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Taxonomic group: fungi / Basidiomycota (Phylum: Basidiomycota)
Organ / tissue: fruiting body

The structure was elucidated in this paper
Publication DOI: 10.1080/07328303.2017.1354994
Journal NLM ID: 8218151
Publisher: Marcel Dekker
Correspondence: Hao С <haochangchun

snnu.edu.cn>; Sun R <biophymed

snnu.edu.cn>
Institutions: School of Physics and Information Technology, Shaanxi Normal University, Xi’an, China

A novel water-soluble polysaccharide named PEAP-1 was obtained from Pleurotus eryngii by alkaline extraction and purification by anion-exchange and gel-permeation chromatography. Its structural characteristics were investigated by a combination of chemical and instrumental analysis approaches, including methylation analysis, partial acid hydrolysis, periodate oxidation, Smith degradation, high performance liquid chromatography, gas chromatography, infrared spectroscopy, nuclear magnetic resonance spectroscopy, and gas chromatography-mass spectrometry. The results indicated that the molecular weight of PEAP-1 was about 4.5 × 105 Da and its glucose and galactose molar ratio was 16.9:0.37. It had a backbone of β-(1→3)-linked D-glucan, with a single α-linked D-galactose at the C-6 position for every 45 residues, on an average, along the main chain. Atomic force microscopy and scanning electron microscope were employed to observe the morphology of PEAP-1. It was observed to have long chains with multiple branches.

structure, polysaccharide, morphology, alkaline extraction, Pleurotus eryngii

Structure type: structural motif or average structure ; 450000
Location inside paper: PEAP-1, Fig. 8, Table 4, Fig. 4,5
Compound class: galactan
Contained glycoepitopes: IEDB_136044,IEDB_137472,IEDB_141794,IEDB_142488,IEDB_144998,IEDB_146664,IEDB_156494,IEDB_190606,IEDB_983931,SB_165,SB_166,SB_187,SB_192,SB_195,SB_7,SB_88

Methods: 13C NMR, 1H NMR, IR, GC-MS, GC, Smith degradation, HPLC, periodate oxidation, methylation analysis, SEM, phenol-sulfuric acid method, TFA hydrolysis, PEM
Comments, role: proposed structure of PEAP-1
3D data: structural data

NCBI Taxonomy refs (TaxIDs): 5323
Reference(s) to other database(s): GTC:G64284GV
Show glycosyltransferases

NMR conditions: in D2O at 313 K [as TSV]

13C NMR data:
Linkage	Residue	C1	C2	C3	C4	C5	C6
3	bDGalp	102.9	73.0	84.1	69.4	74.8	60.7
3,3,3,6	aDGlcp	99.7	71.1	76.6	68.8	73.2	60.4
3,3,3	bDGalp	102.9	73.0	84.1	69.4	74.8	60.7
3,3	bDGalp	102.9	73.0	84.1	69.4	74.8	60.7
	bDGalp	102.9	73.0	84.1	69.4	74.8	60.7


1H NMR data:
Linkage	Residue	H1	H2	H3	H4	H5	H6
3	bDGalp	4.45	?	?	?	?	?
3,3,3,6	aDGlcp	5.20	?	?	?	?	?
3,3,3	bDGalp	4.45	?	?	?	?	?
3,3	bDGalp	4.45	?	?	?	?	?
	bDGalp	4.45	?	?	?	?	?


1H/13C HSQC data:
Linkage	Residue	C1/H1	C2/H2	C3/H3	C4/H4	C5/H5	C6/H6
3	bDGalp	102.9/4.45	73.0/?	84.1/?	69.4/?	74.8/?	60.7/?
3,3,3,6	aDGlcp	99.7/5.20	71.1/?	76.6/?	68.8/?	73.2/?	60.4/?
3,3,3	bDGalp	102.9/4.45	73.0/?	84.1/?	69.4/?	74.8/?	60.7/?
3,3	bDGalp	102.9/4.45	73.0/?	84.1/?	69.4/?	74.8/?	60.7/?
	bDGalp	102.9/4.45	73.0/?	84.1/?	69.4/?	74.8/?	60.7/?

¹H NMR data:

Linkage	Residue	H1	H2	H3	H4	H5	H6
3	bDGalp	4.45	?	?	?	?	?
3,3,3,6	aDGlcp	5.20	?	?	?	?	?
3,3,3	bDGalp	4.45	?	?	?	?	?
3,3	bDGalp	4.45	?	?	?	?	?
	bDGalp	4.45	?	?	?	?	?

¹³C NMR data:

Linkage	Residue	C1	C2	C3	C4	C5	C6
3	bDGalp	102.9	73.0	84.1	69.4	74.8	60.7
3,3,3,6	aDGlcp	99.7	71.1	76.6	68.8	73.2	60.4
3,3,3	bDGalp	102.9	73.0	84.1	69.4	74.8	60.7
3,3	bDGalp	102.9	73.0	84.1	69.4	74.8	60.7
	bDGalp	102.9	73.0	84.1	69.4	74.8	60.7

There is only one chemically distinct structure:

[*]O[C@@H]1[C@@H](O)[C@H](O[C@H]2[C@@H](O)[C@@H](CO)O[C@@H](O[C@H]3[C@@H](O)[C@@H](CO)O[C@@H](O[C@H]4[C@@H](O)[C@@H](CO)O[C@@H](O[C@H]5[C@@H](O)[C@@H](CO)O[C@@H](O[C@H]6[C@@H](O)[C@@H](CO)O[C@@H](O[C@H]7[C@@H](O)[C@@H](CO)O[C@@H](O[C@@H]8[C@@H](O)[C@H]([*])O[C@H](CO)[C@@H]8O)[C@@H]7O)[C@@H]6O)[C@@H]5O)[C@@H]4O)[C@@H]3O)[C@@H]2O)O[C@H](CO[C@H]2O[C@H](CO)[C@@H](O)[C@H](O)[C@H]2O)[C@@H]1O

SVG MW SMILES 3D mol Ionize

Warning = sub-repeat ending at bDGalp: large unit count (42) was reduced to 5