Found 3 records.
Displayed records from 1 to 3
Expand all records
Collapse all records
Show all as text (SweetDB notation)
Show all graphically (SNFG notation)
Singh RS, Singh T, Pandey A
Fungal endoinulinase production from raw Asparagus inulin for the production of fructooligosaccharides
Bioresource Technology Reports 10 (2020)
ID 100417
Aspergillus tritici BGPUP6
(Ancestor NCBI TaxID 478866,
species name lookup)
Taxonomic group: fungi / Ascomycota
(Phylum: Ascomycota)
Publication DOI: 10.1016/j.biteb.2020.100417Journal NLM ID: 101747079Publisher: Amsterdam: Elsevier Ltd.
Correspondence: Singh RS <rssbt

pbi.ac.in>
Institutions: Carbohydrate and Protein Biotechnology Laboratory, Department of Biotechnology, Punjabi University, Patiala, India, Centre for Innovation and Translational Research, CSIR Indian Institute of Toxicology, Lucknow, India
In present study, statistical optimization of medium composition for endoinulinase production from raw inulin using Aspergillus tritici was performed in shake-flask fermentations. The concentration of four independent variables viz. raw inulin (1.5–3.5%), peptone (0.2–1.8%), (NH4)H2PO4 (0.2–1.8%) and pH (4.5–6.5) was explored to optimize the composition of fermentation medium for maximum endoinulinase production. Fermentation medium containing 2.5% raw inulin, 1% peptone, 1% (NH4)H2PO4 and medium pH adjusted to 5.5 supported maximum (25.39 IU/mL) endoinulinase production. Adequate precision value >4, closeness of coefficient of determination (R2) value to 1, good co-relation between the predicted and experimental values, percentage error <5%, high F-value 164.52 and low Lack of fit F-value 0.19 authenticates the fitness of quadratic model. TLC fingerprints studies confirmed fructooligosaccharides synthesis from inulin by crude fungal endoinulinase. Fructooligosaccharides yield (19.40%) containing 3.70% ketose (GF2), 2.71% nystose (GF3) and 1.42% fructofuranosyl nystose (GF4) were obtained from inulin (10%).
response surface methodology, fructooligosaccharides, endoinulinase, Aspergillus tritici, raw-inulin
Structure type: oligomer
Location inside paper: Fig. 4, GF2
Trivial name: 1-kestose, inulin
Compound class: fructan, trisaccharide, oligosaccharide, fructo-oligosaccharide
Contained glycoepitopes: IEDB_142488,IEDB_144998,IEDB_146664,IEDB_983931,SB_192
Methods: TLC, enzymatic digestion, extraction, cell growth, enzymatic assay, anthrone-sulfuric acid assay, DNS method, total sugars measurement, reducing sugar measurement
Enzymes that release or process the structure: endoinulinase
Synthetic data: enzymatic
Related record ID(s): 5201, 43612, 44206, 44306, 44492, 45817, 49006, 49009, 49505, 49521, 49525, 49545, 49557, 49565, 49569, 49571, 49574, 50321, 50325, 50329, 50332, 50342, 50345, 50348, 50351, 50355, 50359, 50360, 50361, 147005, 213017, 219959, 245753, 246867
NCBI Taxonomy refs (TaxIDs): 478866Reference(s) to other database(s): GTC:G11260CN, GlycomeDB:
396, CCSD:
26122, CBank-STR:5624, GenDB:MH445969, US-PT:US2017298332A1
Show glycosyltransferases
There is only one chemically distinct structure:
Expand this record
Collapse this record
Singh RS, Singh T, Pandey A
Fungal endoinulinase production from raw Asparagus inulin for the production of fructooligosaccharides
Bioresource Technology Reports 10 (2020)
ID 100417
Aspergillus tritici BGPUP6
(Ancestor NCBI TaxID 478866,
species name lookup)
Taxonomic group: fungi / Ascomycota
(Phylum: Ascomycota)
Publication DOI: 10.1016/j.biteb.2020.100417Journal NLM ID: 101747079Publisher: Amsterdam: Elsevier Ltd.
Correspondence: Singh RS <rssbt

pbi.ac.in>
Institutions: Carbohydrate and Protein Biotechnology Laboratory, Department of Biotechnology, Punjabi University, Patiala, India, Centre for Innovation and Translational Research, CSIR Indian Institute of Toxicology, Lucknow, India
In present study, statistical optimization of medium composition for endoinulinase production from raw inulin using Aspergillus tritici was performed in shake-flask fermentations. The concentration of four independent variables viz. raw inulin (1.5–3.5%), peptone (0.2–1.8%), (NH4)H2PO4 (0.2–1.8%) and pH (4.5–6.5) was explored to optimize the composition of fermentation medium for maximum endoinulinase production. Fermentation medium containing 2.5% raw inulin, 1% peptone, 1% (NH4)H2PO4 and medium pH adjusted to 5.5 supported maximum (25.39 IU/mL) endoinulinase production. Adequate precision value >4, closeness of coefficient of determination (R2) value to 1, good co-relation between the predicted and experimental values, percentage error <5%, high F-value 164.52 and low Lack of fit F-value 0.19 authenticates the fitness of quadratic model. TLC fingerprints studies confirmed fructooligosaccharides synthesis from inulin by crude fungal endoinulinase. Fructooligosaccharides yield (19.40%) containing 3.70% ketose (GF2), 2.71% nystose (GF3) and 1.42% fructofuranosyl nystose (GF4) were obtained from inulin (10%).
response surface methodology, fructooligosaccharides, endoinulinase, Aspergillus tritici, raw-inulin
Structure type: oligomer
Location inside paper: Fig. 4, GF3
Trivial name: nystose, inulin, 1,1-kestotetraose
Compound class: fructan, tetrasaccharide, oligosaccharide
Contained glycoepitopes: IEDB_142488,IEDB_144998,IEDB_146664,IEDB_923067,IEDB_983931,SB_192
Methods: TLC, enzymatic digestion, extraction, cell growth, enzymatic assay, anthrone-sulfuric acid assay, DNS method, total sugars measurement, reducing sugar measurement
Enzymes that release or process the structure: endoinulinase
Synthetic data: enzymatic
Related record ID(s): 5421, 43613, 44207, 44307, 44493, 49506, 49522, 49526, 49546, 49566, 49572, 49575, 50322, 50326, 50330, 50333, 50343, 50346, 50349, 50352, 50356, 50358, 50360, 50362, 100071, 147006, 244675, 269959
NCBI Taxonomy refs (TaxIDs): 478866Reference(s) to other database(s): GTC:G60726JL, GlycomeDB:
63, CCSD:
23519, CBank-STR:9595, GenDB:MH445969, US-PT:US2017298332A1
Show glycosyltransferases
There is only one chemically distinct structure:
Expand this record
Collapse this record
Singh RS, Singh T, Pandey A
Fungal endoinulinase production from raw Asparagus inulin for the production of fructooligosaccharides
Bioresource Technology Reports 10 (2020)
ID 100417
Aspergillus tritici BGPUP6
(Ancestor NCBI TaxID 478866,
species name lookup)
Taxonomic group: fungi / Ascomycota
(Phylum: Ascomycota)
Publication DOI: 10.1016/j.biteb.2020.100417Journal NLM ID: 101747079Publisher: Amsterdam: Elsevier Ltd.
Correspondence: Singh RS <rssbt

pbi.ac.in>
Institutions: Carbohydrate and Protein Biotechnology Laboratory, Department of Biotechnology, Punjabi University, Patiala, India, Centre for Innovation and Translational Research, CSIR Indian Institute of Toxicology, Lucknow, India
In present study, statistical optimization of medium composition for endoinulinase production from raw inulin using Aspergillus tritici was performed in shake-flask fermentations. The concentration of four independent variables viz. raw inulin (1.5–3.5%), peptone (0.2–1.8%), (NH4)H2PO4 (0.2–1.8%) and pH (4.5–6.5) was explored to optimize the composition of fermentation medium for maximum endoinulinase production. Fermentation medium containing 2.5% raw inulin, 1% peptone, 1% (NH4)H2PO4 and medium pH adjusted to 5.5 supported maximum (25.39 IU/mL) endoinulinase production. Adequate precision value >4, closeness of coefficient of determination (R2) value to 1, good co-relation between the predicted and experimental values, percentage error <5%, high F-value 164.52 and low Lack of fit F-value 0.19 authenticates the fitness of quadratic model. TLC fingerprints studies confirmed fructooligosaccharides synthesis from inulin by crude fungal endoinulinase. Fructooligosaccharides yield (19.40%) containing 3.70% ketose (GF2), 2.71% nystose (GF3) and 1.42% fructofuranosyl nystose (GF4) were obtained from inulin (10%).
response surface methodology, fructooligosaccharides, endoinulinase, Aspergillus tritici, raw-inulin
Structure type: oligomer
Location inside paper: Fig. 4, GF4
Trivial name: 1-fructofuranosylsylnystose, 1-fructofuranosylnystose
Compound class: pentasaccharide, oligosaccharide
Contained glycoepitopes: IEDB_142488,IEDB_144998,IEDB_146664,IEDB_923067,IEDB_983931,SB_192
Methods: TLC, enzymatic digestion, extraction, cell growth, enzymatic assay, anthrone-sulfuric acid assay, DNS method, total sugars measurement, reducing sugar measurement
Enzymes that release or process the structure: endoinulinase
Synthetic data: enzymatic
Related record ID(s): 44208, 44308, 49507, 49527, 49547, 49573, 49576, 50323, 50331, 50344, 50347, 50350, 50353, 50357, 50358, 50359, 50363, 269960
NCBI Taxonomy refs (TaxIDs): 478866Reference(s) to other database(s): GenDB:MH445969, US-PT:US2017298332A1
Show glycosyltransferases
There is only one chemically distinct structure:
Expand this record
Collapse this record
Total list of record IDs on all result pages of the current query:
Execution: 7 sec