Taxonomic group: fungi / Basidiomycota
(Phylum: Basidiomycota)
Organ / tissue: fruiting bodies
The structure was elucidated in this paperNCBI PubMed ID: 30590151Publication DOI: 10.1016/j.ijbiomac.2018.12.216Journal NLM ID: 7909578Publisher: Butterworth-Heinemann
Correspondence: Hou YL <starthlh
126.com>
Institutions: Key Laboratory of Southwest China Wildlife Resources Conservation, College of Life Sciences, China West Normal University, Nanchong, China, College of Environmental Science and Engineering, China West Normal University, Nanchong, China, Nanchong Academy of Agricultural Sciences, Nongke Road, Nanchong, China
A new water-soluble polysaccharide named BSF-X was extracted and purified from the fruiting bodies of Boletus speciosus Frost which had a molecular weight of 141,309 Da. The structure identification results showed that BSF-X was mainly composed of beta-D-glucose and alpha-D-galactose. BSF-X had a backbone of 1, 4-linked beta-D-glucose of which branches were mainly composed of two 1, 6-linked alpha-D-galactose residue and a 4-linked beta-D-glucose at the end of the branches. Antitumor activities results showed that BSF-X could inhibit the proliferation of L929 cells in vitro and S180 tumor cells in vivo. Immunoregulatory activities results showed that BSF-X could promote the proliferation of T cells, B cells and macrophages by promoting the cells into S phase from G0/G1 phase. Polysaccharide BSF-X can also enhance the phagocytes is and cytokine secretion of macrophages. This study introduced the new polysaccharide BSF-X as a valuable source which exhibit unique antitumor and immunoregulatory properties.
biological activities, Boletus speciosus Frost, structure identification, fungi polysaccharides
Structure type: structural motif or average structure ; 141309
Location inside paper: fig. 2(E), table 2, fig. 1(D, E), BSF-X
Compound class: EPS, glucan
Contained glycoepitopes: IEDB_134624,IEDB_136906,IEDB_137472,IEDB_140529,IEDB_141794,IEDB_142488,IEDB_146664,IEDB_151528,IEDB_167069,IEDB_190606,IEDB_983931,SB_163,SB_192,SB_7
Methods: 13C NMR, 1H NMR, GC-MS, ELISA, UV, DEAE, dialysis, determination of NO production, HPGPC, HMBC, antitumor activity assay, HMQC, COSY, FT-IR, TFA hydrolysis, western blotting, extraction precipitation
Biological activity: antitumor effect: inhibition rate of 40 mg/kg in mice 61.35%; inhibits proliferation of mouse fibroblasts(L929 cell line): inhibition rate: 38.15% at 20 μg/mL; promotes T cells proliferation: 76% at 20 μg/mL;
Comments, role: degree of branching differs among strains; published erroneous NMR spectra were removed by CSDB staff due to multiple errors in the NMR assignment
NCBI Taxonomy refs (TaxIDs): 374761Reference(s) to other database(s): GTC:G88834OF
Show glycosyltransferases
NMR conditions: in D2O
[as TSV]
13C NMR data: present in publication with incorrect assignment
|
There is only one chemically distinct structure:
Found 
report error