Found 2 records.
Displayed records from 1 to 2
Expand all records
Collapse all records
Show all as text (SweetDB notation)
Show all graphically (SNFG notation)
Kim SY, Jeong H-S, Ahn S-W, Shin K-S
Prebiotic effects of structurally identified galacto-oligosaccharides produced by β-galactosidase from Aspergillus oryzae
Food Science and Biotechnology 23(3) (2014)
823–830
Aspergillus oryzae
(NCBI TaxID 5062,
species name lookup)
Taxonomic group: fungi / Ascomycota
(Phylum: Ascomycota)
The structure was elucidated in this paperPublication DOI: 10.1007/s10068-014-0111-7Journal NLM ID: 9816587Publisher: Seoul, Korea: Korean Society of Food Science and Technology
Correspondence: Shin K-S
Institutions: Nutrition Education, Graduate School of Education, Kyonggi University, Suwon, Gyeonggi, Korea, Technology Development Center, Ingredion Korea Incorporated, Icheon, Gyeonggi, Korea, Department of Food Science and Biotechnology, Kyonggi University, Suwon, Gyeonggi, Korea
Novel galacto-oligosaccharides were produced by β-galactosidase from Aspergillus oryzae using lactose, and their structural characteristics and prebiotic effects were examined. Highly purified oligosaccharide fraction (HP) was prepared from a crude one (low purified, LP) by gel-filtration on Biogel P-2 column, which was further purified into S1 and S2 fractions by prep-HPLC. S1 and S2 were comprised of galactose (Gal) and glucose (Glc) in the ratio of 2 to 1. ESI-MS-MS and methylation analysis indicated that S1 and S2 were trisaccharides with structures of β-D-Galp-(1,6)-β-D-Galp-(1,4)-β-D-Glcp and β-D-Galp-(1,3)-β-D-Galp-(1,4)-β-D-Glcp, respectively. Herein, LP and HP were used as the carbon sources for determining the prebiotic activity score of probiotics including Lactobacillus and Bifidobacterium species. LP and HP at 1% and 2% (w/v) were observed at all positive scores on several probiotics, especially, B. infantis ATCC 15697 at the 2% level of HP (p<0.05). Consequently, structurally identified trisaccharides of HP can significantly enhance the growth of B. infantis.
β-Galactosidase, probiotics, structural characteristics, galacto-oligosaccharide, prebiotic activity score
Structure type: oligomer ; 527 [M+Na]
Location inside paper: fraction S1, fig. 1(S1), fig. 3(S1), fig. 5(S1)
Contained glycoepitopes: IEDB_136044,IEDB_137472,IEDB_141794,IEDB_142487,IEDB_142488,IEDB_146664,IEDB_153201,IEDB_156493,IEDB_190606,IEDB_983931,SB_165,SB_166,SB_187,SB_192,SB_195,SB_6,SB_7,SB_88
Methods: GC-MS, ESI-MS, GC, HPLC, statistical analysis, methylation analysis, reduction with NaBH4, TFA hydrolysis, LS-MS, Hakamori method, prebiotic activity assay
Biological activity: trisaccharides (data given for 2% of HP in cultivation media) can enhance the growth of B. infantis ATCC 15697, Lb. plantarum KCTC 3104, Lb. casei ATCC 393, Lb. acidophilus ATCC 832, B. bifidum ATCC 29521 and E. coli KCTC 2441 with cell densities 4.29±0.03, 2.42±0.03, 2.75±0.03, 1.72±0.03, 0.73±0.01 and 0.41±0.01 respectively for 24h
Enzymes that release or process the structure: β-galactosidase
Synthetic data: enzymatic
Comments, role: galacto-oligosaccharide was produced by β-galactosidase from Aspergillus oryzae using lactose
Related record ID(s): 47552
NCBI Taxonomy refs (TaxIDs): 5062Reference(s) to other database(s): GTC:G60125IW
Show glycosyltransferases
There is only one chemically distinct structure:
Expand this record
Collapse this record
Kim SY, Jeong H-S, Ahn S-W, Shin K-S
Prebiotic effects of structurally identified galacto-oligosaccharides produced by β-galactosidase from Aspergillus oryzae
Food Science and Biotechnology 23(3) (2014)
823–830
Aspergillus oryzae
(NCBI TaxID 5062,
species name lookup)
Taxonomic group: fungi / Ascomycota
(Phylum: Ascomycota)
The structure was elucidated in this paperPublication DOI: 10.1007/s10068-014-0111-7Journal NLM ID: 9816587Publisher: Seoul, Korea: Korean Society of Food Science and Technology
Correspondence: Shin K-S
Institutions: Nutrition Education, Graduate School of Education, Kyonggi University, Suwon, Gyeonggi, Korea, Technology Development Center, Ingredion Korea Incorporated, Icheon, Gyeonggi, Korea, Department of Food Science and Biotechnology, Kyonggi University, Suwon, Gyeonggi, Korea
Novel galacto-oligosaccharides were produced by β-galactosidase from Aspergillus oryzae using lactose, and their structural characteristics and prebiotic effects were examined. Highly purified oligosaccharide fraction (HP) was prepared from a crude one (low purified, LP) by gel-filtration on Biogel P-2 column, which was further purified into S1 and S2 fractions by prep-HPLC. S1 and S2 were comprised of galactose (Gal) and glucose (Glc) in the ratio of 2 to 1. ESI-MS-MS and methylation analysis indicated that S1 and S2 were trisaccharides with structures of β-D-Galp-(1,6)-β-D-Galp-(1,4)-β-D-Glcp and β-D-Galp-(1,3)-β-D-Galp-(1,4)-β-D-Glcp, respectively. Herein, LP and HP were used as the carbon sources for determining the prebiotic activity score of probiotics including Lactobacillus and Bifidobacterium species. LP and HP at 1% and 2% (w/v) were observed at all positive scores on several probiotics, especially, B. infantis ATCC 15697 at the 2% level of HP (p<0.05). Consequently, structurally identified trisaccharides of HP can significantly enhance the growth of B. infantis.
β-Galactosidase, probiotics, structural characteristics, galacto-oligosaccharide, prebiotic activity score
Structure type: oligomer ; 527 [M+Na]
Location inside paper: fraction S2, fig. 1(S2), fig. 3(S2), fig. 5(S2)
Contained glycoepitopes: IEDB_136044,IEDB_137472,IEDB_141794,IEDB_142487,IEDB_142488,IEDB_146664,IEDB_190606,IEDB_983931,SB_165,SB_166,SB_187,SB_192,SB_195,SB_6,SB_7,SB_88
Methods: GC-MS, ESI-MS, GC, HPLC, statistical analysis, methylation analysis, reduction with NaBH4, TFA hydrolysis, LS-MS, Hakamori method, prebiotic activity assay
Biological activity: trisaccharides (data given for 2% of HP in cultivation media) can enhance the growth of B. infantis ATCC 15697, Lb. plantarum KCTC 3104, Lb. casei ATCC 393, Lb. acidophilus ATCC 832, B. bifidum ATCC 29521 and E. coli KCTC 2441 with cell densities 4.29±0.03, 2.42±0.03, 2.75±0.03, 1.72±0.03, 0.73±0.01 and 0.41±0.01 respectively for 24h
Enzymes that release or process the structure: β-galactosidase
Synthetic data: enzymatic
Comments, role: galacto-oligosaccharide was produced by β-galactosidase from Aspergillus oryzae using lactose
Related record ID(s): 47551
NCBI Taxonomy refs (TaxIDs): 5062Reference(s) to other database(s): GTC:G45796AX
Show glycosyltransferases
There is only one chemically distinct structure:
Expand this record
Collapse this record
Total list of record IDs on all result pages of the current query:
Execution: 4 sec
report error