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Taxonomic group: fungi / Basidiomycota (Phylum: Basidiomycota)
Organ / tissue: fruiting bodies

The structure was elucidated in this paper
NCBI PubMed ID: 28946308
Publication DOI: 10.1016/j.foodchem.2017.07.154
Journal NLM ID: 7702639
Publisher: Elsevier Applied Science Publishers
Correspondence: Cheng HR <chenghr893

nenu.edu.cn>; Zhou YF <zhouyf383

nenu.edu.cn>
Institutions: Jilin Province Key Laboratory on Chemistry and Biology of Changbai Mountain Natural Drugs, School of Life Sciences, Northeast Normal University, Changchun, China

A novel polysaccharide AAMP-A70 (5.6 kDa) has been purified from the fruiting bodies of Amillariella mellea. Compositional analysis and 1H and 13C NMR spectra indicate that AAMP-A70 is a branched beta-glucan with a main chain that consists of beta-D-(1 -> 6) linked Glc rho residues substituted at O-3 by beta-Glc rho or alpha-D-(1 -> 6)-linked Gal rho side chains. AAMP-A70 increases macrophage phagocytosis and secretion of NO, ROS, TNF-alpha, IL-6 and IL-1 beta. Mechanistically, AAMP-A70 promotes degradation of I kappa B-alpha and nuclear translocation of the NF-kappa B p65 subunit, and enhances phosphorylation of MAPKs. In particular, the function blocking antibody to TLR2 substantially suppresses TNF-a and IL-6 production. Our data demonstrate that AAMP-A70 activates macrophages via NF-kappa B/MAPK signaling pathways and the TLR2 receptor. Overall, AAMP-A70 may serve as a good food supplement to enhance immunity.

polysaccharides, oxidative stress, Morchella conica

Structure type: structural motif or average structure ; 5600
Location inside paper: Abstract, fig. 2(A, B), AAMP-A70
Contained glycoepitopes: IEDB_135614,IEDB_136906,IEDB_137472,IEDB_141794,IEDB_141806,IEDB_142488,IEDB_146664,IEDB_151528,IEDB_190606,IEDB_241101,IEDB_983931,SB_192,SB_7

Methods: 13C NMR, 1H NMR, SDS-PAGE, ELISA, HPLC, GPC, extraction, statistical analysis, DEAE, methylation analysis, immunofluorescence analyses, determination of NO production, cell viability assay, precipitation, phenol-sulfuric acid assay, centrifugation, FT-IR, TFA hydrolysis, western blotting, citokine production, MTT assay
Biological activity: promotes macrophage activation, induces IκB-α degradation and NF-κB activation; activates MAPK pathways in RAW264.7

NCBI Taxonomy refs (TaxIDs): 47429
Reference(s) to other database(s): GTC:G79943VS
Show glycosyltransferases

NMR conditions: in D2O at 293 K

¹H NMR data: present in publication

¹³C NMR data: present in publication

There are 2 chemically distinct structures. Please, select:

[*]OC[C@H]1O[C@@H](OC[C@H]2O[C@@H](OC[C@H]3O[C@@H](OC[C@H]4O[C@@H]([*])[C@H](O)[C@@H](O)[C@@H]4O)[C@H](O)[C@@H](O)[C@@H]3O)[C@H](O)[C@@H](O)[C@@H]2O)[C@H](O)[C@@H](O[C@H]2O[C@H](CO[C@@H]3O[C@H](CO)[C@@H](O)[C@H](O)[C@H]3O)[C@H](O)[C@H](O)[C@H]2O)[C@@H]1O

SVG MW SMILES 3D mol Ionize