Zhao DQ, Ding X, Hou YL, Hou WR, Liu L, Xu T, Yang DN Structural characterization, immune regulation and antioxidant activity of a new heteropolysaccharide from Cantharellus cibarius Fr International Journal of Molecular Medicine41(5) (2018)
2744-2754
The structure was elucidated in this paper NCBI PubMed ID:29393398 Publication DOI:10.3892/ijmm.2018.3450 Journal NLM ID:9810955 Publisher: Athens, Greece: D.A. Spandidos Correspondence: Hou YL <starthlh126.com> Institutions: College of Environmental Science and Engineering, China West Normal University, Nanchong, China, Key Laboratory of Southwest China Wildlife Resources Conservation (Ministry of Education), College of Life Sciences, Nanchong, China, School of Basic Medical Sciences, Southwest Medical University, Luzhou, China
A new heteropolysaccharide was extracted and purified from the fruiting bodies of Cantharellus cibarius Fr. The Cantharellus cibarius Fr. polysaccharide (CC-1) had a molecular weight of 61,056 kDa and was mainly formed of the glucose and xylose at ratio of 5:1. Structure identification of CC-1 was analysed by a combined application of total hydrolysis, high performance liquid chromatography (HPLC), methylation analysis, gas chromatography-mass spectrometry (GC-MS), infrared (IR) spectra and nuclear magnetic resonance (NMR) spectroscopy. The experimental results showed that CC-1 had a backbone of 1,4-linked--D-glucose which branched at O-6 and the branches were mainly composed of 61)--D-xylopyranose residue. CC-1 exhibited significant in vitro antioxidant effect and proliferation effect of immune cells. The activity study showed CC-1 has ability to clear the ABTS(+) free radical and DPPH- free radical in a certain range of concentration. The proliferation activity of the immune cells showed that the proliferation effect on B cells was very significant (P<0.001) in the concentration of 0.625-80 mg/ml; and the effect of T cell proliferation was also very significant (P<0.001) in the concentration of 5-20 mg/ml. The result of this study introduced Cantharellus cibarius Fr. as a possible valuable source in exhibiting unique immunoregulatory and antioxidant properties.
NCBI PubMed ID:8434016 Publication DOI:10.1073/pnas.90.4.1551 Journal NLM ID:7505876 Publisher: National Academy of Sciences Institutions: Laboratorium voor Genetica, Universiteit Gent, Gent, Belgium
Azorhizobium caulinodans strain ORS571 is a symbiont of the tropical legume Sesbania rostrata. Upon nod gene induction with naringenin, strain ORS571 secretes into the culture medium Nod factors that morphologically change the host plant--in particular, deformed root hairs (Hai/Had) and meristematic foci are formed at the basis of lateral roots. The latter infrequently develop further into nodule-like structures. The azorhizobial Nod factors are chitin tetramers or pentamers, N-acylated at the nonreducing-end glucosamine with either vaccenic acid (C18:1) or stearic acid (C18:0). They, thus, resemble the previously described Nod factors from (brady)rhizobia. The backbone lipooligosaccharide is substituted with unusual modifications, presumably involved in host-specificity determination. There is a D-arabinose branch on the reducing end and an N-methyl and O-carbamoyl substitution on the nonreducing end of the oligosaccharide chain. The previously identified nod gene nolK may be involved in the synthesis of a D-arabinose derivative. The nodS gene product is probably responsible for the N-methylation of Nod factors.
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(later renamed to: Cantharellus cibarius)
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