Taxonomic group: fungi / Ascomycota
(Phylum: Ascomycota)
The structure was elucidated in this paperNCBI PubMed ID: 23200743Publication DOI: 10.1016/j.fgb.2012.11.003Journal NLM ID: 9607601Publisher: Orlando, FL : Academic Press / Elsevier
Correspondence: jykim
cnu.ac.kr
Institutions: Department of Microbiology and Molecular Biology, College of Bioscience and Biotechnology, Chungnam National University, Daejeon, South Korea, Systems and Synthetic Biology Research Center, Korea Research Institute of Bioscience & Biotechnology, Korea
Sphingolipids are involved in cell differentiation and morphogenesis in eukaryotic cells. In this study, YlLac1p, a ceramide synthase required for glucosylceramide (GlcCer) synthesis, was found to be essential for hyphal growth in Yarrowia lipolytica. Y. lipolytica GlcCer was shown to be composed of a C16:0 fatty acid, which is hydroxylated at C2, and a C18:2 long chain base, which is unsaturated at both C4 and C8 and methylated at C9. Domain swapping analysis revealed that the entire TRAM/Lag1/CLN8 (TLC) domain, not the Lag1 motif, is crucial for the function of YlLac1p. YlDes1p, the C4 desaturase of the ceramide synthesized by YlLac1p, was also required for Y. lipolytica morphogenesis. Both Yllac1D and Yldes1D mutants neither polarize lipid rafts nor form normal vacuoles. Interestingly, mutation in YlPEX5, which encode a peroxisomal targeting signal receptor, partially suppressed the defective hyphal growth of Yllac1D. The Yllac1D Ylpex5D mutant restored the ability to polarize lipid rafts and to form normal vacuoles, although it could not synthesize GlcCer. Taken together, our results suggest that GlcCer or GlcCer derivatives may be involved in hyphal morphogenesis in Y. lipolytica, at least in part, by affecting polarization of lipid rafts and vacuole morphogenesis.
glucosylceramide, Ceramide Synthase, hyphal growth, YlLac1, YlPEX5
Structure type: monomer ; 750
Location inside paper: abstract, GlcCer
Compound class: glycosylceramide
Contained glycoepitopes: IEDB_137339,IEDB_142488,IEDB_146664,IEDB_983931,SB_192,SB_5
Methods: TLC, GC–MS, MALDI–TOF
Biological activity: necessary for hyphal growth
Enzymes that release or process the structure: YlLac1p, YlDes1p, Lcb1/2, Tsc10, Lac1, Des1, Sld1, Mts1, Hsx11
Biosynthesis and genetic data: genetic data, biochemical data
NCBI Taxonomy refs (TaxIDs): 4952
Show glycosyltransferases
There is only one chemically distinct structure:
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