Fukuda K, Hamada A Purification and chemical characterization of polysaccharides obtained from Lampteromyces japonicus by concanavalin A-sepharose affinity chromatography Biochimica et Biophysica Acta538(3) (1978)
580-592
/Variants 0/-a-D-Manp-(1-2)-a-D-Manp-(1-2)-+
|
-6)-a-D-Manp-(1-6)-a-D-Manp-(1-
/Variants 0/ is:
a-D-Manp-(1-2)-
OR (exclusively)
a-D-Galp-(1-2)-
The structure was elucidated in this paper NCBI PubMed ID:564212 Publication DOI:10.1016/0304-4165(78)90419-1 Journal NLM ID:0217513 Publisher: Elsevier Institutions: School of Pharmaceutical Sciences, Showa University, Shinagawa-ku, Japan
Two classes of neutral polysaccharide which could not be separated from each other by conventional methods were isolated from the fungus, Lampteromyces japonicus, by affinity chromatography using concanavalin A-Sepharose. The polysaccharide retained on the concanavalin A-Sepharose column was eluted with 0.05 M methyl α-D-mannopyranoside and appeared to be α-mannan, while that which passed through the column was virtually all β-glucan. Both polysaccharides were subjected to Smith-type degradation, methylation, acetolysis and glucosidase treatment. The results indicated that the α-mannan contained predominantly α-(1→2)-linked side chains branching from an α-(1→6)-linked backbone at the (1→2,6)-linked mannopyranosyl residues. Galactose was attached to approximately one-quarter of the non-reducing mannose terminals. The β-glucan seemed to contain mainly (1→6)-linked side chains branching from a (1→3)-linked backbone at the (1→3,6)-linked glucopyranosyl residues
Fukuda K, Hamada A Purification and chemical characterization of polysaccharides obtained from Lampteromyces japonicus by concanavalin A-sepharose affinity chromatography Biochimica et Biophysica Acta538(3) (1978)
580-592
The structure was elucidated in this paper NCBI PubMed ID:564212 Publication DOI:10.1016/0304-4165(78)90419-1 Journal NLM ID:0217513 Publisher: Elsevier Institutions: School of Pharmaceutical Sciences, Showa University, Shinagawa-ku, Japan
Two classes of neutral polysaccharide which could not be separated from each other by conventional methods were isolated from the fungus, Lampteromyces japonicus, by affinity chromatography using concanavalin A-Sepharose. The polysaccharide retained on the concanavalin A-Sepharose column was eluted with 0.05 M methyl α-D-mannopyranoside and appeared to be α-mannan, while that which passed through the column was virtually all β-glucan. Both polysaccharides were subjected to Smith-type degradation, methylation, acetolysis and glucosidase treatment. The results indicated that the α-mannan contained predominantly α-(1→2)-linked side chains branching from an α-(1→6)-linked backbone at the (1→2,6)-linked mannopyranosyl residues. Galactose was attached to approximately one-quarter of the non-reducing mannose terminals. The β-glucan seemed to contain mainly (1→6)-linked side chains branching from a (1→3)-linked backbone at the (1→3,6)-linked glucopyranosyl residues
Methods: methylation, IR, GC-MS, acid hydrolysis, GLC, Smith degradation, enzymatic digestion, extraction, affinity chromatography, periodate oxidation, optical rotation measurement, acetylation, acetolysis, methylation analysis, reduction, column chromatography, precipitation, phenol-sulfuric acid assay, Kirby method Enzymes that release or process the structure: β-glucosidase Comments, role: the published polymerization frame of a chemical repeating unit was shifted for compatibility with other records
Related record ID(s): 49235 NCBI Taxonomy refs (TaxIDs):72119 Reference(s) to other database(s): GTC:G92257RL Show glycosyltransferases
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(later renamed to: Omphalotus japonicus)