Taxonomic group: fungi / Mucoromycota
(Phylum: Mucoromycota)
NCBI PubMed ID: 32829843Publication DOI: 10.1016/j.carbpol.2020.116716Journal NLM ID: 8307156Publisher: Elsevier
Correspondence: jccarvalho
ufpr.br
Institutions: Department of Biochemistry and Molecular Biology, Federal University of Paraná, Curitiba, Brazil, Federal University of Paraná, Department of Bioprocess Engineering and Biotechnology, Curitiba, Brazil, Faculdades Pequeno Príncipe, Curitiba, Brazil, BioPol, Chemistry Department, Federal University of Paraná, Centro Politécnico, Curitiba, Brazil
The production of a chitin-like exopolysaccharide (EPS) was optimized through experimental design methods, evaluating the influence of urea, phosphate, and glucose. Under optimized conditions, up to 1.51 g/L was produced and its physicochemical characteristics were evaluated by chromatography, NMR, and FTIR spectroscopy, and rheological techniques. The results showed a homogeneous EPS (Mw 490000 g/mol) composed of chitin, linear polymer of β-(1→4)-linked N-acetyl-D-glucosamine residues. The acetylation degree as determined by 13C CP-MAS NMR spectroscopy was over 90 %. The EPS biological activities, such as antioxidant effect and antitumor properties, were evaluated. To the best of our knowledge, this is the first study on the production of a new alternative of extracellular chitin-like polysaccharide with promising bioactive properties from the filamentous fungus M. alpina.
exopolysaccharide, chemical characterization, chitin, Mortierella alpina, antitumoral
Structure type: homopolymer ; 490000
Location inside paper: Fig. 3
Trivial name: chitin
Compound class: EPS, polysaccharide
Contained glycoepitopes: IEDB_135813,IEDB_137340,IEDB_141807,IEDB_151531,IEDB_153212,IEDB_241099,IEDB_423114,IEDB_423150,SB_74,SB_85
Methods: deacetylation, 13C NMR, 1H NMR, NMR-2D, IR, inhibition studies, acid hydrolysis, HPSEC, viscosity measurement, acetylation, reduction, cell growth, dialysis, antioxidant activities, cytotoxicity assay, rheological study, spectrophotometry, Bradford method, anthrone-sulfuric acid assay, MTT, filtration, 13C CPMAS NMR, GC-MS/MS
Biological activity: polysaccharide had significant tumoral cell growth inhibition of >50% at 1.37 mg/mL for H295R, 2.1 mg/mL for CACO-2, 1.5 mg/mL for MDA MB 231, 1.4 mg/mL for MDA MB 468 and 1.3 mg/mL for MCF 07
NCBI Taxonomy refs (TaxIDs): 64518Reference(s) to other database(s): GTC:G97099AY
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
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