Taxonomic group: fungi / Basidiomycota
(Phylum: Basidiomycota)
Organ / tissue: mycelium
The structure was elucidated in this paperNCBI PubMed ID: 24266379Publication DOI: 10.1615/IntJMedMushr.v15.i6.40Journal NLM ID: 100886202Publisher: New York, NY: Begell House, Inc.
Correspondence: Leon-Rivera I <ismaelr
uaem.mx>
Institutions: Facultad de Farmacia, Universidad Autonoma del Estado de Morelos, Morelos, México, Laboratorio de Enfermedades Neurodegenerativas, Instituto Nacional de Neurologia, MVS, Col. La Fama Distrito Federal, México, Instituto de Quimica, Universidad Nacional Autonoma de México, Circuito Exterior, Ciudad Universitaria, Coyoacan, Distrito Federal, México, Centro de Investigacion Biomédica del Sur, Instituto Mexicano del Seguro Social, Xochitepec, Morelos, México, Centro de Investigaciones Quimicas, Universidad Autonoma del Estado de Morelos, Col. Chamilpa Cuernavaca, Morelos, México, Centro de Investigaciones Biologicas, Universidad Autonoma del Estado de Morelos, Col. Chamilpa Cuernavaca, Morelos, México, Laboratorio Experimental de Neuropatologia, Instituto Nacional de Neurologia, MVS, Col. La Fama Distrito Federal, México
An oligosaccharide fraction isolated from the mycelium of the Lingzhi or Reishi medicinal mushroom Ganoderma lucidum (GLOS) was separated by size-exclusion chromatography. The chemical structure of GLOS consists of a disaccharide repeating unit [-4-β-1-Galf(1-6)-O-(β-Glcp)-1-]n (n=3,4). In addition, this study was undertaken to determine the possible anticonvulsant and neuroprotective effects of GLOS (10-80 mg/kg) on kainic acid (KA)-induced seizures. The behavioral alterations and histopathology of hippocampal neurons were studied. Our results show that GLOS inhibited convulsions in rats from KA-induced seizures, reduced the degeneration pattern in the CA3 region of rats, decreased astrocytic reactivity, and reduced the expression of IL-1β and TNF-α induced by KA. These results indicate a potential anticonvulsant and neuroprotective effects of GLOS.
oligosaccharide, Ganoderma lucidum, medicinal mushrooms, Lingzhi or Reishi mushroom, neuroprotective, anticonvulsant
Structure type: polymer chemical repeating unit ; n=3-4, 800-1300
Location inside paper: GLOS, p.560, p.559
Contained glycoepitopes: IEDB_136044,IEDB_137472,IEDB_141794,IEDB_142488,IEDB_146664,IEDB_190606,IEDB_983931,SB_165,SB_166,SB_187,SB_192,SB_195,SB_7,SB_88
Methods: 13C NMR, 1H NMR, NMR-2D, acid hydrolysis, HPLC, HPSEC, biological assay, immunohistochemistry, HR-MALDI-TOF-MS, anticonvulsant assay
Biological activity: complete preservation of hippocampal neurons was found in rats treated with GLOS (10–80 mg/kg) compared with abundant neuronal loss in CA3 region of rats and hilus after injection of kainic acid (10 mg/Kg)
Comments, role: erroneous galactose ringsize, right ringsize see p.559, column 2, paragraph 1
Related record ID(s): 45527
NCBI Taxonomy refs (TaxIDs): 5315Reference(s) to other database(s): GTC:G50347LH
Show glycosyltransferases
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6
6 bDGalp 106.3 73.7 72.0 84.1 73.8 60.1
bDGlcp 94.8 75.2 76.7 75.3 79.1 65.0
1H NMR data:
Linkage Residue H1 H2 H3 H4 H5 H6
6 bDGalp 5.41 3.56 3.86 4.05 3.85 3.67-3.83
bDGlcp 4.23 3.83 3.47 3.69 3.79 3.66-3.82
1H/13C HSQC data:
Linkage Residue C1/H1 C2/H2 C3/H3 C4/H4 C5/H5 C6/H6
6 bDGalp 106.3/5.41 73.7/3.56 72.0/3.86 84.1/4.05 73.8/3.85 60.1/3.67-3.83
bDGlcp 94.8/4.23 75.2/3.83 76.7/3.47 75.3/3.69 79.1/3.79 65.0/3.66-3.82
1H NMR data:
| Linkage | Residue | H1 | H2 | H3 | H4 | H5 | H6 |
|---|
| 6 | bDGalp | 5.41 | 3.56 | 3.86 | 4.05 | 3.85 | 3.67
3.83 |
| | bDGlcp | 4.23 | 3.83 | 3.47 | 3.69 | 3.79 | 3.66
3.82 |
|
13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 |
|---|
| 6 | bDGalp | 106.3 | 73.7 | 72.0 | 84.1 | 73.8 | 60.1 |
| | bDGlcp | 94.8 | 75.2 | 76.7 | 75.3 | 79.1 | 65.0 |
|
There is only one chemically distinct structure:
Found 
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