Taxonomic group: fungi / Ascomycota
(Phylum: Ascomycota)
Organ / tissue: fruiting bodies
The structure was elucidated in this paperNCBI PubMed ID: 30551572Publication DOI: 10.3390/ijms19124027Journal NLM ID: 101092791Publisher: Basel, Switzerland: MDPI
Correspondence: Xu N <doctorserena
163.com>; Lu Y <luyi920127
163.com>; Hou JM <houjumin0511
126.com>; Liu C <liuchaocarrol
163.com>; Sun YH <sunyonghaijida
163.com>
Institutions: College of Food Science and Engineering, Jilin University, Changchun, China, Jilin Provincial Key Laboratory of Animal Embryo Engineering, Jilin University, Changchun, China, College of Food Science and Engineering, Changchun University, Changchun, China, School of Food Engineering, Jilin Agriculture Science and Technology College, Jilin, China
Morchella conica Pers. (M. conica) has been used both as a medical and edible mushroom and possesses antimicrobial properties and antioxidant activities. However, the antioxidant properties of polysaccharides purified from M. conica have not been studied. The aim of this study was to investigate the in vitro antioxidant properties of a polysaccharide NMCP-2 (neutral M. conica polysaccharides-2) purified from M. conica, as determined by radical scavenging assay and H2O2-induced oxidative stress in HEK 293T cells. Results showed that NMCP-2 with an average molecular weight of 48.3 kDa possessed a much stronger chelating ability on ferrous ions and a higher ability to scavenge radical scavenging 2,2-diphenyl-1-picrylhydrazyl (DPPH) than the other purified fraction of NMCP-1 from M. conica. Moreover, 3-(4, 5-Dimethylthiazol-2-yl)-2, 5-diphenyltetra-zolium bromide (MTT) assay showed that NMCP-2 dose-dependently preserved cell viability of H2O2-induced cells. The NMCP-2 pretreated group reduced the generation of reactive oxygen species (ROS) content and increased the mitochondria membrane potential (MMP) levels. In addition, Hoechst 33342 staining revealed cells treated with NMCP-2 declined nuclear condensation. Ultrastructural observation revealed that NMCP-2 pretreatment alleviated the ruptured mitochondria when exposed to H2O2. Furthermore, western blot analysis showed that NMCP-2 prevented significant downregulation of the protein expression of Bax, cleaved caspases 3, and upregulated Bcl-2 levels. These results suggest the protective effects of NMCP-2 against H2O2-induced injury in HEK 293T cells. NMCP-2 could be used as a natural antioxidant of functional foods and natural drugs.
polysaccharides, oxidative stress, Morchella conica
Structure type: fragment of a bigger structure
Location inside paper: fig. 5(top), table 1, NMCP-2
Contained glycoepitopes: IEDB_114701,IEDB_136044,IEDB_137472,IEDB_140629,IEDB_141794,IEDB_142488,IEDB_144998,IEDB_146664,IEDB_167188,IEDB_174332,IEDB_190606,IEDB_983931,SB_165,SB_166,SB_187,SB_192,SB_195,SB_7,SB_88
Methods: 13C NMR, 1H NMR, GC-MS, Western blotting, acetylation, statistical analysis, DEAE, cell viability assay, precipitation, phenol-sulfuric acid assay, HMBC, radical scavenging assay, TEM, centrifugation, HSQC, TFA hydrolysis, FT-IR< HPLC, fluorescent mocroscopy
Biological activity: increases viability of HEK 293T cells in dose-dependent manner(93.77% for 25 μg/mL); showed a significant effect for preventing cell apoptosis at 25 μg/mL
Comments, role: molecular mass of the whole polysaccharide is 48300 Da
Related record ID(s): 49119
NCBI Taxonomy refs (TaxIDs): 5194Reference(s) to other database(s): GTC:G38411QS
Show glycosyltransferases
NMR conditions: in D2O
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6
4,6,4 bDGalp 103.43 73.29 76.67 76.38 ? ?
4,6 bDXylp 101.30 79.61 75.69 77.01 66.86
4 aDGlcp 98.22 77.26 74.15 70.35 71.16 66.34
aDGlcp 99.30 71.16 71.90 76.48 75.31 61.02
1H NMR data:
Linkage Residue H1 H2 H3 H4 H5 H6
4,6,4 bDGalp 4.54 3.33 3.56 3.72 ? ?
4,6 bDXylp 4.56 3.56 3.69 3.85 4.13
4 aDGlcp 5.02 3.60 3.73 3.56 3.96 4.05
aDGlcp 5.12 3.59 3.93 3.81 3.56 3.96
1H/13C HSQC data:
Linkage Residue C1/H1 C2/H2 C3/H3 C4/H4 C5/H5 C6/H6
4,6,4 bDGalp 103.43/4.54 73.29/3.33 76.67/3.56 76.38/3.72 ?/? ?/?
4,6 bDXylp 101.30/4.56 79.61/3.56 75.69/3.69 77.01/3.85 66.86/4.13
4 aDGlcp 98.22/5.02 77.26/3.60 74.15/3.73 70.35/3.56 71.16/3.96 66.34/4.05
aDGlcp 99.30/5.12 71.16/3.59 71.90/3.93 76.48/3.81 75.31/3.56 61.02/3.96
1H NMR data:
| Linkage | Residue | H1 | H2 | H3 | H4 | H5 | H6 |
|---|
| 4,6,4 | bDGalp | 4.54 | 3.33 | 3.56 | 3.72 | ? | ? |
| 4,6 | bDXylp | 4.56 | 3.56 | 3.69 | 3.85 | 4.13 | |
| 4 | aDGlcp | 5.02 | 3.60 | 3.73 | 3.56 | 3.96 | 4.05 |
| | aDGlcp | 5.12 | 3.59 | 3.93 | 3.81 | 3.56 | 3.96 |
|
13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 |
|---|
| 4,6,4 | bDGalp | 103.43 | 73.29 | 76.67 | 76.38 | ? | ? |
| 4,6 | bDXylp | 101.30 | 79.61 | 75.69 | 77.01 | 66.86 | |
| 4 | aDGlcp | 98.22 | 77.26 | 74.15 | 70.35 | 71.16 | 66.34 |
| | aDGlcp | 99.30 | 71.16 | 71.90 | 76.48 | 75.31 | 61.02 |
|
The spectrum also has 2 signals at unknown positions (not plotted). |
There is only one chemically distinct structure:
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