Taxonomic group: fungi / Basidiomycota
(Phylum: Basidiomycota)
Organ / tissue: cell wall
The structure was elucidated in this paperPublication DOI: 10.1016/j.foodhyd.2013.09.023Journal NLM ID: 8701770Publisher: New York, NY: Elsevier
Correspondence: Cheung PCK <petercheung
cuhk.edu.hk>
Institutions: School of Life Sciences, The Chinese University of Hong Kong, Shatin, China, CUHK Shenzhen Research Institute, Shenzhen, China
The cell wall structure of mushroom sclerotium was investigated by a fractionation of its soluble cell wall polysaccharides followed by chemical, physico-chemical and microscopic analyses. The present results suggest that cell wall structure of Pleurotus tuber regium sclerotium contains three main layers: an outer layer of glycoproteins, a middle layer of hyper-branched glucans and an inner layer of complex between hyper-branched glucan and chitin. The structure of the hyper-branched glucans were elucidated by the methylation analysis to be composed of →1)-Glcp-(4→ linkages as the backbone with some →1)-Glcp(6→ linkages existed in the side chains, while some →1)-Glcp-(3→ linkages might exist in the backbone or side chains. SEC-MALLS analysis revealed that hyper-branched β-glucans had a M-w ranging from 1400000 to 5200000 g/mol and R.M.S. radius ranging from 26 nm to 38.5 nm. SEM and AFM further showed that hyper-branched beta-glucans were spherical in shape when dispersed in water.
Structure type: homopolymer
Location inside paper: p. 189, column 1, line 15
Trivial name: chitin
Compound class: O-polysaccharide, cell wall polysaccharide, glucan, polysaccharide, chitin
Contained glycoepitopes: IEDB_135813,IEDB_137340,IEDB_141807,IEDB_151531,IEDB_153212,IEDB_241099,IEDB_423114,IEDB_423150,SB_74,SB_85
Methods: methylation, IR, GC-MS, SDS-PAGE, extraction, cell growth, SEC-MALLS, precipitation, microscopic analysis
Comments, role: The article does not possess any information on chitin structure.
Related record ID(s): 40517, 43385, 46405, 101632, 118702, 139869, 143703, 145244, 147175, 149428, 149871, 149874
NCBI Taxonomy refs (TaxIDs): 716892Reference(s) to other database(s): GTC:G97099AY, CCSD:
46067, CBank-STR:5851, GenDB:KF905651
Show glycosyltransferases
There is only one chemically distinct structure:
Taxonomic group: fungi / Basidiomycota
(Phylum: Basidiomycota)
Organ / tissue: mycelium
The structure was elucidated in this paperPublication DOI: 10.1016/j.foodhyd.2013.09.023Journal NLM ID: 8701770Publisher: New York, NY: Elsevier
Correspondence: Cheung PCK <petercheung
cuhk.edu.hk>
Institutions: School of Life Sciences, The Chinese University of Hong Kong, Shatin, China, CUHK Shenzhen Research Institute, Shenzhen, China
The cell wall structure of mushroom sclerotium was investigated by a fractionation of its soluble cell wall polysaccharides followed by chemical, physico-chemical and microscopic analyses. The present results suggest that cell wall structure of Pleurotus tuber regium sclerotium contains three main layers: an outer layer of glycoproteins, a middle layer of hyper-branched glucans and an inner layer of complex between hyper-branched glucan and chitin. The structure of the hyper-branched glucans were elucidated by the methylation analysis to be composed of →1)-Glcp-(4→ linkages as the backbone with some →1)-Glcp(6→ linkages existed in the side chains, while some →1)-Glcp-(3→ linkages might exist in the backbone or side chains. SEC-MALLS analysis revealed that hyper-branched β-glucans had a M-w ranging from 1400000 to 5200000 g/mol and R.M.S. radius ranging from 26 nm to 38.5 nm. SEM and AFM further showed that hyper-branched beta-glucans were spherical in shape when dispersed in water.
Structure type: structural motif or average structure
Location inside paper: SCW
Contained glycoepitopes: IEDB_141806,IEDB_142488,IEDB_146664,IEDB_153543,IEDB_241101,IEDB_983931,SB_192
Methods: methylation, IR, GC-MS, SDS-PAGE, extraction, cell growth, SEC-MALLS, precipitation, microscopic analysis
Comments, role: 1-3-β-DGlcp could also be in a backbone
Related record ID(s): 46404
NCBI Taxonomy refs (TaxIDs): 716892Reference(s) to other database(s): GTC:G23353NT
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
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