Taxonomic group: fungi / Ascomycota
(Phylum: Ascomycota)
Host organism: Nothofagus
Organ / tissue: fruiting body
The structure was elucidated in this paperPublication DOI: 10.1016/0008-6215(85)85109-0Journal NLM ID: 0043535Publisher: Elsevier
Institutions: Departamento de Química Orgánica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Buenos Aires, Argentina
A glycopeptide (In1) was isolated by phenol-water extraction from Cyttaria harioti Fischer, parasite of Nothofagus sps. Neutral sugars account for 89% of In1 and were characterized as glucose, mannose, and galactose. Glucosamine, identified by GLC, was colorimetrically estimated (5.8%). The molar ratio of Glc:Man:Gal:GlcNAc was 17:11:3:2. The linkages between the various monosaccharide residues were established through methylation analysis and periodate oxidation studies. The anomeric configurations of the various glycosyl groups were determined by chromium trioxide oxidation of the acetylated polysaccharide. The results were confirmed by 13C-NMR spectroscopy. The sugar chain is N-glycosyl-linked to the peptide. Structural features of the carbohydrate moiety of glycopeptide In1 are described
glycopeptide, Cyttaria harioti, carbohydrate moiety
Structure type: fragment of a bigger structure
Location inside paper: p. 262, structure 1
Aglycon: protein
Compound class: N-glucan
Contained glycoepitopes: IEDB_130701,IEDB_135813,IEDB_137340,IEDB_137485,IEDB_141793,IEDB_141807,IEDB_144983,IEDB_149158,IEDB_151531,IEDB_152206,IEDB_983930,SB_197,SB_198,SB_44,SB_67,SB_72,SB_73
Methods: gel filtration, 13C NMR, methylation, GLC-MS, acid hydrolysis, GLC, Smith degradation, paper chromatography, enzymatic digestion, extraction, periodate oxidation, optical rotation measurement, acetylation, acetolysis, chromium trioxide oxidation, reduction, column chromatography, dialysis, precipitation, phenol-sulfuric acid assay, derivatization, evaporation, Lowry method, phosphate measurement, Elson-Morgan method
NCBI Taxonomy refs (TaxIDs): 480491
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
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