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Rodríguez JE, Ramírez AS, Salas LP, Helguera-Repetto C, Gonzalez-y-Merchand J, Soto CY, Hernández-Pando R
Transcription of genes involved in sulfolipid and polyacyltrehalose biosynthesis of Mycobacterium tuberculosis in experimental latent tuberculosis infection
PLoS One 8(3) (2013)
e58378
|
Subst1-(1-6)-+
|
Subst1-(1-6)-+ |
| |
Subst2-(1-3)-a-D-Glcp-(1-1)-a-D-Glcp
| |
Pam-(1-2)-+ S-2)-+
Subst1 = SMILES CCCCCCCCCCCCCCCC(O)C(C)CC(C)CC(C)CC(C)CC(C)CC(C)CC(C)CC(C)C(=O){1}O;
Subst2 = SMILES CCCCCCCCCCCCCCCCC(C)CC(C)CC(C)CC(C)CC(C)CC(C)CC(C)C(=O){1}O |
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Mycobacterium tuberculosis H37Rv
(NCBI TaxID 83332,
species name lookup)
Taxonomic group: bacteria / Actinobacteria
(Phylum: Actinobacteria)
Associated disease: tuberculosis [ICD11:
XN1N2 
];
infection due to Mycobacterium tuberculosis [ICD11:
XN1N2 ]
NCBI PubMed ID: 23472191Publication DOI: 10.1371/journal.pone.0058378Journal NLM ID: 101285081Publisher: San Francisco, CA: Public Library of Science
Correspondence: rhdezpando
hotmail.com
Institutions: Departamento de Quımica, Facultad de Ciencias, Universidad Nacional de Colombia, Bogota, Colombia, Laboratorio de Microbiologıa Molecular, Escuela Nacional de Ciencias Biologicas, Instituto Politecnico Nacional, Mexico DF, Mexico, Seccion de Patologıa Experimental, Instituto Nacional de Ciencias Medicas y Nutricion Salvador Zubiran, Mexico DF, Mexico
The Influence of trehalose-based glycolipids in the virulence of Mycobacterium tuberculosis (Mtb) is recognised; however, the actual role of these cell-wall glycolipids in latent infection is unknown. As an initial approach, we determined by two-dimensional thin-layer chromatography the sulfolipid (SL) and diacyltrehalose/polyacyltrehalose (DAT/PAT) profile of the cell wall of hypoxic Mtb. Then, qRT-PCR was extensively conducted to determine the transcription profile of genes involved in the biosynthesis of these glycolipids in non-replicating persistent 1 (NRP1) and anaerobiosis (NRP2) models of hypoxia (Wayne model), and murine models of chronic and progressive pulmonary tuberculosis. A diminished content of SL and increased amounts of glycolipids with chromatographic profile similar to DAT were detected in Mtb grown in the NRP2 stage. A striking decrease in the transcription of mmpL8 and mmpL10 transporter genes and increased transcription of the pks (polyketidesynthase) genes involved in SL and DAT biosynthesis were detected in both the NRP2 stage and the murine model of chronic infection. All genes were found to be up-regulated in the progressive disease. These results suggest that SL production is diminished during latent infection and the DAT/PAT precursors can be accumulated inside tubercle bacilli and are possibly used in reactivation processes
virulence, biogenesis, acyltransferases, nonreplicating persistence, cell-wall, differential expression, pulmonary tuberculosis, hypoxic conditions, neutral-red, sulfoglycolipids
Structure type: oligomer
Location inside paper: fig.1, SL-I
Contained glycoepitopes: IEDB_141181,IEDB_142488,IEDB_144998,IEDB_146664,IEDB_742521,IEDB_983931,SB_192
Methods: TLC, Neutral-red test, qRT-PCR
Enzymes that release or process the structure: PapA1, PapA2, Pks2
Related record ID(s): 45301, 45302
NCBI Taxonomy refs (TaxIDs): 83332
Show glycosyltransferases
There is only one chemically distinct structure:
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Rodríguez JE, Ramírez AS, Salas LP, Helguera-Repetto C, Gonzalez-y-Merchand J, Soto CY, Hernández-Pando R
Transcription of genes involved in sulfolipid and polyacyltrehalose biosynthesis of Mycobacterium tuberculosis in experimental latent tuberculosis infection
PLoS One 8(3) (2013)
e58378
|
Subst-(1-3)-+
|
17HOSte-(1-2)-+ |
| |
Subst-(1-4)-+ | |
| | |
Subst-(1-2)-a-D-Glcp-(1-1)-a-D-Glcp
|
17HOSte-(1-6)-+
Subst = SMILES CCCCCCCCCCCCCCCCCCC(C)CC(C)/C=C(C)/C(=O){1}O |
Show graphically |
Mycobacterium tuberculosis H37Rv
(NCBI TaxID 83332,
species name lookup)
Taxonomic group: bacteria / Actinobacteria
(Phylum: Actinobacteria)
Associated disease: tuberculosis [ICD11:
XN1N2 ];
infection due to Mycobacterium tuberculosis [ICD11:
XN1N2 ]
NCBI PubMed ID: 23472191Publication DOI: 10.1371/journal.pone.0058378Journal NLM ID: 101285081Publisher: San Francisco, CA: Public Library of Science
Correspondence: rhdezpando
hotmail.com
Institutions: Departamento de Quımica, Facultad de Ciencias, Universidad Nacional de Colombia, Bogota, Colombia, Laboratorio de Microbiologıa Molecular, Escuela Nacional de Ciencias Biologicas, Instituto Politecnico Nacional, Mexico DF, Mexico, Seccion de Patologıa Experimental, Instituto Nacional de Ciencias Medicas y Nutricion Salvador Zubiran, Mexico DF, Mexico
The Influence of trehalose-based glycolipids in the virulence of Mycobacterium tuberculosis (Mtb) is recognised; however, the actual role of these cell-wall glycolipids in latent infection is unknown. As an initial approach, we determined by two-dimensional thin-layer chromatography the sulfolipid (SL) and diacyltrehalose/polyacyltrehalose (DAT/PAT) profile of the cell wall of hypoxic Mtb. Then, qRT-PCR was extensively conducted to determine the transcription profile of genes involved in the biosynthesis of these glycolipids in non-replicating persistent 1 (NRP1) and anaerobiosis (NRP2) models of hypoxia (Wayne model), and murine models of chronic and progressive pulmonary tuberculosis. A diminished content of SL and increased amounts of glycolipids with chromatographic profile similar to DAT were detected in Mtb grown in the NRP2 stage. A striking decrease in the transcription of mmpL8 and mmpL10 transporter genes and increased transcription of the pks (polyketidesynthase) genes involved in SL and DAT biosynthesis were detected in both the NRP2 stage and the murine model of chronic infection. All genes were found to be up-regulated in the progressive disease. These results suggest that SL production is diminished during latent infection and the DAT/PAT precursors can be accumulated inside tubercle bacilli and are possibly used in reactivation processes
virulence, biogenesis, acyltransferases, nonreplicating persistence, cell-wall, differential expression, pulmonary tuberculosis, hypoxic conditions, neutral-red, sulfoglycolipids
Structure type: oligomer
Location inside paper: fig.1, PAT
Contained glycoepitopes: IEDB_142488,IEDB_144998,IEDB_146664,IEDB_742521,IEDB_983931,SB_192
Methods: TLC, Neutral-red test, qRT-PCR
Enzymes that release or process the structure: PapA3, Pks3/4
Related record ID(s): 45300, 45302
NCBI Taxonomy refs (TaxIDs): 83332
Show glycosyltransferases
There is only one chemically distinct structure:
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Rodríguez JE, Ramírez AS, Salas LP, Helguera-Repetto C, Gonzalez-y-Merchand J, Soto CY, Hernández-Pando R
Transcription of genes involved in sulfolipid and polyacyltrehalose biosynthesis of Mycobacterium tuberculosis in experimental latent tuberculosis infection
PLoS One 8(3) (2013)
e58378
|
Subst1-(1-3)-+
|
17HOSte-(1-2)-a-D-Glcp-(1-1)-a-D-Glcp
Subst1 = SMILES CCCCCCCCCCCCCCCCCCC(C)CC(C)C(=O){1}O |
Show graphically |
Mycobacterium tuberculosis H37Rv
(NCBI TaxID 83332,
species name lookup)
Taxonomic group: bacteria / Actinobacteria
(Phylum: Actinobacteria)
Associated disease: tuberculosis [ICD11:
XN1N2 ];
infection due to Mycobacterium tuberculosis [ICD11:
XN1N2 ]
NCBI PubMed ID: 23472191Publication DOI: 10.1371/journal.pone.0058378Journal NLM ID: 101285081Publisher: San Francisco, CA: Public Library of Science
Correspondence: rhdezpando
hotmail.com
Institutions: Departamento de Quımica, Facultad de Ciencias, Universidad Nacional de Colombia, Bogota, Colombia, Laboratorio de Microbiologıa Molecular, Escuela Nacional de Ciencias Biologicas, Instituto Politecnico Nacional, Mexico DF, Mexico, Seccion de Patologıa Experimental, Instituto Nacional de Ciencias Medicas y Nutricion Salvador Zubiran, Mexico DF, Mexico
The Influence of trehalose-based glycolipids in the virulence of Mycobacterium tuberculosis (Mtb) is recognised; however, the actual role of these cell-wall glycolipids in latent infection is unknown. As an initial approach, we determined by two-dimensional thin-layer chromatography the sulfolipid (SL) and diacyltrehalose/polyacyltrehalose (DAT/PAT) profile of the cell wall of hypoxic Mtb. Then, qRT-PCR was extensively conducted to determine the transcription profile of genes involved in the biosynthesis of these glycolipids in non-replicating persistent 1 (NRP1) and anaerobiosis (NRP2) models of hypoxia (Wayne model), and murine models of chronic and progressive pulmonary tuberculosis. A diminished content of SL and increased amounts of glycolipids with chromatographic profile similar to DAT were detected in Mtb grown in the NRP2 stage. A striking decrease in the transcription of mmpL8 and mmpL10 transporter genes and increased transcription of the pks (polyketidesynthase) genes involved in SL and DAT biosynthesis were detected in both the NRP2 stage and the murine model of chronic infection. All genes were found to be up-regulated in the progressive disease. These results suggest that SL production is diminished during latent infection and the DAT/PAT precursors can be accumulated inside tubercle bacilli and are possibly used in reactivation processes
virulence, biogenesis, acyltransferases, nonreplicating persistence, cell-wall, differential expression, pulmonary tuberculosis, hypoxic conditions, neutral-red, sulfoglycolipids
Structure type: oligomer
Location inside paper: fig.1, DAT
Contained glycoepitopes: IEDB_142488,IEDB_144998,IEDB_146664,IEDB_742521,IEDB_983931,SB_192
Methods: TLC, Neutral-red test, qRT-PCR
Enzymes that release or process the structure: Pks3/4
Related record ID(s): 45300, 45301
NCBI Taxonomy refs (TaxIDs): 83332
Show glycosyltransferases
There is only one chemically distinct structure:
Expand this record
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