Taxonomic group: fungi / Ascomycota
(Phylum: Ascomycota)
NCBI PubMed ID: 25789604Publication DOI: 10.1089/jmf.2014.3215Journal NLM ID: 9812512Publisher: Larchmont, NY: Mary Ann Liebert, Inc.
Correspondence: Hwang GS <seoul
gachon.ac.kr>
Institutions: Laboratory of Cell Differentiation Research, College of Korean Medicine, Gachon University, Seongnam, Korea, Gachon Institute of Pharmaceutical Sciences, College of Pharmacy, Gachon University, Incheon, Korea
Cordyceps militaris is a medicinal mushroom and its bioactive compound, cordycepin, is reported to have many pharmacological activities. The goal of this study was to investigate the effects of C. militaris extract (CME) and cordycepin on osteoclast differentiation in vitro and on an inflammatory bone loss in vivo. In RAW 264.7 cells, CME and cordycepin showed dose-dependent inhibition of receptor activator of the nuclear factor kappa B (NF-κB) ligand (RANKL)-induced osteoclast differentiation by TRAP (tartrate-resistant acid phosphatase) staining. Moreover, the mRNA expression of osteoclastogenesis-related genes (TRAP, cathepsin K, MMP-9, and NFATc1) was also inhibited by CME and cordycepin. Also, cordycepin significantly inhibited RANKL-induced phosphorylation of p38 and NF-κB, but not that of other members of mitogen-activated protein kinase families. To examine the effect of CME on bone loss in vivo, we used a mouse model of lipopolysaccharide (LPS)-mediated bone loss. Micro-CT analysis of the femurs showed that LPS treatment caused bone loss. However, bone loss was significantly attenuated in mice treated with CME. These results suggest that cordycepin or/and CME have inhibitory effects on osteoclast differentiation in vitro and that they suppress inflammatory bone loss in vivo
osteoclast differentiation, Cordyceps militaris, cordycepin, TRAP
Structure type: monomer ; 252
Location inside paper: p. 448, right column, paragraph 1
Trivial name: cordycepin
Compound class: nucleoside
Methods: PCR, Western blotting, biological assays, HPLC, extraction, cell viability assay, sonication, centrifugation, TRAP staining
Biological activity: the formation of multinucleated osteoclasts was inhibited by cordycepin dose dependently. Treatment of RAW 264.7 cells with cordycepin (0.1-10 lg/mL) for 24 h did not cause any significant change in cell viability. Cordycepin markedly suppressed the mRNA expressions of cathepsin K,MMP-9, and NFATc1 induced by RANKL for 4 days. NF-jB activation was enhanced by RANKL, but treatment with cordycepin suppressed that activation in a dose-dependent manner
Related record ID(s): 49889, 49890, 49902, 49903, 49906, 49914, 49917, 49920, 49928, 49941, 49948, 49951, 50772, 50774, 50784, 50785, 50786, 50797, 50802, 50804, 50807, 50809, 50814, 50817
NCBI Taxonomy refs (TaxIDs): 73501
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
report error