Taxonomic group: fungi / Basidiomycota
(Phylum: Basidiomycota)
Organ / tissue: fruiting body
The structure was elucidated in this paperNCBI PubMed ID: 31173162Publication DOI: 10.3892/ijmm.2019.4219Journal NLM ID: 9810955Publisher: Athens, Greece: D.A. Spandidos
Correspondence: Hou Y <starthlh
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Institutions: Key Laboratory of Southwest China Wildlife Resources Conservation, College of Life Sciences, China West Normal University, Nanchong, China, College of Environmental Science and Engineering, China West Normal University, Nanchong, China
The present study investigated the structural characterization and immune regulation of a novel polysaccharide from Maerkang Lactarius deliciosus Gray. Chemical methods, high performance gel permeation chromatography, fourier transform infrared spectroscopy, nuclear magnetic resonance spectrum and gas chromatography-mass spectrometry were used to characterize the polysaccharide structure. The immunomodulatory abilities of the Maerkang L. deliciosus Gray polysaccharide (LDG-M) were also investigated. LDG-M was primarily composed of β-D-glucose and α-D-lyxose with the ratio of 2:1. The possible structure of LDG-M had a backbone of 1,6-linked-β-D-glucose and 1,4,6-linked-β-D-glucose, with branches primarily composed of one (1→4)-linked-α-D-lyxose residue. The immunoregulatory activity results demonstrated that LDG-M promoted the proliferation and phagocytosis of macrophages, and induced cytokine release. LDG-M also promoted the proliferation of B cells by affecting the G0/G1, S and G2/M phases. The present study introduced LDG-M as a valuable source with unique immunoregulatory properties.
polysaccharide, structure elucidation, immune regulation, Maerkang Lactarius deliciosus Gray
Structure type: structural motif or average structure ; 573689
Location inside paper: p. 718, Fig. 2, F, p. 716, Table I
Compound class: polysaccharide, lyxoglucan
Contained glycoepitopes: IEDB_135614,IEDB_141806,IEDB_142488,IEDB_146664,IEDB_241101,IEDB_983931,SB_192
Methods: 13C NMR, 1H NMR, methylation, IR, GC-MS, ELISA, acid hydrolysis, biological assays, extraction, acetylation, methylation analysis, reduction, column chromatography, fluorescence microscopy, dialysis, cytokine production, HPGPC, precipitation, macrophage activity assay, phenol-sulfuric acid assay, antitumor activity assay, centrifugation
Biological activity: polysaccharide could promote the proliferation and phagocytosis of macrophages, and induce cytokine (TNF-α and IL-6) release. It could also significantly promote the proliferative activity of B cells and promote B cells to enter S phase. Polysaccharide significantly enhanced the inhibitory activity of RAW264.7 cells on L929 cells, which further indicated that polysaccharide exhibited immunological activities
Comments, role: NMR temperature was not specified; -6)Glc(1- С6 signal assignment seems erroneous; published erroneous NMR chemical shift of #4_aDLyxp С5 (72.78) was removed by CSDB staff
NCBI Taxonomy refs (TaxIDs): 55514Reference(s) to other database(s): GTC:G95101XO
Show glycosyltransferases
NMR conditions: in D2O
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6
6 bDGlcp 102.67 71.71 79.50 71.27 72.50 71.11
4 aDLyxp 100.79 71.78 72.19 71.15 ?
bDGlcp 102.69 71.29 70.88 72.06 73.00 69.35
1H NMR data: present in publication
|
13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 |
|---|
| 6 | bDGlcp | 102.67 | 71.71 | 79.50 | 71.27 | 72.50 | 71.11 |
| 4 | aDLyxp | 100.79 | 71.78 | 72.19 | 71.15 | ? | |
| | bDGlcp | 102.69 | 71.29 | 70.88 | 72.06 | 73.00 | 69.35 |
|
The spectrum also has 1 signal at unknown position (not plotted). |
There is only one chemically distinct structure:
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