Taxonomic group: fungi / Basidiomycota
(Phylum: Basidiomycota)
Organ / tissue: fruiting body
The structure was elucidated in this paperNCBI PubMed ID: 18794771Publication DOI: 10.3390/molecules13081538Journal NLM ID: 100964009Publisher: Basel, Switzerland: MDPI
Correspondence: Yang WB <wbyang
gate.sinica.edu.tw>
Institutions: Genomics Research Center, Academia Sinica, No. 128, Academia Road Section 2, Nan-Kang, Taipei, Taiwan, Instrument Center, Taipei Medical University, Taipei, Taiwan
A novel method that uses matrix-assisted laser desorption/ionization (MALDI) mass spectrometry to analyze molecular weight and sequencing of glucan in Ganoderma lucidum is presented. Thus, β-glucan, which was isolated from fruiting bodies of G. lucidum, was measured in a direct and fast way using MALDI mass spectrometry. In addition, tandem mass spectrometry of permethylated glucans of G. lucidum, dextran, curdlan and maltohexaose were also pursued and different fragment patterns were obtained. The G. lucidum glucan structure was determined and this method for linkage analysis of permethylated glucan has been proven feasible.
polysaccharides, 2, β-glucan, matrix-assisted laser desorption/ionization (MALDI), Ganoderma lucidum, 5-dihydroxybenzoic acid (2, 5-DHB)
Structure type: structural motif or average structure ; n=4-16
Location inside paper: fig. 6
Compound class: glucan
Contained glycoepitopes: IEDB_1397514,IEDB_141806,IEDB_142488,IEDB_146664,IEDB_153543,IEDB_158555,IEDB_161166,IEDB_241101,IEDB_558869,IEDB_857743,IEDB_983931,SB_192
Methods: gel filtration, 13C NMR, GC-MS, HPAEC-PAD, acetylation, methylation analysis, reduction with NaBH4, MALDI-TOF MS/MS, HCl hydrolysis, TFA hydrolysis, 1 NMR
Related record ID(s): 42897, 42898
NCBI Taxonomy refs (TaxIDs): 5315Reference(s) to other database(s): GTC:G52845UF
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There is only one chemically distinct structure:
Found 
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(previously named: Boletus lucidus)