Taxonomic group: fungi / Ascomycota
(Phylum: Ascomycota)
Publication DOI: 10.1016/j.meteno.2015.09.001Journal NLM ID: 101642117Publisher: Amsterdam: Elsevier
Correspondence: Mortensen UH <um
bio.dtu.dk>
Institutions: Department of Systems Biology, Technical University of Denmark, Lyngby, Denmark
The yeast Saccharomyces cerevisiae is a widely used eukaryotic model organism and a key cell factory for production of biofuels and wide range of chemicals. From the broad palette of available yeast strains, the most popular are those derived from laboratory strain S288c and the industrially relevant CEN.PK strain series. Importantly, in recent years these two strains have been subjected to comparative “-omics” analyzes pointing out significant genotypic and phenotypic differences. It is therefore possible that the two strains differ significantly with respect to their potential as cell factories for production of specific compounds. To examine this possibility, we have reconstructed a de novo vanillin-β-glucoside pathway in an identical manner in S288c and CEN.PK strains. Characterization of the two resulting strains in two standard conditions revealed that the S288c background strain produced up to 10-fold higher amounts of vanillin-β-glucoside compared to CEN.PK. This study demonstrates that yeast strain background may play a major role in the outcome of newly developed cell factories for production of a given product
yeast, cell factory, strain choice, heterologous production, vanillin-glucoside, shikimate pathway
Structure type: monomer
Location inside paper: Fig. 1, VG
Compound class: glycoside
Contained glycoepitopes: IEDB_142488,IEDB_146664,IEDB_983931,SB_192
Methods: PCR, DNA techniques, MS, HPLC, UV, cell growth, ion-pair chromatography
Enzymes that release or process the structure: 3-dehydroshikimate dehydratase, aromatic carboxylic acid reductase, phosphopantetheine transferase, O-methyltransferase, UDP-glycosyltransferase
Synthetic data: enzymatic in vivo
NCBI Taxonomy refs (TaxIDs): 4932
Show glycosyltransferases
There is only one chemically distinct structure:
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