Taxonomic group: fungi / Basidiomycota
(Phylum: Basidiomycota)
Organ / tissue: fruiting body
The structure was elucidated in this paperNCBI PubMed ID: 23085138Publication DOI: 10.1016/j.ijbiomac.2012.09.028Journal NLM ID: 7909578Publisher: Butterworth-Heinemann
Correspondence: Islam SS <sirajul_1999
yahoo.com>
Institutions: Department of Chemistry and Chemical Technology, Vidyasagar University, Midnapore, India, Department of Biotechnology, Indian Institute of Technology (IIT) Kharagpur, Kharagpur, India, Division of Plant Biology, Bose Institute, Centenary Building, Kolkata, India
A water soluble polysaccharide was isolated from the aqueous extract of the fruit bodies of somatic hybrid mushroom PCH3FB, obtained through protoplast fusion between the strains Pleurotus florida and Calocybe indica var. APK2. The structure of the repeating unit of the polysaccharide was established., on the basis of sugar analysis, methylation analysis, periodate oxidation, and NMR studies (1H, 13C, DEPT-135, DQF-COSY, TOCSY, NOESY, ROESY, HMQC and HMBC). This molecule showed macrophage, splenocyte and thymocyte activations.
NMR spectroscopy, Pleurotus florida, mushroom polysaccharide, Calocybe indica var. APK2, somatic hybrid PCH3FB, immunoactivatio
Structure type: polymer chemical repeating unit
Location inside paper: abstract, table 1, PS
Compound class: polysaccharide
Contained glycoepitopes: IEDB_134624,IEDB_135614,IEDB_136045,IEDB_136906,IEDB_137472,IEDB_141794,IEDB_141806,IEDB_142488,IEDB_142489,IEDB_144562,IEDB_144998,IEDB_146664,IEDB_151528,IEDB_152214,IEDB_174333,IEDB_190606,IEDB_241101,IEDB_983931,SB_163,SB_192,SB_7,SB_86
Methods: 13C NMR, 1H NMR, GLC-MS, acid hydrolysis, GLC, GC, periodate oxidation, optical rotation measurement, ROESY, methylation analysis, dialysis, macrophage activity assay, phenol-sulfuric acid assay, DQF-COSY, HMBC, HMQC, centrifugation, NOESY, DEPT-135, splenocyte proliferation assay, thymocyte proliferation assay, 1H TOSCY
Biological activity: This molecule showed macrophage, splenocyte and thymocyte activations; Maxium SPI is at 25 μg/mL: maximum TPI is at 50 μg/mL
Comments, role: hybrid mushroom PCH3FB was obtained by protoplast fusion of original strains
NCBI Taxonomy refs (TaxIDs): 188765,
282373Reference(s) to other database(s): GTC:G65277IR
Show glycosyltransferases
NMR conditions: in D2O at 300 K
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6
6,6,3,6,6 aDGalp 101.9 68.8 69.7 69.7 69.9 66.8
6,6,3,6 aDGalp 101.9 68.8 69.7 69.7 69.9 66.8
6,6,3,4 aDGlcp 98.2 72.0 73.3 69.9 71.8 61.1
6,6,3 aDGalp 101.8 69.2 69.2 75.9 69.9 67.6
6,6,4 aLFucp 98.2 68.6 69.0 72.1 67.4 16.0
6,6 bDGlcp 103.3 73.4 84.9 76.3 75.3 61.0
6 bDGlcp 103.4 73.4 75.9 69.9 75.9 69.2
bDGlcp 103.4 73.4 75.9 69.9 75.9 69.2
1H NMR data:
Linkage Residue H1 H2 H3 H4 H5 H6
6,6,3,6,6 aDGalp 5.37 3.61 3.82 3.85 4.00 3.69-3.92
6,6,3,6 aDGalp 5.37 3.61 3.82 3.85 4.00 3.69-3.92
6,6,3,4 aDGlcp 5.06 3.83 3.95 3.82 3.89 3.72-3.89
6,6,3 aDGalp 5.06 3.82 3.84 3.63 4.04 3.61-3.95
6,6,4 aLFucp 4.95 3.72 3.85 3.83 4.00 1.22
6,6 bDGlcp 4.51 3.48 3.72 3.61 3.50 3.83-3.91
6 bDGlcp 4.50 3.31 3.47 3.45 3.61 3.85-4.22
bDGlcp 4.50 3.31 3.47 3.45 3.61 3.85-4.22
1H/13C HSQC data:
Linkage Residue C1/H1 C2/H2 C3/H3 C4/H4 C5/H5 C6/H6
6,6,3,6,6 aDGalp 101.9/5.37 68.8/3.61 69.7/3.82 69.7/3.85 69.9/4.00 66.8/3.69-3.92
6,6,3,6 aDGalp 101.9/5.37 68.8/3.61 69.7/3.82 69.7/3.85 69.9/4.00 66.8/3.69-3.92
6,6,3,4 aDGlcp 98.2/5.06 72.0/3.83 73.3/3.95 69.9/3.82 71.8/3.89 61.1/3.72-3.89
6,6,3 aDGalp 101.8/5.06 69.2/3.82 69.2/3.84 75.9/3.63 69.9/4.04 67.6/3.61-3.95
6,6,4 aLFucp 98.2/4.95 68.6/3.72 69.0/3.85 72.1/3.83 67.4/4.00 16.0/1.22
6,6 bDGlcp 103.3/4.51 73.4/3.48 84.9/3.72 76.3/3.61 75.3/3.50 61.0/3.83-3.91
6 bDGlcp 103.4/4.50 73.4/3.31 75.9/3.47 69.9/3.45 75.9/3.61 69.2/3.85-4.22
bDGlcp 103.4/4.50 73.4/3.31 75.9/3.47 69.9/3.45 75.9/3.61 69.2/3.85-4.22
1H NMR data:
| Linkage | Residue | H1 | H2 | H3 | H4 | H5 | H6 |
|---|
| 6,6,3,6,6 | aDGalp | 5.37 | 3.61 | 3.82 | 3.85 | 4.00 | 3.69
3.92 |
| 6,6,3,6 | aDGalp | 5.37 | 3.61 | 3.82 | 3.85 | 4.00 | 3.69
3.92 |
| 6,6,3,4 | aDGlcp | 5.06 | 3.83 | 3.95 | 3.82 | 3.89 | 3.72
3.89 |
| 6,6,3 | aDGalp | 5.06 | 3.82 | 3.84 | 3.63 | 4.04 | 3.61
3.95 |
| 6,6,4 | aLFucp | 4.95 | 3.72 | 3.85 | 3.83 | 4.00 | 1.22 |
| 6,6 | bDGlcp | 4.51 | 3.48 | 3.72 | 3.61 | 3.50 | 3.83
3.91 |
| 6 | bDGlcp | 4.50 | 3.31 | 3.47 | 3.45 | 3.61 | 3.85
4.22 |
| | bDGlcp | 4.50 | 3.31 | 3.47 | 3.45 | 3.61 | 3.85
4.22 |
|
13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 |
|---|
| 6,6,3,6,6 | aDGalp | 101.9 | 68.8 | 69.7 | 69.7 | 69.9 | 66.8 |
| 6,6,3,6 | aDGalp | 101.9 | 68.8 | 69.7 | 69.7 | 69.9 | 66.8 |
| 6,6,3,4 | aDGlcp | 98.2 | 72.0 | 73.3 | 69.9 | 71.8 | 61.1 |
| 6,6,3 | aDGalp | 101.8 | 69.2 | 69.2 | 75.9 | 69.9 | 67.6 |
| 6,6,4 | aLFucp | 98.2 | 68.6 | 69.0 | 72.1 | 67.4 | 16.0 |
| 6,6 | bDGlcp | 103.3 | 73.4 | 84.9 | 76.3 | 75.3 | 61.0 |
| 6 | bDGlcp | 103.4 | 73.4 | 75.9 | 69.9 | 75.9 | 69.2 |
| | bDGlcp | 103.4 | 73.4 | 75.9 | 69.9 | 75.9 | 69.2 |
|
There is only one chemically distinct structure:
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Found 1 record.
Displayed record 1
(later renamed to: Pleurotus sp. Florida x Calocybe indica var. APK2)