Taxonomic group: fungi / Ascomycota
(Phylum: Ascomycota)
The structure was elucidated in this paperNCBI PubMed ID: 31634426Publication DOI: 10.1021/acs.jafc.9b05340Journal NLM ID: 0374755Publisher: American Chemical Society
Correspondence: Nie S <spnie
ncu.edu.cn>
Institutions: School of Medicine, University of Cardiff, Cardiff, UK, Phillips Hydrocolloids Research Centre, Glyndwr University, Wrexham, UK, Guelph Research and Development Centre, Agriculture and Agri-Food Canada, Guelph, Canada, State Key Laboratory of Food Science and Technology, China-Canada Joint Lab of Food Science and Technology (Nanchang), Nanchang University, Nanchang, China
Water-soluble β-D-glucan was obtained from wild Cordyceps sinensis by alkali solution and ethanol precipitation. The structure characteristics were determined using high-performance anion-exchange chromatography coupled with pulsed amperometric detection (HPAEC-PAD), methylation combined with gas chromatography-mass spectrometry, and one-/two-dimensional nuclear magnetic resonance spectroscopy. Results showed that β-D-glucan had a structure of every seven (1→3)-β-D-Glcp backbone residues with two (1→6)-β-D-Glcp branches. Additionally, conformation properties in different solvents were investigated by static light scattering, dynamic light scattering, and HPSEC with multiple detectors. It was found that β-D-glucan in 0.5 M NaOH had a narrow unimodal distribution of hydrodynamic radius displaying a spherical coil conformation, whereas it formed severe aggregation in dimethyl sulfoxide. In 0.1 M NaNO3, β-D-glucan mainly existed as a rod-like conformation corresponding to a helical structure together with small aggregates (10%). This work added more information to the understanding of C. sinensis polysaccharides.
conformation, structure, β-D-glucan, Cordyceps sinensis
Structure type: structural motif or average structure ; 477000
Location inside paper: p. 12524, Fig. 2, p. 12524, Table 2
Compound class: glucan, polysaccharide
Contained glycoepitopes: IEDB_1397514,IEDB_141806,IEDB_142488,IEDB_146664,IEDB_153543,IEDB_158555,IEDB_161166,IEDB_241101,IEDB_558869,IEDB_857743,IEDB_983931,SB_192
Methods: 13C NMR, 1H NMR, NMR-2D, methylation, GC-MS, acid hydrolysis, HPSEC, enzymatic digestion, extraction, acetylation, methylation analysis, reduction, dialysis, dynamic light scattering, precipitation, static light scattering, HPAEC−PAD
NCBI Taxonomy refs (TaxIDs): 72228Reference(s) to other database(s): GTC:G10489HD
Show glycosyltransferases
NMR conditions: in D2O at 313 K
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6
3,3,3,3,3,3 bDGlcp 102.72 72.36 86.03 68.17 75.92 60.59
3,3,3,3,3 bDGlcp 102.72 72.36 86.03 68.17 75.92 60.59
3,3,3,3,6 bDGlcp 102.72 73.38 75.93 69.73 76.34 60.59
3,3,3,3 bDGlcp 102.72 72.36 86.03 68.17 74.34 68.09
3,3,3 bDGlcp 102.72 72.36 86.03 68.17 75.92 60.59
3,3,6 bDGlcp 102.72 73.38 75.93 69.73 76.34 60.59
3,3 bDGlcp 102.72 72.36 86.03 68.17 74.34 68.09
3 bDGlcp 102.72 72.36 86.03 68.17 75.92 60.59
bDGlcp 102.72 72.36 86.03 68.17 75.92 60.59
1H NMR data:
Linkage Residue H1 H2 H3 H4 H5 H6
3,3,3,3,3,3 bDGlcp 4.52 3.27-3.29 3.48 3.24 3.27 3.44-3.69
3,3,3,3,3 bDGlcp 4.52 3.27-3.29 3.48 3.24 3.27 3.44-3.69
3,3,3,3,6 bDGlcp 4.22 2.99 3.16 3.06 3.11 3.44-3.68
3,3,3,3 bDGlcp 4.52 3.32 3.48 3.25 3.52 3.52-4.08
3,3,3 bDGlcp 4.52 3.27-3.29 3.48 3.24 3.27 3.44-3.69
3,3,6 bDGlcp 4.22 2.99 3.16 3.06 3.11 3.44-3.68
3,3 bDGlcp 4.52 3.32 3.48 3.25 3.52 3.52-4.08
3 bDGlcp 4.52 3.27-3.29 3.48 3.24 3.27 3.44-3.69
bDGlcp 4.52 3.27-3.29 3.48 3.24 3.27 3.44-3.69
1H/13C HSQC data:
Linkage Residue C1/H1 C2/H2 C3/H3 C4/H4 C5/H5 C6/H6
3,3,3,3,3,3 bDGlcp 102.72/4.52 72.36/3.27-3.29 86.03/3.48 68.17/3.24 75.92/3.27 60.59/3.44-3.69
3,3,3,3,3 bDGlcp 102.72/4.52 72.36/3.27-3.29 86.03/3.48 68.17/3.24 75.92/3.27 60.59/3.44-3.69
3,3,3,3,6 bDGlcp 102.72/4.22 73.38/2.99 75.93/3.16 69.73/3.06 76.34/3.11 60.59/3.44-3.68
3,3,3,3 bDGlcp 102.72/4.52 72.36/3.32 86.03/3.48 68.17/3.25 74.34/3.52 68.09/3.52-4.08
3,3,3 bDGlcp 102.72/4.52 72.36/3.27-3.29 86.03/3.48 68.17/3.24 75.92/3.27 60.59/3.44-3.69
3,3,6 bDGlcp 102.72/4.22 73.38/2.99 75.93/3.16 69.73/3.06 76.34/3.11 60.59/3.44-3.68
3,3 bDGlcp 102.72/4.52 72.36/3.32 86.03/3.48 68.17/3.25 74.34/3.52 68.09/3.52-4.08
3 bDGlcp 102.72/4.52 72.36/3.27-3.29 86.03/3.48 68.17/3.24 75.92/3.27 60.59/3.44-3.69
bDGlcp 102.72/4.52 72.36/3.27-3.29 86.03/3.48 68.17/3.24 75.92/3.27 60.59/3.44-3.69
1H NMR data:
| Linkage | Residue | H1 | H2 | H3 | H4 | H5 | H6 |
|---|
| 3,3,3,3,3,3 | bDGlcp | 4.52 | 3.27
3.29 | 3.48 | 3.24 | 3.27 | 3.44
3.69 |
| 3,3,3,3,3 | bDGlcp | 4.52 | 3.27
3.29 | 3.48 | 3.24 | 3.27 | 3.44
3.69 |
| 3,3,3,3,6 | bDGlcp | 4.22 | 2.99 | 3.16 | 3.06 | 3.11 | 3.44
3.68 |
| 3,3,3,3 | bDGlcp | 4.52 | 3.32 | 3.48 | 3.25 | 3.52 | 3.52
4.08 |
| 3,3,3 | bDGlcp | 4.52 | 3.27
3.29 | 3.48 | 3.24 | 3.27 | 3.44
3.69 |
| 3,3,6 | bDGlcp | 4.22 | 2.99 | 3.16 | 3.06 | 3.11 | 3.44
3.68 |
| 3,3 | bDGlcp | 4.52 | 3.32 | 3.48 | 3.25 | 3.52 | 3.52
4.08 |
| 3 | bDGlcp | 4.52 | 3.27
3.29 | 3.48 | 3.24 | 3.27 | 3.44
3.69 |
| | bDGlcp | 4.52 | 3.27
3.29 | 3.48 | 3.24 | 3.27 | 3.44
3.69 |
|
13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 |
|---|
| 3,3,3,3,3,3 | bDGlcp | 102.72 | 72.36 | 86.03 | 68.17 | 75.92 | 60.59 |
| 3,3,3,3,3 | bDGlcp | 102.72 | 72.36 | 86.03 | 68.17 | 75.92 | 60.59 |
| 3,3,3,3,6 | bDGlcp | 102.72 | 73.38 | 75.93 | 69.73 | 76.34 | 60.59 |
| 3,3,3,3 | bDGlcp | 102.72 | 72.36 | 86.03 | 68.17 | 74.34 | 68.09 |
| 3,3,3 | bDGlcp | 102.72 | 72.36 | 86.03 | 68.17 | 75.92 | 60.59 |
| 3,3,6 | bDGlcp | 102.72 | 73.38 | 75.93 | 69.73 | 76.34 | 60.59 |
| 3,3 | bDGlcp | 102.72 | 72.36 | 86.03 | 68.17 | 74.34 | 68.09 |
| 3 | bDGlcp | 102.72 | 72.36 | 86.03 | 68.17 | 75.92 | 60.59 |
| | bDGlcp | 102.72 | 72.36 | 86.03 | 68.17 | 75.92 | 60.59 |
|
There is only one chemically distinct structure:
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Found 1 record.
Displayed record 1
(later renamed to: Ophiocordyceps sinensis)