Taxonomic group: fungi / Ascomycota (Phylum: Ascomycota)
Organ / tissue: fruiting body
 
The structure was elucidated in this paper
NCBI PubMed ID: 24507284
Publication DOI: 10.1016/j.carbpol.2013.11.061
Journal NLM ID: 8307156
Publisher: Elsevier
Correspondence: Song L <tslyjnu.edu.cn>; Yu R <tyrmjnu.edu.cn>
Institutions: Biotechnological Institute of Chinese Materia Medica, Jinan University, Guangzhou, China, Department of Pharmacology, Jinan University, Guangzhou, China

A novel low-molecular-weight polysaccharide (CMP-1) with antioxidant, immunostimulatory and antitumor activities was isolated from the cultured Cordyceps militaris. The structure of CMP-1 was elucidated by a combination of physicochemical and instrumental analyses, and its average molecular weight was 4.3 kDa. The backbone of CMP-1 was composed of (1→4)-linked α-D-glucopyranosyl, (1→6)-linked β-D-glucopyranosyl and (1→4)-linked β-D-glucopyranosyl residues which branched at O-6. The branches consisted of (1→3)-linked α-L-rhamnopyranosyl terminated with (1→)-linked α-D-glucopyranosyl residues. The ultrastructure of CMP-1 was further investigated by scanning electron microscope (SEM). The antioxidant assays showed that CMP-1 exhibited free radical-scavenging effects, ferrous ions-chelating ability and reducing power. In vitro test revealed that CMP-1 could significantly stimulate the mouse splenocyte proliferation. The cytotoxicity assay showed that CMP-1 inhibited the proliferation of HT-29, HeLa, HepG2 and K562 cells, with the IC50 values of 137.66, 162.59, 176.29 and 364.01 μg/mL, respectively.

structure characterization, Antioxidant activity, antitumor activity, Immunostimulatory activity, Cordyceps militaris, low-molecular-weight polysaccharide

Structure type: structural motif or average structure ; 4300
Location inside paper: fig. 2, CMP-1, Table 3
The structure in this paper was incorrect:

← originally published structure   Show legend

Compound class: polysaccharide
Contained glycoepitopes: IEDB_135614,IEDB_136105,IEDB_140629,IEDB_141806,IEDB_142488,IEDB_144144,IEDB_144998,IEDB_146664,IEDB_158539,IEDB_225177,IEDB_241101,IEDB_420417,IEDB_420418,IEDB_420421,IEDB_423115,IEDB_857742,IEDB_885823,IEDB_983931,SB_192
 
Methods: 13C NMR, 1H NMR, periodate oxidation, GC-MS, GC, Smith degradation, GPC, HPAEC-PAD, statistical analysis, methylation analysis, reduction with NaBH4, cytotoxicity assay, GC-FID, phenol-sulfuric acid assay, HMBC, radical scavenging assay, SEM, HSQC, antioxidant activity assay, FT-IR, splenocyte proliferation assay, TFA hydrolysis, optical radical measurement
Biological activity: The antioxidant assays showed that CMP-1 exhibited DPPH, hydroxyl free radical-scavenging effects (IC50 of 1150 μg/mL (DPPH), 650 μg/mL (HO)), Fe2+ ions chelating ability (25-1600 μg/mL (57.9% of chelation for 1600 μg/mL) with IC50 of 550 μg/mL) and reducing power. In vitro test revealed that CMP-1 could significantly stimulate the mouse splenocyte proliferation. The cytotoxicity assay showed that CMP-1 inhibited the proliferation of HT-29, HeLa, HepG2 and K562 cells, with the IC50 values of 137.66, 162.59, 176.29 and 364.01 μg/mL, respectively
Comments, role: -4,6)bDGlcp(1- and -6)bDGlcp(1- units have erroneous α-anomeric configuration in Fig. 2; aLRhap is erroneously depicted as a β-anomer; NMR temperature was not specified; published erroneous NMR spectra were removed by CSDB staff due to multiple errors in the NMR assignment
3D data: ultrastructural data
 
NCBI Taxonomy refs (TaxIDs): 73501
Reference(s) to other database(s): GTC:G26970QK
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NMR conditions: in D2O        [as TSV]

13C NMR data: present in publication with incorrect assignment

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