Found 4 records.
Displayed records from 1 to 4
Expand all records
Collapse all records
Show all as text (SweetDB notation)
Show all graphically (SNFG notation)
Fan LL, Dong YC, Fan YF, Zhang J, Chen QH
Production and identification of mannosylerythritol lipid-A homologs from the ustilaginomycetous yeast Pseudozyma aphidis ZJUDM34
Carbohydrate Research 392 (2014)
1-6
Pseudozyma aphidis ZJUDM34
(Ancestor NCBI TaxID 84754,
species name lookup)
Ustilago maydis
(NCBI TaxID 5270,
species name lookup)
Taxonomic group: fungi / Basidiomycota
(Phylum: Basidiomycota)
The structure was elucidated in this paperNCBI PubMed ID: 24814655Publication DOI: 10.1016/j.carres.2014.04.013Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: Chen QH <chenqh
zju.edu.cn>
Institutions: School of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou, China, Fuli Institute of Food Science, Zhejiang University, Hangzhou, China
Mannosylerythritol lipids (MELs) are mainly produced by strains of the genus Pseudozyma and by Ustilago maydis. These glycolipid biosurfactants exhibit not only excellent surface-active properties but also versatile bioactivities. Mannosylerythritol lipid-A (MEL-A) is worth investigating due to its self-assembling property. In this work, crude MELs were produced by resting Pseudozyma aphidis ZJUDM34 cells using different culture media. MEL-A fractions were isolated and identified using high-performance liquid chromatography combined with mass spectrometry (HPLC-MS) and gas chromatography combined with mass spectrometry (GC-MS). The results showed that MEL-A homologs had long unsaturated fatty acid chains, and the chain lengths range from C8 to C20. Nuclear magnetic resonance (NMR) was employed to confirm the chemical structures of the MEL-A homologs. Fermentation medium without NaNO3 and medium with manganese ions enhanced MEL-A production by Pseudozyma aphidis ZJUDM34.
glycolipid, biosurfactant, mannosylerythritol lipid-A, Pseudozyma aphidis, homologs, bioemulsifier
Structure type: monomer
Location inside paper: fig. 1, structure MEL-B, fig. 4
Trivial name: mannosylerythritol lipids (MELs)
Compound class: glycolipid, mannosylerythritol
Contained glycoepitopes: IEDB_114707,IEDB_137485,IEDB_144983,IEDB_152206,IEDB_983930,SB_44,SB_72
Methods: 13C NMR, 1H NMR, TLC, cell growth, GC–MS, LC–MS, HPLC–MS
Comments, role: Authors of the article could not determine at all which one fatty acid this structure contain. LIP is non-, mono-, di- or polyunsaturated fatty acid, number of carbon atoms 8-18
Related record ID(s): 46293, 46320, 46321, 46322, 46403
NCBI Taxonomy refs (TaxIDs): 84754,
5270
Show glycosyltransferases
There is only one chemically distinct structure:
Expand this record
Collapse this record
Fan LL, Dong YC, Fan YF, Zhang J, Chen QH
Production and identification of mannosylerythritol lipid-A homologs from the ustilaginomycetous yeast Pseudozyma aphidis ZJUDM34
Carbohydrate Research 392 (2014)
1-6
Pseudozyma aphidis ZJUDM34
(Ancestor NCBI TaxID 84754,
species name lookup)
Ustilago maydis
(NCBI TaxID 5270,
species name lookup)
Taxonomic group: fungi / Basidiomycota
(Phylum: Basidiomycota)
The structure was elucidated in this paperNCBI PubMed ID: 24814655Publication DOI: 10.1016/j.carres.2014.04.013Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: Chen QH <chenqh
zju.edu.cn>
Institutions: School of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou, China, Fuli Institute of Food Science, Zhejiang University, Hangzhou, China
Mannosylerythritol lipids (MELs) are mainly produced by strains of the genus Pseudozyma and by Ustilago maydis. These glycolipid biosurfactants exhibit not only excellent surface-active properties but also versatile bioactivities. Mannosylerythritol lipid-A (MEL-A) is worth investigating due to its self-assembling property. In this work, crude MELs were produced by resting Pseudozyma aphidis ZJUDM34 cells using different culture media. MEL-A fractions were isolated and identified using high-performance liquid chromatography combined with mass spectrometry (HPLC-MS) and gas chromatography combined with mass spectrometry (GC-MS). The results showed that MEL-A homologs had long unsaturated fatty acid chains, and the chain lengths range from C8 to C20. Nuclear magnetic resonance (NMR) was employed to confirm the chemical structures of the MEL-A homologs. Fermentation medium without NaNO3 and medium with manganese ions enhanced MEL-A production by Pseudozyma aphidis ZJUDM34.
glycolipid, biosurfactant, mannosylerythritol lipid-A, Pseudozyma aphidis, homologs, bioemulsifier
Structure type: monomer
Location inside paper: fig. 1, structure MEL-C, fig. 4
Contained glycoepitopes: IEDB_114707,IEDB_137485,IEDB_144983,IEDB_152206,IEDB_983930,SB_44,SB_72
Methods: 13C NMR, 1H NMR, TLC, cell growth, GC–MS, LC–MS, HPLC–MS
Comments, role: Authors of the article could not determine at all which one fatty acid this structure contain. LIP is non-, mono-, di- or polyunsaturated fatty acid, number of carbon atoms 8-18
Related record ID(s): 46293, 46319, 46321, 46322, 46403
NCBI Taxonomy refs (TaxIDs): 84754,
5270
Show glycosyltransferases
There is only one chemically distinct structure:
Expand this record
Collapse this record
Fan LL, Dong YC, Fan YF, Zhang J, Chen QH
Production and identification of mannosylerythritol lipid-A homologs from the ustilaginomycetous yeast Pseudozyma aphidis ZJUDM34
Carbohydrate Research 392 (2014)
1-6
Pseudozyma aphidis ZJUDM34
(Ancestor NCBI TaxID 84754,
species name lookup)
Ustilago maydis
(NCBI TaxID 5270,
species name lookup)
Taxonomic group: fungi / Basidiomycota
(Phylum: Basidiomycota)
The structure was elucidated in this paperNCBI PubMed ID: 24814655Publication DOI: 10.1016/j.carres.2014.04.013Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: Chen QH <chenqh
zju.edu.cn>
Institutions: School of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou, China, Fuli Institute of Food Science, Zhejiang University, Hangzhou, China
Mannosylerythritol lipids (MELs) are mainly produced by strains of the genus Pseudozyma and by Ustilago maydis. These glycolipid biosurfactants exhibit not only excellent surface-active properties but also versatile bioactivities. Mannosylerythritol lipid-A (MEL-A) is worth investigating due to its self-assembling property. In this work, crude MELs were produced by resting Pseudozyma aphidis ZJUDM34 cells using different culture media. MEL-A fractions were isolated and identified using high-performance liquid chromatography combined with mass spectrometry (HPLC-MS) and gas chromatography combined with mass spectrometry (GC-MS). The results showed that MEL-A homologs had long unsaturated fatty acid chains, and the chain lengths range from C8 to C20. Nuclear magnetic resonance (NMR) was employed to confirm the chemical structures of the MEL-A homologs. Fermentation medium without NaNO3 and medium with manganese ions enhanced MEL-A production by Pseudozyma aphidis ZJUDM34.
glycolipid, biosurfactant, mannosylerythritol lipid-A, Pseudozyma aphidis, homologs, bioemulsifier
Structure type: monomer
Location inside paper: fig. 1, structure MEL-D, fig. 4
Contained glycoepitopes: IEDB_114707,IEDB_137485,IEDB_144983,IEDB_152206,IEDB_983930,SB_44,SB_72
Methods: 13C NMR, 1H NMR, TLC, cell growth, GC–MS, LC–MS, HPLC–MS
Comments, role: Authors of the article could not determine at all which one fatty acid this structure contain. LIP is non-, mono-, di- or polyunsaturated fatty acid, number of carbon atoms 8-18
Related record ID(s): 46293, 46319, 46320, 46322, 46403
NCBI Taxonomy refs (TaxIDs): 84754,
5270
Show glycosyltransferases
NMR conditions: in CDCl3 at 298 K
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6
4,2 LIP
4,3 LIP
4 bDManp 99.7 69.4 71.3 66.1 71.7 62.8
xDEry-ol 63.2 71.8 69.9 73.1
1H NMR data:
Linkage Residue H1 H2 H3 H4 H5 H6
4,2 LIP
4,3 LIP
4 bDManp 4.7 5.5 4.9 4.4-4.6 3.5-3.6 4.3
xDEry-ol 3.6-3.7 3.7 3.6 3.6
1H/13C HSQC data:
Linkage Residue C1/H1 C2/H2 C3/H3 C4/H4 C5/H5 C6/H6
4,2 LIP
4,3 LIP
4 bDManp 99.7/4.7 69.4/5.5 71.3/4.9 66.1/4.4-4.6 71.7/3.5-3.6 62.8/4.3
xDEry-ol 63.2/3.6-3.7 71.8/3.7 69.9/3.6 73.1/3.6
1H NMR data:
| Linkage | Residue | H1 | H2 | H3 | H4 | H5 | H6 |
|---|
| 4,2 | LIP | |
| 4,3 | LIP | |
| 4 | bDManp | 4.7 | 5.5 | 4.9 | 4.4
4.6 | 3.5
3.6 | 4.3 |
| | xDEry-ol | 3.6
3.7 | 3.7 | 3.6 | 3.6 | |
|
13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 |
|---|
| 4,2 | LIP | |
| 4,3 | LIP | |
| 4 | bDManp | 99.7 | 69.4 | 71.3 | 66.1 | 71.7 | 62.8 |
| | xDEry-ol | 63.2 | 71.8 | 69.9 | 73.1 | |
|
There is only one chemically distinct structure:
Expand this record
Collapse this record
Fan LL, Dong YC, Fan YF, Zhang J, Chen QH
Production and identification of mannosylerythritol lipid-A homologs from the ustilaginomycetous yeast Pseudozyma aphidis ZJUDM34
Carbohydrate Research 392 (2014)
1-6
Pseudozyma aphidis ZJUDM34
(Ancestor NCBI TaxID 84754,
species name lookup)
Ustilago maydis
(NCBI TaxID 5270,
species name lookup)
Taxonomic group: fungi / Basidiomycota
(Phylum: Basidiomycota)
The structure was elucidated in this paperNCBI PubMed ID: 24814655Publication DOI: 10.1016/j.carres.2014.04.013Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: Chen QH <chenqh
zju.edu.cn>
Institutions: School of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou, China, Fuli Institute of Food Science, Zhejiang University, Hangzhou, China
Mannosylerythritol lipids (MELs) are mainly produced by strains of the genus Pseudozyma and by Ustilago maydis. These glycolipid biosurfactants exhibit not only excellent surface-active properties but also versatile bioactivities. Mannosylerythritol lipid-A (MEL-A) is worth investigating due to its self-assembling property. In this work, crude MELs were produced by resting Pseudozyma aphidis ZJUDM34 cells using different culture media. MEL-A fractions were isolated and identified using high-performance liquid chromatography combined with mass spectrometry (HPLC-MS) and gas chromatography combined with mass spectrometry (GC-MS). The results showed that MEL-A homologs had long unsaturated fatty acid chains, and the chain lengths range from C8 to C20. Nuclear magnetic resonance (NMR) was employed to confirm the chemical structures of the MEL-A homologs. Fermentation medium without NaNO3 and medium with manganese ions enhanced MEL-A production by Pseudozyma aphidis ZJUDM34.
glycolipid, biosurfactant, mannosylerythritol lipid-A, Pseudozyma aphidis, homologs, bioemulsifier
Structure type: monomer ; 737.1-961.5 [M+H]+
Location inside paper: fig. 1, structure MEL-A, Table III, fig. 4
Contained glycoepitopes: IEDB_114707,IEDB_137485,IEDB_144983,IEDB_152206,IEDB_983930,SB_44,SB_72
Methods: 13C NMR, 1H NMR, TLC, cell growth, GC–MS, LC–MS, HPLC–MS
Comments, role: LIP is non-, mono-, di- or polyunsaturated fatty acid, number of carbon atoms 8-18
Related record ID(s): 46293, 46319, 46320, 46321, 46403
NCBI Taxonomy refs (TaxIDs): 84754,
5270
Show glycosyltransferases
There is only one chemically distinct structure:
Expand this record
Collapse this record
Total list of record IDs on all result pages of the current query:
Execution: 10 sec
report error