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Wang J, Xu X, Zheng H, Li J, Deng C, Xu Z, Chen J
Structural characterization, chain conformation, and morphology of a β-(1-3)-D-glucan isolated from the fruiting body of Dictyophora indusiata
Journal of Agricultural and Food Chemistry 57(13) (2009)
5918-5924
|
b-D-Glcp-(1-6)-+
|
-3)-b-D-Glcp-(1-3)-b-D-Glcp-(1-3)-b-D-Glcp-(1- |
Show graphically |
Dictyophora indusiata
(NCBI TaxID 146777,
species name lookup)
Taxonomic group: fungi / Basidiomycota
(Phylum: Basidiomycota)
Organ / tissue: fruiting body
The structure was elucidated in this paperNCBI PubMed ID: 19530680Publication DOI: 10.1021/jf9009872Journal NLM ID: 0374755Publisher: American Chemical Society
Correspondence: Chen J <jhchenwhut
126.com>
Institutions: Department of Chemistry, Wuhan University, Wuhan, China, School of Medicine and Pharmaceautics, Jiangnan University, Wuxi, China, College of Chemical Engineering, Wuhan University of Technology, Wuhan, China
A water-soluble glucan, namely, PD3, was isolated from the fruiting body of Dictyophora indusiata as the method reported previously. Its chemical structure was characterized by GC, FTIR, and 13C NMR. The results indicated that PD3 is a (1-3)-β-D-glucan, with (1-6)-β-glucopyranoside side branches. The chain conformation and morphology of PD3 in aqueous solution were investigated by viscometry, rheometer, and laser light scattering (LLS) measurements, atomic force microscopy (AFM), and transmission electron microscopy (TEM), respectively. The weight-average molecular mass (Mw), radius of gyration (Rg), hydrodynamic radius (Rh), and intrinsic viscosity ([η]) of PD3 in water were determined to be 510000, 141 nm, 44 nm, and 1440 cm3/g, respectively, by LLS and viscometry. The structural parameter F (Rg/Rh) of PD3 was calculated to be 3.4, and the [η] dependence of CNaOH of PD3 is similar to that of triple helical polysaccharides Scleroglucan and Lentinan, suggesting that PD3 exists as a triple helical chain in water. This conclusion was further proved by rheological measurement and AFM observation. Interestingly, the [η] of PD3 dramatically decreased in a narrow range concentration of NaOH between 0.18 and 0.22 M, higher than that of Scleroglucan and Lentinan (both less than 0.1 M), indicating the helix-coil conformation transition of PD3 is more difficult than that of Scleroglucan and Lentinan. Moreover, with the increase of concentration, PD3 trends to self-assemble to fibrous aggregates in aqueous solution as measured by TEM.
morphology, structure characterization, Chain conformation, (1-3)-β-D-glucan, Dictyophora indusiata
Structure type: polymer chemical repeating unit ; 510000
Location inside paper: p. 5921, column 1, paragraph 2, table 1, PD3
Compound class: glucan
Contained glycoepitopes: IEDB_1397514,IEDB_141806,IEDB_142488,IEDB_146664,IEDB_153543,IEDB_158555,IEDB_161166,IEDB_241101,IEDB_558869,IEDB_857743,IEDB_983931,SB_192
Methods: 13C NMR, acid hydrolysis, FTIR, gel chromatography, TEM, AFM, laser light scattering, dynamic laser scattering, intrinsic viscosity measurement
Biological activity: the PD3 and RPD3 test groups exhibited obvious anti-tumor activities in vivo on S-180 tumor cells. RPD3 showed higher tumor inhibiting rate suggesting the denature–renature process could significantly enhance the activity of the polysaccharide
3D data: conformation data
Related record ID(s): 41576, 44945, 49106
NCBI Taxonomy refs (TaxIDs): 146777Reference(s) to other database(s): GTC:G66305IS
Show glycosyltransferases
NMR conditions: in D2O at 333(C) K
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6
3,3 bDGlcp 103.8 73.9 86.5 68.7 76.8 61.3
3,6 bDGlcp
3 bDGlcp 103.8 73.9 86.5 69.6 74.8 70.7
bDGlcp 103.8 73.9 86.5 68.7 76.8 61.3
1H NMR data:
missing...
13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 |
|---|
| 3,3 | bDGlcp | 103.8 | 73.9 | 86.5 | 68.7 | 76.8 | 61.3 |
| 3,6 | bDGlcp | |
| 3 | bDGlcp | 103.8 | 73.9 | 86.5 | 69.6 | 74.8 | 70.7 |
| | bDGlcp | 103.8 | 73.9 | 86.5 | 68.7 | 76.8 | 61.3 |
|
There is only one chemically distinct structure:
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Wang J, Xu X, Zheng H, Li J, Deng C, Xu Z, Chen J
Structural characterization, chain conformation, and morphology of a β-(1-3)-D-glucan isolated from the fruiting body of Dictyophora indusiata
Journal of Agricultural and Food Chemistry 57(13) (2009)
5918-5924
|
b-D-Glcp-(1-6)-+ b-D-Glcp-(1-6)-+
| |
-3)-b-D-Glcp-(1-3)-b-D-Glcp-(1-3)-b-D-Glcp-(1-3)-b-D-Glcp-(1-3)-b-D-Glcp-(1- |
Show graphically |
Lentinula edodes
(NCBI TaxID 5353,
species name lookup)
Taxonomic group: fungi / Basidiomycota
(Phylum: Basidiomycota)
Organ / tissue: cell wall
NCBI PubMed ID: 19530680Publication DOI: 10.1021/jf9009872Journal NLM ID: 0374755Publisher: American Chemical Society
Correspondence: Chen J <jhchenwhut
126.com>
Institutions: Department of Chemistry, Wuhan University, Wuhan, China, School of Medicine and Pharmaceautics, Jiangnan University, Wuxi, China, College of Chemical Engineering, Wuhan University of Technology, Wuhan, China
A water-soluble glucan, namely, PD3, was isolated from the fruiting body of Dictyophora indusiata as the method reported previously. Its chemical structure was characterized by GC, FTIR, and 13C NMR. The results indicated that PD3 is a (1-3)-β-D-glucan, with (1-6)-β-glucopyranoside side branches. The chain conformation and morphology of PD3 in aqueous solution were investigated by viscometry, rheometer, and laser light scattering (LLS) measurements, atomic force microscopy (AFM), and transmission electron microscopy (TEM), respectively. The weight-average molecular mass (Mw), radius of gyration (Rg), hydrodynamic radius (Rh), and intrinsic viscosity ([η]) of PD3 in water were determined to be 510000, 141 nm, 44 nm, and 1440 cm3/g, respectively, by LLS and viscometry. The structural parameter F (Rg/Rh) of PD3 was calculated to be 3.4, and the [η] dependence of CNaOH of PD3 is similar to that of triple helical polysaccharides Scleroglucan and Lentinan, suggesting that PD3 exists as a triple helical chain in water. This conclusion was further proved by rheological measurement and AFM observation. Interestingly, the [η] of PD3 dramatically decreased in a narrow range concentration of NaOH between 0.18 and 0.22 M, higher than that of Scleroglucan and Lentinan (both less than 0.1 M), indicating the helix-coil conformation transition of PD3 is more difficult than that of Scleroglucan and Lentinan. Moreover, with the increase of concentration, PD3 trends to self-assemble to fibrous aggregates in aqueous solution as measured by TEM.
morphology, structure characterization, Chain conformation, (1-3)-β-D-glucan, Dictyophora indusiata
Structure type: polymer chemical repeating unit
Location inside paper: p. 5918, column 1, paragraph 1
Trivial name: lentinan, lentinan (SPG), lentinan (LNT), lentinan, β-glucan
Compound class: EPS, O-polysaccharide, cell wall polysaccharide, glucan, polysaccharide, β-glucan, lentinan
Contained glycoepitopes: IEDB_1397514,IEDB_141806,IEDB_142488,IEDB_146664,IEDB_153543,IEDB_158555,IEDB_161166,IEDB_241101,IEDB_558869,IEDB_857743,IEDB_983931,SB_192
Methods: 13C NMR, acid hydrolysis, FTIR, gel chromatography, TEM, AFM, laser light scattering, dynamic laser scattering, intrinsic viscosity measurement
Biological activity: antitumor activity
3D data: conformation data
Related record ID(s): 44944, 48347, 48358, 48474, 48490, 49209
NCBI Taxonomy refs (TaxIDs): 5353Reference(s) to other database(s): GTC:G14698DR, CCSD:
14833, CBank-STR:15884, CA:37339-90-5, GenDB:MT106078
Show glycosyltransferases
There is only one chemically distinct structure:
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Menezes TMF, Campelo MDS, Lima ABN, Neto JFC, Saraiva MM, Costa de Sousa JA, da Costa Gonzaga ML, Leal LKAM, Ribeiro MENP, Ricardo NMPS, Soares SDA
Effects of polysaccharides isolated from mushrooms (Lentinus edodes Berk or Agaricus blazei Murill) on the gelation of Pluronic® F127
Colloids and Surfaces. A, Physicochemical and Engineering Aspects 642 (2022)
128684
Agaricus brasiliensis Murill
(Ancestor NCBI TaxID 307931,
species name lookup)
Taxonomic group: fungi / Basidiomycota
(Phylum: Basidiomycota)
Organ / tissue: mushroom powder
Publication DOI: 10.1016/j.colsurfa.2022.128684Journal NLM ID: 9891752Publisher: Amsterdam, New York: Elsevier
Correspondence: M.E.N.P. Ricardo <elenir.ribeiro
ufc.br>; N.M.P.S. Ricardo <naricard
ufc.br>; S.D.A. Soares <sas
ufc.br>
Institutions: Laboratory of Polymers and Materials Innovation, Department of Organic and Inorganic Chemistry, Federal University of Ceará, Campus of Pici, Zip Code: 60440-900 Fortaleza, CE, Brazil, Center for Pharmaceutical and Cosmetic Studies, Department of Pharmacy, Federal University of Ceará, Porangabussu Campus, Zip Code: 60430-370 Fortaleza, CE, Brazil
This study evaluated the effects of polysaccharides isolated from Lentinus edodes Berk and Agaricus blazei Murill mushrooms on the gelation process of the Pluronic® F127 (F127) copolymer. The structural characterization of these biopolymers suggested the isolation of an β-glucan L. edodes Berk (LEB) and a proteoglycan complex A. blazei Murill (ABM). Both polysaccharides showed good rheological properties for developing pharmaceutical formulations with antioxidant activity and low cytotoxicity in human neutrophils. The characterization of the gels formed from the mixture of polysaccharides with aqueous solutions of F127 showed that the presence of these biopolymers reduces the temperature range in which F127 remains in the gel phase (G’ > G’’). Furthermore, the thermoresponsive behavior of F127 was not lost, which favors the development of hydrogels with potential for topical use.
polysaccharide, Agaricus blazei, Lentinus edodes, copolymer, Pluronic F127
Structure type: fragment of a bigger structure ; 3350000
Location inside paper: p. 128684-5, table 1, ABM
Trivial name: (1,4)-α-glucan, proteoglycan complex, ABM
Contained glycoepitopes: IEDB_140629,IEDB_142488,IEDB_144998,IEDB_146664,IEDB_420417,IEDB_420418,IEDB_420421,IEDB_857742,IEDB_983931,SB_192
Methods: 13C NMR, 1H NMR, FTIR, GPC, extraction, statistical analysis, antioxidant activities, cytotoxicity assay, rheological study, AFM, intrinsic viscosity measurement, F127-polysaccharides gels preparation
Comments, role: NMR temperature was not specified
Related record ID(s): 51790
NCBI Taxonomy refs (TaxIDs): 307931
Show glycosyltransferases
NMR conditions: in D2O
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6
aDGlcp 99.5 71.4 75.1 78.5 70.0 59.0
1H NMR data:
Linkage Residue H1 H2 H3 H4 H5 H6
aDGlcp 5.3 3.4 3.6 3.8 4.0 4.4
1H/13C HSQC data:
Linkage Residue C1/H1 C2/H2 C3/H3 C4/H4 C5/H5 C6/H6
aDGlcp 99.5/5.3 71.4/3.4 75.1/3.6 78.5/3.8 70.0/4.0 59.0/4.4
1H NMR data:
| Linkage | Residue | H1 | H2 | H3 | H4 | H5 | H6 |
|---|
| | aDGlcp | 5.3 | 3.4 | 3.6 | 3.8 | 4.0 | 4.4 |
|
13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 |
|---|
| | aDGlcp | 99.5 | 71.4 | 75.1 | 78.5 | 70.0 | 59.0 |
|
There is only one chemically distinct structure:
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Menezes TMF, Campelo MDS, Lima ABN, Neto JFC, Saraiva MM, Costa de Sousa JA, da Costa Gonzaga ML, Leal LKAM, Ribeiro MENP, Ricardo NMPS, Soares SDA
Effects of polysaccharides isolated from mushrooms (Lentinus edodes Berk or Agaricus blazei Murill) on the gelation of Pluronic® F127
Colloids and Surfaces. A, Physicochemical and Engineering Aspects 642 (2022)
128684
Lentinus edodes Berk
(later renamed to: Lentinula edodes Berk)
(Ancestor NCBI TaxID 5353,
species name lookup)
Agaricus brasiliensis Murill
(Ancestor NCBI TaxID 307931,
species name lookup)
Taxonomic group: fungi / Basidiomycota
(Phylum: Basidiomycota)
Organ / tissue: mushroom powder
Publication DOI: 10.1016/j.colsurfa.2022.128684Journal NLM ID: 9891752Publisher: Amsterdam, New York: Elsevier
Correspondence: M.E.N.P. Ricardo <elenir.ribeiro
ufc.br>; N.M.P.S. Ricardo <naricard
ufc.br>; S.D.A. Soares <sas
ufc.br>
Institutions: Laboratory of Polymers and Materials Innovation, Department of Organic and Inorganic Chemistry, Federal University of Ceará, Campus of Pici, Zip Code: 60440-900 Fortaleza, CE, Brazil, Center for Pharmaceutical and Cosmetic Studies, Department of Pharmacy, Federal University of Ceará, Porangabussu Campus, Zip Code: 60430-370 Fortaleza, CE, Brazil
This study evaluated the effects of polysaccharides isolated from Lentinus edodes Berk and Agaricus blazei Murill mushrooms on the gelation process of the Pluronic® F127 (F127) copolymer. The structural characterization of these biopolymers suggested the isolation of an β-glucan L. edodes Berk (LEB) and a proteoglycan complex A. blazei Murill (ABM). Both polysaccharides showed good rheological properties for developing pharmaceutical formulations with antioxidant activity and low cytotoxicity in human neutrophils. The characterization of the gels formed from the mixture of polysaccharides with aqueous solutions of F127 showed that the presence of these biopolymers reduces the temperature range in which F127 remains in the gel phase (G’ > G’’). Furthermore, the thermoresponsive behavior of F127 was not lost, which favors the development of hydrogels with potential for topical use.
polysaccharide, Agaricus blazei, Lentinus edodes, copolymer, Pluronic F127
Structure type: fragment of a bigger structure ; 954000, 3350000
Location inside paper: p. 128684-5, table 1, LEB, ABM
Trivial name: polysaccharide LEB, proteoglycan complex, ABM
Compound class: β-glucan
Contained glycoepitopes: IEDB_142488,IEDB_144998,IEDB_146664,IEDB_158538,IEDB_983931,SB_192
Methods: 13C NMR, 1H NMR, FTIR, GPC, extraction, statistical analysis, antioxidant activities, cytotoxicity assay, rheological study, AFM, intrinsic viscosity measurement, F127-polysaccharides gels preparation
Comments, role: NMR temperature was not specified
Related record ID(s): 51649
NCBI Taxonomy refs (TaxIDs): 5353,
307931
Show glycosyltransferases
NMR conditions: in D2O
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6
aDGlcp 102.7 72.7 75.3 69.6 74.6 67.9
1H NMR data:
Linkage Residue H1 H2 H3 H4 H5 H6
aDGlcp 4.6 3.3 3.5 3.7 3.9 4.2
1H/13C HSQC data:
Linkage Residue C1/H1 C2/H2 C3/H3 C4/H4 C5/H5 C6/H6
aDGlcp 102.7/4.6 72.7/3.3 75.3/3.5 69.6/3.7 74.6/3.9 67.9/4.2
1H NMR data:
| Linkage | Residue | H1 | H2 | H3 | H4 | H5 | H6 |
|---|
| | aDGlcp | 4.6 | 3.3 | 3.5 | 3.7 | 3.9 | 4.2 |
|
13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 |
|---|
| | aDGlcp | 102.7 | 72.7 | 75.3 | 69.6 | 74.6 | 67.9 |
|
There is only one chemically distinct structure:
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