retention capacities as compared to chitosan and glycerol. These results suggested that LEP-2a might have a good potential to be applied as a multifunctional cosmetic additive in cosmetics.
Antioxidant, Lachnum exopolysaccharide, moisture absorption, moisture retention, structural features
Structure type: structural motif or average structure ; 152000
Location inside paper: LEP-2a, Fig. 5, table 2
Compound class: mannan
Contained glycoepitopes: IEDB_130701,IEDB_136044,IEDB_136104,IEDB_137472,IEDB_140116,IEDB_141793,IEDB_141794,IEDB_141830,IEDB_143632,IEDB_144983,IEDB_152206,IEDB_153220,IEDB_164174,IEDB_190606,IEDB_241100,IEDB_76933,IEDB_983930,SB_136,SB_165,SB_166,SB_187,SB_195,SB_196,SB_197,SB_198,SB_44,SB_67,SB_7,SB_72,SB_88
Methods: 13C NMR, 1H NMR, GC-MS, periodate oxidation, acetylation, TOCSY, methylation analysis, reduction with NaBH4, HPGPC, COSY, HSQC, FT-IR, TFA hydrolysis, UV-Vis
Biological activity: LEP-2a showed significant scavenging activities on superoxide, hydroxyl and DPPH radical, and strong in vitro moisture-absorption (43% for 72h) and -retention capacities as compared to chitosan and glycerol (47.1%, 28.2% and 16.8% for 72h in silica gel chamber)
Comments, role: LEP-2a polysaccharide structure variant I; published erroneous NMR chemical shifts of #2,2,3,3_aDManp C5 (66.86), #2,2,3_aDManp C5 (66.47), #2,2_bDGalp C5 (66.31), #2,6_aDManp C5 (66.80), #2_aDManp C5 (66.47), #6_aDManp C5 (66.80) and #_aDManp C5 (66.47) were removed by CSDB staff
Related record ID(s): 44579
NCBI Taxonomy refs (TaxIDs): 47817Reference(s) to other database(s): GTC:G18352RM
Show glycosyltransferases
NMR conditions: in D2O
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6
2,2,3,3 aDManp 100.58 78.42 70.39 66.31 ? 61.06
2,2,3 aDManp 102.17 69.64 78.03 73.28 ? 61.08
2,2 bDGalp 99.76 70.24 78.48 73.28 ? 61.06
2,6 aDManp 102.28 70.05 70.39 70.69 ? 61.08
2 aDManp 98.24 78.66 70.39 66.31 ? 65.70
6 aDManp 102.28 70.05 70.39 70.69 ? 61.08
aDManp 98.24 78.66 70.39 66.31 ? 65.70
1H NMR data:
Linkage Residue H1 H2 H3 H4 H5 H6
2,2,3,3 aDManp 5.24 4.06 3.86 3.73 3.62 3.74-3.85
2,2,3 aDManp 5.00 4.16 3.90 3.74 3.70 3.75-3.85
2,2 bDGalp 4.87 3.95 3.84 3.73 3.77 3.74-3.85
2,6 aDManp 5.10 4.03 3.85 3.77 3.66 3.71-3.84
2 aDManp 5.06 4.00 3.86 3.73 3.68 3.97
6 aDManp 5.10 4.03 3.85 3.77 3.66 3.71-3.84
aDManp 5.06 4.00 3.86 3.73 3.68 3.97
1H/13C HSQC data:
Linkage Residue C1/H1 C2/H2 C3/H3 C4/H4 C5/H5 C6/H6
2,2,3,3 aDManp 100.58/5.24 78.42/4.06 70.39/3.86 66.31/3.73 ?/3.62 61.06/3.74-3.85
2,2,3 aDManp 102.17/5.00 69.64/4.16 78.03/3.90 73.28/3.74 ?/3.70 61.08/3.75-3.85
2,2 bDGalp 99.76/4.87 70.24/3.95 78.48/3.84 73.28/3.73 ?/3.77 61.06/3.74-3.85
2,6 aDManp 102.28/5.10 70.05/4.03 70.39/3.85 70.69/3.77 ?/3.66 61.08/3.71-3.84
2 aDManp 98.24/5.06 78.66/4.00 70.39/3.86 66.31/3.73 ?/3.68 65.70/3.97
6 aDManp 102.28/5.10 70.05/4.03 70.39/3.85 70.69/3.77 ?/3.66 61.08/3.71-3.84
aDManp 98.24/5.06 78.66/4.00 70.39/3.86 66.31/3.73 ?/3.68 65.70/3.97
1H NMR data:
| Linkage | Residue | H1 | H2 | H3 | H4 | H5 | H6 |
|---|
| 2,2,3,3 | aDManp | 5.24 | 4.06 | 3.86 | 3.73 | 3.62 | 3.74
3.85 |
| 2,2,3 | aDManp | 5.00 | 4.16 | 3.90 | 3.74 | 3.70 | 3.75
3.85 |
| 2,2 | bDGalp | 4.87 | 3.95 | 3.84 | 3.73 | 3.77 | 3.74
3.85 |
| 2,6 | aDManp | 5.10 | 4.03 | 3.85 | 3.77 | 3.66 | 3.71
3.84 |
| 2 | aDManp | 5.06 | 4.00 | 3.86 | 3.73 | 3.68 | 3.97 |
| 6 | aDManp | 5.10 | 4.03 | 3.85 | 3.77 | 3.66 | 3.71
3.84 |
| | aDManp | 5.06 | 4.00 | 3.86 | 3.73 | 3.68 | 3.97 |
|
13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 |
|---|
| 2,2,3,3 | aDManp | 100.58 | 78.42 | 70.39 | 66.31 | ? | 61.06 |
| 2,2,3 | aDManp | 102.17 | 69.64 | 78.03 | 73.28 | ? | 61.08 |
| 2,2 | bDGalp | 99.76 | 70.24 | 78.48 | 73.28 | ? | 61.06 |
| 2,6 | aDManp | 102.28 | 70.05 | 70.39 | 70.69 | ? | 61.08 |
| 2 | aDManp | 98.24 | 78.66 | 70.39 | 66.31 | ? | 65.70 |
| 6 | aDManp | 102.28 | 70.05 | 70.39 | 70.69 | ? | 61.08 |
| | aDManp | 98.24 | 78.66 | 70.39 | 66.31 | ? | 65.70 |
|
The spectrum also has 7 signals at unknown positions (not plotted). |
There is only one chemically distinct structure:
Taxonomic group: fungi / Ascomycota
(Phylum: Ascomycota)
Organ / tissue: fruiting body
The structure was elucidated in this paperNCBI PubMed ID: 28161087Publication DOI: 10.1016/j.bmcl.2017.01.063Journal NLM ID: 9107377Publisher: Elsevier
Correspondence: yeming123
sina.com
Institutions: Microbial Resources and Application Laboratory, School of Food Science and Engineering, Hefei University of Technology, Hefei, China
A water-soluble exopolysaccharide, designated as LEP-2a, was isolated from Lachnum YM262 and purified by DEAE-Cellulose 52 and Sepharose CL-6B chromatographic columns. LEP-2a was a homogeneous polysaccharide, with a molecular weight of 1.52×105 Da. It was composed of mannose and galactose in a molar ratio of 20.6:1.0. Its structural features were investigated and elucidated by methylation analysis, periodate oxidation and Smith degradation, FT-IR and NMR spectroscopy. Based on obtained data, the backbone of LEP-2a consisted of 1,2-linked-α-d-mannose, 1,3-linked-α-d-mannose, 1,2,6-linked-α-d-mannose and 1,3-linked-β-D-galactose and the side chains were attached to the backbone at O-6 position of 1,2,6-linked-α-d-mannose. In vitro antioxidant activity assay proved that LEP-2a possessed significant scavenging activities on superoxide, hydroxyl and DPPH radical. Furthermore, LEP-2a had strong in vitro moisture-absorption and -retention capacities as compared to chitosan and glycerol. These results suggested that LEP-2a might have a good potential to be applied as a multifunctional cosmetic additive in cosmetics.
Antioxidant, Lachnum exopolysaccharide, moisture absorption, moisture retention, structural features
Structure type: structural motif or average structure ; 152000
Location inside paper: LEP-2a, Fig. 5, table 2
Compound class: mannan
Contained glycoepitopes: IEDB_130701,IEDB_136044,IEDB_136104,IEDB_137472,IEDB_140116,IEDB_141111,IEDB_141793,IEDB_141794,IEDB_141830,IEDB_143632,IEDB_144983,IEDB_152206,IEDB_153220,IEDB_164174,IEDB_164175,IEDB_164176,IEDB_174840,IEDB_190606,IEDB_241100,IEDB_76933,IEDB_983930,SB_136,SB_165,SB_166,SB_187,SB_195,SB_196,SB_197,SB_198,SB_44,SB_67,SB_7,SB_72,SB_88
Methods: 13C NMR, 1H NMR, GC-MS, periodate oxidation, acetylation, TOCSY, methylation analysis, reduction with NaBH4, HPGPC, COSY, HSQC, FT-IR, TFA hydrolysis, UV-Vis
Biological activity: LEP-2a showed significant scavenging activities on superoxide, hydroxyl and DPPH radical, and strong in vitro moisture-absorption (43% for 72h) and -retention capacities as compared to chitosan and glycerol (47.1%, 28.2% and 16.8% for 72h in silica gel chamber)
Comments, role: LEP-2a polysaccharide structure variant I; published erroneous NMR chemical shifts of #2,3,2,3,3_aDManp C5 (66.86), #2,3,2,3_aDManp C5 (66.47), #2,3,2_bDGalp C5 (66.31), #2,3,6_aDManp C5 (66.80), #2,3_aDManp C5 (66.47), #2_aDManp C5 (66.47), #6_aDManp C5 (66.80) and #_aDManp C5 (66.47) were removed by CSDB staff
Related record ID(s): 44578
NCBI Taxonomy refs (TaxIDs): 47817Reference(s) to other database(s): GTC:G81906QR
Show glycosyltransferases
NMR conditions: in D2O
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6
2,3,2,3,3 aDManp 100.58 78.42 70.39 66.31 ? 61.06
2,3,2,3 aDManp 102.17 69.64 78.03 73.28 ? 61.08
2,3,2 bDGalp 99.76 70.24 78.48 73.28 ? 61.06
2,3,6 aDManp 102.28 70.05 70.39 70.69 ? 61.08
2,3 aDManp 98.24 78.66 70.39 66.31 ? 65.70
2 aDManp 102.17 69.64 78.03 73.28 ? 61.08
6 aDManp 102.28 70.05 70.39 70.69 ? 61.08
aDManp 98.24 78.66 70.39 66.31 ? 65.70
1H NMR data:
Linkage Residue H1 H2 H3 H4 H5 H6
2,3,2,3,3 aDManp 5.24 4.06 3.86 3.73 3.62 3.74-3.85
2,3,2,3 aDManp 5.00 4.16 3.90 3.74 3.70 3.75-3.85
2,3,2 bDGalp 4.87 3.95 3.84 3.73 3.77 3.74-3.85
2,3,6 aDManp 5.06 4.00 3.86 3.73 3.68 3.97
2,3 aDManp 5.06 4.00 3.86 3.73 3.68 3.97
2 aDManp 5.00 4.16 3.90 3.74 3.70 3.75-3.85
6 aDManp 5.10 4.03 3.85 3.77 3.66 3.71-3.84
aDManp 5.06 4.00 3.86 3.73 3.68 3.97
1H/13C HSQC data:
Linkage Residue C1/H1 C2/H2 C3/H3 C4/H4 C5/H5 C6/H6
2,3,2,3,3 aDManp 100.58/5.24 78.42/4.06 70.39/3.86 66.31/3.73 ?/3.62 61.06/3.74-3.85
2,3,2,3 aDManp 102.17/5.00 69.64/4.16 78.03/3.90 73.28/3.74 ?/3.70 61.08/3.75-3.85
2,3,2 bDGalp 99.76/4.87 70.24/3.95 78.48/3.84 73.28/3.73 ?/3.77 61.06/3.74-3.85
2,3,6 aDManp 102.28/5.06 70.05/4.00 70.39/3.86 70.69/3.73 ?/3.68 61.08/3.97
2,3 aDManp 98.24/5.06 78.66/4.00 70.39/3.86 66.31/3.73 ?/3.68 65.70/3.97
2 aDManp 102.17/5.00 69.64/4.16 78.03/3.90 73.28/3.74 ?/3.70 61.08/3.75-3.85
6 aDManp 102.28/5.10 70.05/4.03 70.39/3.85 70.69/3.77 ?/3.66 61.08/3.71-3.84
aDManp 98.24/5.06 78.66/4.00 70.39/3.86 66.31/3.73 ?/3.68 65.70/3.97
1H NMR data:
| Linkage | Residue | H1 | H2 | H3 | H4 | H5 | H6 |
|---|
| 2,3,2,3,3 | aDManp | 5.24 | 4.06 | 3.86 | 3.73 | 3.62 | 3.74
3.85 |
| 2,3,2,3 | aDManp | 5.00 | 4.16 | 3.90 | 3.74 | 3.70 | 3.75
3.85 |
| 2,3,2 | bDGalp | 4.87 | 3.95 | 3.84 | 3.73 | 3.77 | 3.74
3.85 |
| 2,3,6 | aDManp | 5.06 | 4.00 | 3.86 | 3.73 | 3.68 | 3.97 |
| 2,3 | aDManp | 5.06 | 4.00 | 3.86 | 3.73 | 3.68 | 3.97 |
| 2 | aDManp | 5.00 | 4.16 | 3.90 | 3.74 | 3.70 | 3.75
3.85 |
| 6 | aDManp | 5.10 | 4.03 | 3.85 | 3.77 | 3.66 | 3.71
3.84 |
| | aDManp | 5.06 | 4.00 | 3.86 | 3.73 | 3.68 | 3.97 |
|
13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 |
|---|
| 2,3,2,3,3 | aDManp | 100.58 | 78.42 | 70.39 | 66.31 | ? | 61.06 |
| 2,3,2,3 | aDManp | 102.17 | 69.64 | 78.03 | 73.28 | ? | 61.08 |
| 2,3,2 | bDGalp | 99.76 | 70.24 | 78.48 | 73.28 | ? | 61.06 |
| 2,3,6 | aDManp | 102.28 | 70.05 | 70.39 | 70.69 | ? | 61.08 |
| 2,3 | aDManp | 98.24 | 78.66 | 70.39 | 66.31 | ? | 65.70 |
| 2 | aDManp | 102.17 | 69.64 | 78.03 | 73.28 | ? | 61.08 |
| 6 | aDManp | 102.28 | 70.05 | 70.39 | 70.69 | ? | 61.08 |
| | aDManp | 98.24 | 78.66 | 70.39 | 66.31 | ? | 65.70 |
|
The spectrum also has 8 signals at unknown positions (not plotted). |
There is only one chemically distinct structure:
report error
Found 2 records.
Displayed records from 1 to 2
(later renamed to: Neodasyscypha YM262)