Taxonomic group: fungi / Basidiomycota
(Phylum: Basidiomycota)
Host organism: Betula pendula
Organ / tissue: fruiting body
NCBI PubMed ID: 31440877Publication DOI: 10.1007/s11033-019-05032-xJournal NLM ID: 0403234Publisher: Dordrecht, Boston, Reidel
Correspondence: Czerwonka A <arkadiusz.czerwonka
poczta.umcs.lublin.pl>; Wiater A <adrianw2
poczta.umcs.lublin.pl>; Komaniecka I <ikoma
hektor.umcs.lublin.pl>; Adamczyk P <paulinapolak2501
gmail.com>; Rzeski W <rzeskiw
hektor.umcs.lublin.pl>; Pleszczyńska M <m.pleszczynska
poczta.umcs.lublin.pl>
Institutions: Department of Industrial Microbiology, Maria Curie-Skłodowska University, Lublin, Poland, Department of Genetics and Microbiology, Maria Curie-Skłodowska University, Lublin, Poland, Department of Medical Biology, Institute of Rural Health, Lublin, Poland, Department of Virology and Immunology, Maria Curie-Skłodowska University, Lublin, Poland
Novel α-(1→3)-glucooligosaccharides (α-(1→3)-GOS) were prepared by acid hydrolysis of α-(1→3)-glucan isolated from Fomitopsis betulina fruiting bodies and characterized. Their anti-cancer potential was evaluated in in vitro assays in a colon cancer cell model. The tested α-(1→3)-GOS showed antiproliferative (MTT assay) and pro-apoptotic (Annexin V-FITC and PI technique) features against colon cancer but not against normal epithelial colon cells. Additionally, we did not observe cytotoxic activity (neutral red and lactate dehydrogenase assays) of α-(1→3)-GOS against several types of normal cell lines. In the present study, we demonstrated the anticancer potential of α-(1→3)-GOS in a colon carcinoma model. The anti-tumour effect of α-(1→3)-GOS is related with induction of apoptosis. Based on these results, we conclude that α-(1→3)-GOS may be considered as a dietary or therapeutic agent with an ability to inhibit the growth of cancer cells.
Colon cancer, α-(1→3)-glucan, Fomitopsis betulina, α-(1→3)-glucooligosaccharides
Structure type: homopolymer
Location inside paper: abstract
Trivial name: D-rhamnan, α-1,3-D-glucan, α-1,3-glucan, (1-3)-α-Glucan, 1-3-α-glucan, α-(1,3)-glucan, α-(1,3)glucan, (1-3)-α-glucan, pseudonigeran, α-(1,3)-glucan, pseudonigeran, water-insoluble α-D-glucan (TM-I)
Compound class: EPS, O-polysaccharide, cell wall polysaccharide, glucan, polysaccharide, D-glucan
Contained glycoepitopes: IEDB_142488,IEDB_144998,IEDB_146664,IEDB_983931,SB_192
Methods: acid hydrolysis, MALDI-TOF MS, biological assays, extraction, cell viability assay, evaporation, centrifugation, MTT, cells proliferation assay
Related record ID(s): 49982
NCBI Taxonomy refs (TaxIDs): 40450Reference(s) to other database(s): GTC:G02177KX, CCSD:
49417, CBank-STR:17683, GenDB:HM245773
Show glycosyltransferases
There is only one chemically distinct structure:
Taxonomic group: fungi / Basidiomycota
(Phylum: Basidiomycota)
Host organism: Betula pendula
Organ / tissue: fruiting body
NCBI PubMed ID: 31440877Publication DOI: 10.1007/s11033-019-05032-xJournal NLM ID: 0403234Publisher: Dordrecht, Boston, Reidel
Correspondence: Czerwonka A <arkadiusz.czerwonka
poczta.umcs.lublin.pl>; Wiater A <adrianw2
poczta.umcs.lublin.pl>; Komaniecka I <ikoma
hektor.umcs.lublin.pl>; Adamczyk P <paulinapolak2501
gmail.com>; Rzeski W <rzeskiw
hektor.umcs.lublin.pl>; Pleszczyńska M <m.pleszczynska
poczta.umcs.lublin.pl>
Institutions: Department of Industrial Microbiology, Maria Curie-Skłodowska University, Lublin, Poland, Department of Genetics and Microbiology, Maria Curie-Skłodowska University, Lublin, Poland, Department of Medical Biology, Institute of Rural Health, Lublin, Poland, Department of Virology and Immunology, Maria Curie-Skłodowska University, Lublin, Poland
Novel α-(1→3)-glucooligosaccharides (α-(1→3)-GOS) were prepared by acid hydrolysis of α-(1→3)-glucan isolated from Fomitopsis betulina fruiting bodies and characterized. Their anti-cancer potential was evaluated in in vitro assays in a colon cancer cell model. The tested α-(1→3)-GOS showed antiproliferative (MTT assay) and pro-apoptotic (Annexin V-FITC and PI technique) features against colon cancer but not against normal epithelial colon cells. Additionally, we did not observe cytotoxic activity (neutral red and lactate dehydrogenase assays) of α-(1→3)-GOS against several types of normal cell lines. In the present study, we demonstrated the anticancer potential of α-(1→3)-GOS in a colon carcinoma model. The anti-tumour effect of α-(1→3)-GOS is related with induction of apoptosis. Based on these results, we conclude that α-(1→3)-GOS may be considered as a dietary or therapeutic agent with an ability to inhibit the growth of cancer cells.
Colon cancer, α-(1→3)-glucan, Fomitopsis betulina, α-(1→3)-glucooligosaccharides
Structure type: oligomer
Location inside paper: abstract
Compound class: glucan, oligosaccharide
Contained glycoepitopes: IEDB_142488,IEDB_144998,IEDB_146664,IEDB_983931,SB_192
Methods: acid hydrolysis, MALDI-TOF MS, biological assays, extraction, cell viability assay, evaporation, centrifugation, MTT, cells proliferation assay
Biological activity: the IC50 values of α-(1 → 3)-GOS for the LS180, SW620, SW948, HT-29, and CCD 841 CoTr cell lines were estimated at 19.55, 14.92, 18.30, 17.06, and 39.38 mg/mL, respectively. α-(1→3)-GOS exerted an approximately two-fold stronger effect on the cancer than normal colon cells
Related record ID(s): 49983
NCBI Taxonomy refs (TaxIDs): 40450Reference(s) to other database(s): GTC:G63836BT
Show glycosyltransferases
There is only one chemically distinct structure:
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(previously named: Piptoporus betulinus)