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Abdul Salam J, Salam JA, Das N
Enhanced biodegradation of lindane using oil-in-water bio-microemulsion stabilized by biosurfactant produced by a new yeast strain, Pseudozyma VITJzN01
Journal of Microbiology and Biotechnology 23(11) (2013)
1598-1609
Pseudozyma sp. VITJzN01
(NCBI TaxID 1232475,
species name lookup)
Taxonomic group: fungi / Basidiomycota
(Phylum: Basidiomycota)
The structure was elucidated in this paperNCBI PubMed ID: 23928846Publication DOI: 10.4014/jmb.1307.07016Journal NLM ID: 9431852Publisher: Seoul: Korean Society for Microbiology and Biotechnology
Correspondence: Das N <nilanjana00
lycos.com>
Institutions: Bioremediation Lab, School of Biosciences and Technology, VIT University, Tamil Nadu, India, Bioremediation Lab, School of Biosciences and Technology, VIT University, Vellore, Tamil Nadu, India
Organochlorine pesticide residues continue to remain as a major environmental threat worldwide. Lindane is an organochlorine pesticide widely used as an acaricide in medicine and agriculture. In the present study, a new lindane-degrading yeast strain, Pseudozyma VITJzN01, was identified as a copious producer of glycolipid biosurfactant. The glycolipid structure and type were elucidated by FTIR, NMR spectroscopy, and GC-MS analysis. The surface activity and stability of the glycolipid was analyzed. The glycolipids, characterized as mannosylerythritol lipids (MELs), exhibited excellent surface active properties and the surface tension of water was reduced to 29 mN/m. The glycolipid was stable over a wide range of pH, temperature, and salinity, showing a very low CMC of 25 mg/l. Bio-microemulsion of olive oil-in-water (O/W) was prepared using the purified biosurfactant without addition of any synthetic cosurfactants, for lindane solubilization and enhanced degradation assay in liquid and soil slurry. The O/W bio-microemulsions enhanced the solubility of lindane up to 40-folds. Degradation of lindane (700 mg/l) by VITJzN01 in liquid medium amended with bio-microemulsions was found to be enhanced by 36% in 2 days, compared with degradation in 12 days in the absence of bio-microemulsions. Lindane-spiked soil slurry incubated with bio-microemulsions also showed 20-40% enhanced degradation compared with the treatment with glycolipids or yeast alone. This is the first report on lindane degradation by Pseudozyma sp., and application of bio-microemulsions for enhanced lindane degradation. MEL-stabilized bio-microemulsions can serve as a potential tool for enhanced remediation of diverse lindanecontaminated environments
glycolipids, biodegradation, bio-microemulsion, lindane, Pseudozyma sp, Pseudozyma sp.
Structure type: oligomer
Location inside paper: Tables 1-3, fig. 3, MEL-a, MEL-b
Compound class: glycolipid
Contained glycoepitopes: IEDB_114707,IEDB_137485,IEDB_144983,IEDB_152206,IEDB_983930,SB_44,SB_72
Methods: 13C NMR, 1H NMR, IR, GC-MS, TLC, determination of surface tension, FT-IR, GC–MS, anthrone-sulfuric acid method, surface tension measurement
Biosynthesis and genetic data: genetic data
Synthetic data: biosynthesis
Comments, role: MEL-a is acetylated at C4, MEL-b is not; complex mixture of biosurfactants was produced by Pseudozyma sp. VITJzN01 from olive oil; lipid moiety composition: 2.404% (C8:0), 5.485% (C10:0), 2.561% (C12:0), 14.832% (C14:0), 2.733% (C15:0), 47.039% (C16:0), 7.172% (C17:0), 5.328% (C17:1), 4.485 (C19:1); LIP is C8:0, C10:0, C12:0, C14:0, C15:0, C16:0, C17:0, C17:1, C19:1
NCBI Taxonomy refs (TaxIDs): 1232475
Show glycosyltransferases
NMR conditions: in CDCl3 at 303 K
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6
1,2 LIP
1,3 LIP
1,4 %Ac 171.5-171.8 20.6
1,6 Ac 171.5-171.8 20.6
1 bDManp 99.2 70.5 71.6 65.3 74.9 63.5
xDEry-ol 72.5 72.4 71.8 64.7
1H NMR data:
Linkage Residue H1 H2 H3 H4 H5 H6
1,2 LIP
1,3 LIP
1,4 %Ac - 2.04-2.18
1,6 Ac - 2.04-2.18
1 bDManp 4.78 5.51 5.08 5.26 3.72 4.23
xDEry-ol 3.98-4.02 3.72 3.72 3.72
1H/13C HSQC data:
Linkage Residue C1/H1 C2/H2 C3/H3 C4/H4 C5/H5 C6/H6
1,2 LIP
1,3 LIP
1,4 %Ac 20.6/2.04-2.18
1,6 Ac 20.6/2.04-2.18
1 bDManp 99.2/4.78 70.5/5.51 71.6/5.08 65.3/5.26 74.9/3.72 63.5/4.23
xDEry-ol 72.5/3.98-4.02 72.4/3.72 71.8/3.72 64.7/3.72
1H NMR data:
| Linkage | Residue | H1 | H2 | H3 | H4 | H5 | H6 |
|---|
| 1,2 | LIP | |
| 1,3 | LIP | |
| 1,4 | %Ac |
| 2.04
2.18 | |
| 1,6 | Ac |
| 2.04
2.18 | |
| 1 | bDManp | 4.78 | 5.51 | 5.08 | 5.26 | 3.72 | 4.23 |
| | xDEry-ol | 3.98
4.02 | 3.72 | 3.72 | 3.72 | |
|
13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 |
|---|
| 1,2 | LIP | |
| 1,3 | LIP | |
| 1,4 | %Ac | 171.5
171.8 | 20.6 | |
| 1,6 | Ac | 171.5
171.8 | 20.6 | |
| 1 | bDManp | 99.2 | 70.5 | 71.6 | 65.3 | 74.9 | 63.5 |
| | xDEry-ol | 72.5 | 72.4 | 71.8 | 64.7 | |
|
There is only one chemically distinct structure:
Expand this record
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Abdul Salam J, Salam JA, Das N
Enhanced biodegradation of lindane using oil-in-water bio-microemulsion stabilized by biosurfactant produced by a new yeast strain, Pseudozyma VITJzN01
Journal of Microbiology and Biotechnology 23(11) (2013)
1598-1609
Pseudozyma sp. VITJzN01
(NCBI TaxID 1232475,
species name lookup)
Taxonomic group: fungi / Basidiomycota
(Phylum: Basidiomycota)
The structure was elucidated in this paperNCBI PubMed ID: 23928846Publication DOI: 10.4014/jmb.1307.07016Journal NLM ID: 9431852Publisher: Seoul: Korean Society for Microbiology and Biotechnology
Correspondence: Das N <nilanjana00
lycos.com>
Institutions: Bioremediation Lab, School of Biosciences and Technology, VIT University, Tamil Nadu, India, Bioremediation Lab, School of Biosciences and Technology, VIT University, Vellore, Tamil Nadu, India
Organochlorine pesticide residues continue to remain as a major environmental threat worldwide. Lindane is an organochlorine pesticide widely used as an acaricide in medicine and agriculture. In the present study, a new lindane-degrading yeast strain, Pseudozyma VITJzN01, was identified as a copious producer of glycolipid biosurfactant. The glycolipid structure and type were elucidated by FTIR, NMR spectroscopy, and GC-MS analysis. The surface activity and stability of the glycolipid was analyzed. The glycolipids, characterized as mannosylerythritol lipids (MELs), exhibited excellent surface active properties and the surface tension of water was reduced to 29 mN/m. The glycolipid was stable over a wide range of pH, temperature, and salinity, showing a very low CMC of 25 mg/l. Bio-microemulsion of olive oil-in-water (O/W) was prepared using the purified biosurfactant without addition of any synthetic cosurfactants, for lindane solubilization and enhanced degradation assay in liquid and soil slurry. The O/W bio-microemulsions enhanced the solubility of lindane up to 40-folds. Degradation of lindane (700 mg/l) by VITJzN01 in liquid medium amended with bio-microemulsions was found to be enhanced by 36% in 2 days, compared with degradation in 12 days in the absence of bio-microemulsions. Lindane-spiked soil slurry incubated with bio-microemulsions also showed 20-40% enhanced degradation compared with the treatment with glycolipids or yeast alone. This is the first report on lindane degradation by Pseudozyma sp., and application of bio-microemulsions for enhanced lindane degradation. MEL-stabilized bio-microemulsions can serve as a potential tool for enhanced remediation of diverse lindanecontaminated environments
glycolipids, biodegradation, bio-microemulsion, lindane, Pseudozyma sp, Pseudozyma sp.
Structure type: monomer
Location inside paper: MEL-a, Table 1, Table 2, fig. 3
Trivial name: mannosylerythritol lipids (MELs)
Compound class: glycolipid, mannosylerythritol
Contained glycoepitopes: IEDB_114707,IEDB_137485,IEDB_144983,IEDB_152206,IEDB_983930,SB_44,SB_72
Methods: 13C NMR, 1H NMR, IR, GC-MS, TLC, determination of surface tension, FT-IR, GC–MS, anthrone-sulfuric acid method, surface tension measurement
Comments, role: The major fatty acids in this mannosylerythritol is hexadecanoic acid (47%) and tetradecanoeic acid (14%). LIP is pre-tail hydroxy non- or monounsaturated fatty acid, number of carbon atoms 8-16
Related record ID(s): 45292
NCBI Taxonomy refs (TaxIDs): 1232475
Show glycosyltransferases
NMR conditions: in CDCl3
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6
4,2 LIP
4,3 LIP
4,4 Ac 171.5 20.6
4,6 Ac ? ?
4 bDManp 99.2 70.5 71.6 65.3 74.9 63.5
xDEry-ol 64.7 71.8 72.4 72.5
1H NMR data:
Linkage Residue H1 H2 H3 H4 H5 H6
4,2 LIP
4,3 LIP
4,4 Ac - 2.04
4,6 Ac - 2.12
4 bDManp 4.78 5.51 5.08 5.26 3.72 4.23
xDEry-ol 3.72 3.72 3.72 3.98-4.02
1H/13C HSQC data:
Linkage Residue C1/H1 C2/H2 C3/H3 C4/H4 C5/H5 C6/H6
4,2 LIP
4,3 LIP
4,4 Ac 20.6/2.04
4,6 Ac ?/2.12
4 bDManp 99.2/4.78 70.5/5.51 71.6/5.08 65.3/5.26 74.9/3.72 63.5/4.23
xDEry-ol 64.7/3.72 71.8/3.72 72.4/3.72 72.5/3.98-4.02
1H NMR data:
| Linkage | Residue | H1 | H2 | H3 | H4 | H5 | H6 |
|---|
| 4,2 | LIP | |
| 4,3 | LIP | |
| 4,4 | Ac |
| 2.04 | |
| 4,6 | Ac |
| 2.12 | |
| 4 | bDManp | 4.78 | 5.51 | 5.08 | 5.26 | 3.72 | 4.23 |
| | xDEry-ol | 3.72 | 3.72 | 3.72 | 3.98
4.02 | |
|
13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 |
|---|
| 4,2 | LIP | |
| 4,3 | LIP | |
| 4,4 | Ac | 171.5 | 20.6 | |
| 4,6 | Ac | ? | ? | |
| 4 | bDManp | 99.2 | 70.5 | 71.6 | 65.3 | 74.9 | 63.5 |
| | xDEry-ol | 64.7 | 71.8 | 72.4 | 72.5 | |
|
The spectrum also has 2 signals at unknown positions (not plotted). |
There is only one chemically distinct structure:
Expand this record
Collapse this record
Abdul Salam J, Salam JA, Das N
Enhanced biodegradation of lindane using oil-in-water bio-microemulsion stabilized by biosurfactant produced by a new yeast strain, Pseudozyma VITJzN01
Journal of Microbiology and Biotechnology 23(11) (2013)
1598-1609
Pseudozyma sp. VITJzN01
(NCBI TaxID 1232475,
species name lookup)
Taxonomic group: fungi / Basidiomycota
(Phylum: Basidiomycota)
The structure was elucidated in this paperNCBI PubMed ID: 23928846Publication DOI: 10.4014/jmb.1307.07016Journal NLM ID: 9431852Publisher: Seoul: Korean Society for Microbiology and Biotechnology
Correspondence: Das N <nilanjana00
lycos.com>
Institutions: Bioremediation Lab, School of Biosciences and Technology, VIT University, Tamil Nadu, India, Bioremediation Lab, School of Biosciences and Technology, VIT University, Vellore, Tamil Nadu, India
Organochlorine pesticide residues continue to remain as a major environmental threat worldwide. Lindane is an organochlorine pesticide widely used as an acaricide in medicine and agriculture. In the present study, a new lindane-degrading yeast strain, Pseudozyma VITJzN01, was identified as a copious producer of glycolipid biosurfactant. The glycolipid structure and type were elucidated by FTIR, NMR spectroscopy, and GC-MS analysis. The surface activity and stability of the glycolipid was analyzed. The glycolipids, characterized as mannosylerythritol lipids (MELs), exhibited excellent surface active properties and the surface tension of water was reduced to 29 mN/m. The glycolipid was stable over a wide range of pH, temperature, and salinity, showing a very low CMC of 25 mg/l. Bio-microemulsion of olive oil-in-water (O/W) was prepared using the purified biosurfactant without addition of any synthetic cosurfactants, for lindane solubilization and enhanced degradation assay in liquid and soil slurry. The O/W bio-microemulsions enhanced the solubility of lindane up to 40-folds. Degradation of lindane (700 mg/l) by VITJzN01 in liquid medium amended with bio-microemulsions was found to be enhanced by 36% in 2 days, compared with degradation in 12 days in the absence of bio-microemulsions. Lindane-spiked soil slurry incubated with bio-microemulsions also showed 20-40% enhanced degradation compared with the treatment with glycolipids or yeast alone. This is the first report on lindane degradation by Pseudozyma sp., and application of bio-microemulsions for enhanced lindane degradation. MEL-stabilized bio-microemulsions can serve as a potential tool for enhanced remediation of diverse lindanecontaminated environments
glycolipids, biodegradation, bio-microemulsion, lindane, Pseudozyma sp, Pseudozyma sp.
Structure type: monomer
Location inside paper: MEL-b, Table 1, Table 2, fig. 3
Trivial name: mannosylerythritol lipids (MELs)
Compound class: glycolipid, mannosylerythritol
Contained glycoepitopes: IEDB_114707,IEDB_137485,IEDB_144983,IEDB_152206,IEDB_983930,SB_44,SB_72
Methods: 13C NMR, 1H NMR, IR, GC-MS, TLC, determination of surface tension, FT-IR, GC–MS, anthrone-sulfuric acid method, surface tension measurement
Comments, role: The major fatty acids in this mannosylerythritol is hexadecanoic acid (47%) and tetradecanoeic acid (14%). LIP is pre-tail hydroxy non- or monounsaturated fatty acid, number of carbon atoms 8-16
Related record ID(s): 45291
NCBI Taxonomy refs (TaxIDs): 1232475
Show glycosyltransferases
NMR conditions: in CDCl3
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6
4,2 LIP
4,3 LIP
4,6 Ac ? 20.6
4 bDManp 99.2 70.5 71.6 65.3 74.9 63.5
xDEry-ol 64.7 71.8 72.4 72.5
1H NMR data:
Linkage Residue H1 H2 H3 H4 H5 H6
4,2 LIP
4,3 LIP
4,6 Ac - 2.12
4 bDManp 4.78 5.51 5.08 5.26 3.72 4.23
xDEry-ol 3.72 3.72 3.72 4.02
1H/13C HSQC data:
Linkage Residue C1/H1 C2/H2 C3/H3 C4/H4 C5/H5 C6/H6
4,2 LIP
4,3 LIP
4,6 Ac 20.6/2.12
4 bDManp 99.2/4.78 70.5/5.51 71.6/5.08 65.3/5.26 74.9/3.72 63.5/4.23
xDEry-ol 64.7/3.72 71.8/3.72 72.4/3.72 72.5/4.02
1H NMR data:
| Linkage | Residue | H1 | H2 | H3 | H4 | H5 | H6 |
|---|
| 4,2 | LIP | |
| 4,3 | LIP | |
| 4,6 | Ac |
| 2.12 | |
| 4 | bDManp | 4.78 | 5.51 | 5.08 | 5.26 | 3.72 | 4.23 |
| | xDEry-ol | 3.72 | 3.72 | 3.72 | 4.02 | |
|
13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 |
|---|
| 4,2 | LIP | |
| 4,3 | LIP | |
| 4,6 | Ac | ? | 20.6 | |
| 4 | bDManp | 99.2 | 70.5 | 71.6 | 65.3 | 74.9 | 63.5 |
| | xDEry-ol | 64.7 | 71.8 | 72.4 | 72.5 | |
|
The spectrum also has 1 signal at unknown position (not plotted). |
There is only one chemically distinct structure:
Expand this record
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