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Taxonomic group: fungi / Ascomycota (Phylum: Ascomycota)
Organ / tissue: fruiting bodies

The structure was elucidated in this paper
NCBI PubMed ID: 30551572
Publication DOI: 10.3390/ijms19124027
Journal NLM ID: 101092791
Publisher: Basel, Switzerland: MDPI
Correspondence: Xu N <doctorserena

163.com>; Lu Y <luyi920127

163.com>; Hou JM <houjumin0511

126.com>; Liu C <liuchaocarrol

163.com>; Sun YH <sunyonghaijida

163.com>
Institutions: College of Food Science and Engineering, Jilin University, Changchun, China, Jilin Provincial Key Laboratory of Animal Embryo Engineering, Jilin University, Changchun, China, College of Food Science and Engineering, Changchun University, Changchun, China, School of Food Engineering, Jilin Agriculture Science and Technology College, Jilin, China

Morchella conica Pers. (M. conica) has been used both as a medical and edible mushroom and possesses antimicrobial properties and antioxidant activities. However, the antioxidant properties of polysaccharides purified from M. conica have not been studied. The aim of this study was to investigate the in vitro antioxidant properties of a polysaccharide NMCP-2 (neutral M. conica polysaccharides-2) purified from M. conica, as determined by radical scavenging assay and H2O2-induced oxidative stress in HEK 293T cells. Results showed that NMCP-2 with an average molecular weight of 48.3 kDa possessed a much stronger chelating ability on ferrous ions and a higher ability to scavenge radical scavenging 2,2-diphenyl-1-picrylhydrazyl (DPPH) than the other purified fraction of NMCP-1 from M. conica. Moreover, 3-(4, 5-Dimethylthiazol-2-yl)-2, 5-diphenyltetra-zolium bromide (MTT) assay showed that NMCP-2 dose-dependently preserved cell viability of H2O2-induced cells. The NMCP-2 pretreated group reduced the generation of reactive oxygen species (ROS) content and increased the mitochondria membrane potential (MMP) levels. In addition, Hoechst 33342 staining revealed cells treated with NMCP-2 declined nuclear condensation. Ultrastructural observation revealed that NMCP-2 pretreatment alleviated the ruptured mitochondria when exposed to H2O2. Furthermore, western blot analysis showed that NMCP-2 prevented significant downregulation of the protein expression of Bax, cleaved caspases 3, and upregulated Bcl-2 levels. These results suggest the protective effects of NMCP-2 against H2O2-induced injury in HEK 293T cells. NMCP-2 could be used as a natural antioxidant of functional foods and natural drugs.

polysaccharides, oxidative stress, Morchella conica

Structure type: fragment of a bigger structure
Location inside paper: fig. 5(top), table 1, NMCP-2
Contained glycoepitopes: IEDB_114701,IEDB_136044,IEDB_137472,IEDB_140629,IEDB_141794,IEDB_142488,IEDB_144998,IEDB_146664,IEDB_167188,IEDB_174332,IEDB_190606,IEDB_983931,SB_165,SB_166,SB_187,SB_192,SB_195,SB_7,SB_88

Methods: 13C NMR, 1H NMR, GC-MS, Western blotting, acetylation, statistical analysis, DEAE, cell viability assay, precipitation, phenol-sulfuric acid assay, HMBC, radical scavenging assay, TEM, centrifugation, HSQC, TFA hydrolysis, FT-IR< HPLC, fluorescent mocroscopy
Biological activity: increases viability of HEK 293T cells in dose-dependent manner(93.77% for 25 μg/mL); showed a significant effect for preventing cell apoptosis at 25 μg/mL
Comments, role: molecular mass of the whole polysaccharide is 48300 Da

Related record ID(s): 49119
NCBI Taxonomy refs (TaxIDs): 5194
Reference(s) to other database(s): GTC:G38411QS
Show glycosyltransferases

NMR conditions: in D2O [as TSV]

13C NMR data:
Linkage	Residue	C1	C2	C3	C4	C5	C6
4,6,4	bDGalp	103.43	73.29	76.67	76.38	?	?
4,6	bDXylp	101.30	79.61	75.69	77.01	66.86
4	aDGlcp	98.22	77.26	74.15	70.35	71.16	66.34
	aDGlcp	99.30	71.16	71.90	76.48	75.31	61.02


1H NMR data:
Linkage	Residue	H1	H2	H3	H4	H5	H6
4,6,4	bDGalp	4.54	3.33	3.56	3.72	?	?
4,6	bDXylp	4.56	3.56	3.69	3.85	4.13
4	aDGlcp	5.02	3.60	3.73	3.56	3.96	4.05
	aDGlcp	5.12	3.59	3.93	3.81	3.56	3.96


1H/13C HSQC data:
Linkage	Residue	C1/H1	C2/H2	C3/H3	C4/H4	C5/H5	C6/H6
4,6,4	bDGalp	103.43/4.54	73.29/3.33	76.67/3.56	76.38/3.72	?/?	?/?
4,6	bDXylp	101.30/4.56	79.61/3.56	75.69/3.69	77.01/3.85	66.86/4.13
4	aDGlcp	98.22/5.02	77.26/3.60	74.15/3.73	70.35/3.56	71.16/3.96	66.34/4.05
	aDGlcp	99.30/5.12	71.16/3.59	71.90/3.93	76.48/3.81	75.31/3.56	61.02/3.96

¹H NMR data:

Linkage	Residue	H1	H2	H3	H4	H5	H6
4,6,4	bDGalp	4.54	3.33	3.56	3.72	?	?
4,6	bDXylp	4.56	3.56	3.69	3.85	4.13
4	aDGlcp	5.02	3.60	3.73	3.56	3.96	4.05
	aDGlcp	5.12	3.59	3.93	3.81	3.56	3.96

¹³C NMR data:

Linkage	Residue	C1	C2	C3	C4	C5	C6
4,6,4	bDGalp	103.43	73.29	76.67	76.38	?	?
4,6	bDXylp	101.30	79.61	75.69	77.01	66.86
4	aDGlcp	98.22	77.26	74.15	70.35	71.16	66.34
	aDGlcp	99.30	71.16	71.90	76.48	75.31	61.02

The spectrum also has 2 signals at unknown positions (not plotted).

There is only one chemically distinct structure:

[*]O[C@H]1[C@@H](CO)O[C@@H](O[C@@H]2CO[C@@H](OC[C@H]3O[C@H](O[C@@H]4[C@@H](CO)O[C@H]([*])[C@H](O)[C@H]4O)[C@H](O)[C@@H](O)[C@@H]3O)[C@H](O)[C@H]2O)[C@H](O)[C@H]1O

SVG MW SMILES 3D mol Ionize

163.com>; Lu Y <luyi920127

163.com>; Hou JM <houjumin0511

126.com>; Liu C <liuchaocarrol

163.com>; Sun YH <sunyonghaijida

Morchella conica Pers. (M. conica) has been used both as a medical and edible mushroom and possesses antimicrobial properties and antioxidant activities. However, the antioxidant properties of polysaccharides purified from M. conica have not been studied. The aim of this study was to investigate the in vitro antioxidant properties of a polysaccharide NMCP-2 (neutral M. conica polysaccharides-2) purified from M. conica, as determined by radical scavenging assay and H2O2-induced oxidative stress in HEK 293T cells. Results showed that NMCP-2 with an average molecular weight of 48.3 kDa possessed a much stronger chelating ability on ferrous ions and a higher ability to scavenge radical scavenging 2,2-diphenyl-1-picrylhydrazyl (DPPH) than the other purified fraction of NMCP-1 from M. conica. Moreover, 3-(4, 5-Dimethylthiazol-2-yl)-2, 5-diphenyltetra-zolium bromide (MTT) assay showed that NMCP-2 dose-dependently preserved cell viability of H2O2-induced cells. The NMCP-2 pretreated group reduced the generation of reactive oxygen species (ROS) content and increased the mitochondria membrane potential (MMP) levels. In addition, Hoechst 33342 staining revealed cells treated with NMCP-2 declined nuclear condensation. Ultrastructural observation revealed that NMCP-2 pretreatment alleviated the ruptured mitochondria when exposed to H₂O₂. Furthermore, western blot analysis showed that NMCP-2 prevented significant downregulation of the protein expression of Bax, cleaved caspases 3, and upregulated Bcl-2 levels. These results suggest the protective effects of NMCP-2 against H2O2-induced injury in HEK 293T cells. NMCP-2 could be used as a natural antioxidant of functional foods and natural drugs.

polysaccharides, oxidative stress, Morchella conica

Structure type: fragment of a bigger structure
Location inside paper: fig. 5(bottom), table 1, NMCP-2
Contained glycoepitopes: IEDB_130701,IEDB_137485,IEDB_1394182,IEDB_140629,IEDB_142488,IEDB_144983,IEDB_144998,IEDB_146664,IEDB_152206,IEDB_983930,IEDB_983931,SB_192,SB_44,SB_67,SB_72

Methods: 13C NMR, 1H NMR, GC-MS, Western blotting, HPLC, acetylation, statistical analysis, DEAE, cell viability assay, precipitation, phenol-sulfuric acid assay, HMBC, radical scavenging assay, TEM, centrifugation, HSQC, FT-IR, TFA hydrolysis, fluorescent mocroscopy
Biological activity: increases viability of HEK 293T cells in dose-dependent manner(93.77% for 25 μg/mL); showed a significant effect for preventing cell apoptosis at 25 μg/mL
Comments, role: molecular mass of the whole polysaccharide is 48300 Da

Related record ID(s): 49118
NCBI Taxonomy refs (TaxIDs): 5194
Reference(s) to other database(s): GTC:G87133XC
Show glycosyltransferases

NMR conditions: in D2O at 298 K [as TSV]

13C NMR data:
Linkage	Residue	C1	C2	C3	C4	C5	C6
3,6	aDGlcp	98.22	77.26	74.15	70.35	71.16	66.34
3,4,4	aDManp	102.22	70.05	69.87	66.86	73.29	60.86
3,4	aDGlcp	99.30	71.16	71.90	76.48	75.31	61.02
3	aDGlcp	96.24	72.54	73.15	77.73	?	63.33
	b?Manp	101.15	71.16	74.31	67.82	76.08	61.55


1H NMR data:
Linkage	Residue	H1	H2	H3	H4	H5	H6
3,6	aDGlcp	5.02	3.60	3.73	3.56	3.96	4.05
3,4,4	aDManp	4.98	4.00	3.72	3.57	3.69	3.69
3,4	aDGlcp	5.12	3.59	3.93	3.81	3.56	3.96
3	aDGlcp	5.08	?	3.96	3.69	?	3.69
	b?Manp	?	4.13	3.96	3.69	3.42	3.81


1H/13C HSQC data:
Linkage	Residue	C1/H1	C2/H2	C3/H3	C4/H4	C5/H5	C6/H6
3,6	aDGlcp	98.22/5.02	77.26/3.60	74.15/3.73	70.35/3.56	71.16/3.96	66.34/4.05
3,4,4	aDManp	102.22/4.98	70.05/4.00	69.87/3.72	66.86/3.57	73.29/3.69	60.86/3.69
3,4	aDGlcp	99.30/5.12	71.16/3.59	71.90/3.93	76.48/3.81	75.31/3.56	61.02/3.96
3	aDGlcp	96.24/5.08	72.54/?	73.15/3.96	77.73/3.69	?/?	63.33/3.69
	b?Manp	101.15/?	71.16/4.13	74.31/3.96	67.82/3.69	76.08/3.42	61.55/3.81

¹H NMR data:

Linkage	Residue	H1	H2	H3	H4	H5	H6
3,6	aDGlcp	5.02	3.60	3.73	3.56	3.96	4.05
3,4,4	aDManp	4.98	4.00	3.72	3.57	3.69	3.69
3,4	aDGlcp	5.12	3.59	3.93	3.81	3.56	3.96
3	aDGlcp	5.08	?	3.96	3.69	?	3.69
	b?Manp	?	4.13	3.96	3.69	3.42	3.81

¹³C NMR data:

Linkage	Residue	C1	C2	C3	C4	C5	C6
3,6	aDGlcp	98.22	77.26	74.15	70.35	71.16	66.34
3,4,4	aDManp	102.22	70.05	69.87	66.86	73.29	60.86
3,4	aDGlcp	99.30	71.16	71.90	76.48	75.31	61.02
3	aDGlcp	96.24	72.54	73.15	77.73	?	63.33
	b?Manp	101.15	71.16	74.31	67.82	76.08	61.55

The spectrum also has 1 signal at unknown position (not plotted).

There are 2 chemically distinct structures. Please, select:

[*]OC[C@H]1O[C@H](OC[C@H]2O[C@H](O[C@@H]3[C@H](O)[C@H]([*])O[C@H](CO)[C@H]3O)[C@H](O)[C@@H](O)[C@@H]2O[C@H]2O[C@H](CO)[C@@H](O[C@H]3O[C@H](CO)[C@@H](O)[C@H](O)[C@@H]3O)[C@H](O)[C@H]2O)[C@H](O)[C@@H](O)[C@@H]1O

SVG MW SMILES 3D mol Ionize