Taxonomic group: fungi / Basidiomycota, Basidiomycota, Basidiomycota, Basidiomycota, Basidiomycota, Ascomycota, Ascomycota, Ascomycota, Chytridiomycota (Phylum: Basidiomycota, Basidiomycota, Basidiomycota, Basidiomycota, Basidiomycota, Ascomycota, Ascomycota, Ascomycota, Chytridiomycota)
Associated disease: infection due to Aspergillus flavus [ICD11: XN6B8 ]; infection due to Aspergillus fumigatus [ICD11: XN5Z7 ]
 
NCBI PubMed ID: 32753587
Publication DOI: 10.1038/s41467-020-17615-5
Journal NLM ID: 101528555
Publisher: London: Nature Publishing Group
Correspondence: <becardlrsv.ups-tlse.fr>; <jeanmichel.anewisc.edu>
Institutions: Department of Medical Microbiology and Immunology, University of Wisconsin-Madison, Madison, USA, Laboratoire de Recherche en Sciences Végétales, Université de Toulouse, CNRS, UPS, Castanet-Tolosan, France, Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, USA, Department of Bacteriology, University of Wisconsin-Madison, Madison, USA, Department of Agronomy, University of Wisconsin-Madison, Madison, USA, Bioscience Division, Oak Ridge National Laboratory, Oak Ridge, USA, Laboratoire Évolution et Diversité Biologique, Université de Toulouse, CNRS, UPS, IRD, Toulouse, France, Laboratoire des Interactions Plantes-Microorganismes, Université de Toulouse, INRAE, CNRS, Castanet-Tolosan, France, South Dakota State University, Brookings, USA, North Carolina State University, Raleigh, USA, Department of Medicine, University of Wisconsin-Madison, Madison, USA, Department of Microbiology, University of Tennessee, Knoxville, USA, University of Georgia, Athens, USA, Department of Chemical Engineering, University of California, Santa Barbara, USA, Univ. Grenoble Alpes, CNRS, CERMAV, Grenoble, France, Laboratoire des Interactions Moléculaires et Réactivités Chimiques et Photochimiques, Université de Toulouse, CNRS, UPS, Toulouse, France, Department of Biochemistry, University of Wisconsin-Madison, Madison, USA

Lipo-chitooligosaccharides (LCOs) are signaling molecules produced by rhizobial bacteria that trigger the nodulation process in legumes, and by some fungi that also establish symbiotic relationships with plants, notably the arbuscular and ecto mycorrhizal fungi. Here, we show that many other fungi also produce LCOs. We tested 59 species representing most fungal phyla, and found that 53 species produce LCOs that can be detected by functional assays and/or by mass spectroscopy. LCO treatment affects spore germination, branching of hyphae, pseudohyphal growth, and transcription in non-symbiotic fungi from the Ascomycete and Basidiomycete phyla. Our findings suggest that LCO production is common among fungi, and LCOs may function as signals regulating fungal growth and development.

symbiosis, lipo-chitooligosaccharides, regulatory signals, fungal growth

Structure type: oligomer
Location inside paper: Fig. 2, a, b
Compound class: lipochitooligosaccharide
Contained glycoepitopes: IEDB_115015,IEDB_135813,IEDB_137340,IEDB_141181,IEDB_141807,IEDB_142489,IEDB_149135,IEDB_151531,IEDB_153212,IEDB_241099,IEDB_241120,SB_74,SB_85,SB_86
 
Methods: PCR, extraction, LC-MS, cell growth, RNA sequencing, gene expression, RNA extraction, evaporation, HILIC, filtration, HPLC-QTRAP-ESI-MS/MS
 
Related record ID(s): 51164
NCBI Taxonomy refs (TaxIDs): 1842050, 1132860, 71150, 29883, 76867, 5022, 5059, 746128, 1123529
Show glycosyltransferases
 

Convert structure to SMILES & 3D GlycoCT β-test WURCS 2.0 GLYCAM GlycoCT (external) GlycoCT XML (ext) GlydeII XML LinUCS Fragmentize Mol. weight

Simulate NMR spectra (GODDESS)

Show Sweet-II 3D model Generate 3D coords by GLYCAM (Sweet-II / GLYCAM will utilize a structure with sub-repeating units replaced by their multiple instances)

Taxonomic group: fungi / Mucoromycota (Phylum: Mucoromycota)
 
NCBI PubMed ID: 32753587
Publication DOI: 10.1038/s41467-020-17615-5
Journal NLM ID: 101528555
Publisher: London: Nature Publishing Group
Correspondence: <becardlrsv.ups-tlse.fr>; <jeanmichel.anewisc.edu>
Institutions: Department of Medical Microbiology and Immunology, University of Wisconsin-Madison, Madison, USA, Laboratoire de Recherche en Sciences Végétales, Université de Toulouse, CNRS, UPS, Castanet-Tolosan, France, Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, USA, Department of Bacteriology, University of Wisconsin-Madison, Madison, USA, Department of Agronomy, University of Wisconsin-Madison, Madison, USA, Bioscience Division, Oak Ridge National Laboratory, Oak Ridge, USA, Laboratoire Évolution et Diversité Biologique, Université de Toulouse, CNRS, UPS, IRD, Toulouse, France, Laboratoire des Interactions Plantes-Microorganismes, Université de Toulouse, INRAE, CNRS, Castanet-Tolosan, France, South Dakota State University, Brookings, USA, North Carolina State University, Raleigh, USA, Department of Medicine, University of Wisconsin-Madison, Madison, USA, Department of Microbiology, University of Tennessee, Knoxville, USA, University of Georgia, Athens, USA, Department of Chemical Engineering, University of California, Santa Barbara, USA, Univ. Grenoble Alpes, CNRS, CERMAV, Grenoble, France, Laboratoire des Interactions Moléculaires et Réactivités Chimiques et Photochimiques, Université de Toulouse, CNRS, UPS, Toulouse, France, Department of Biochemistry, University of Wisconsin-Madison, Madison, USA

Lipo-chitooligosaccharides (LCOs) are signaling molecules produced by rhizobial bacteria that trigger the nodulation process in legumes, and by some fungi that also establish symbiotic relationships with plants, notably the arbuscular and ecto mycorrhizal fungi. Here, we show that many other fungi also produce LCOs. We tested 59 species representing most fungal phyla, and found that 53 species produce LCOs that can be detected by functional assays and/or by mass spectroscopy. LCO treatment affects spore germination, branching of hyphae, pseudohyphal growth, and transcription in non-symbiotic fungi from the Ascomycete and Basidiomycete phyla. Our findings suggest that LCO production is common among fungi, and LCOs may function as signals regulating fungal growth and development.

symbiosis, lipo-chitooligosaccharides, regulatory signals, fungal growth

Structure type: oligomer
Location inside paper: Fig. 2, a, b
Compound class: lipochitooligosaccharide
Contained glycoepitopes: IEDB_115015,IEDB_135813,IEDB_137340,IEDB_141181,IEDB_141807,IEDB_142489,IEDB_149135,IEDB_151531,IEDB_153212,IEDB_241099,IEDB_241120,SB_74,SB_85,SB_86
 
Methods: PCR, extraction, LC-MS, cell growth, RNA sequencing, gene expression, RNA extraction, evaporation, HILIC, filtration, HPLC-QTRAP-ESI-MS/MS
 
Related record ID(s): 50880
NCBI Taxonomy refs (TaxIDs): 588596, 4876, 94130, 44941
Show glycosyltransferases
 

Convert structure to SMILES & 3D GlycoCT β-test WURCS 2.0 GLYCAM GlycoCT (external) GlycoCT XML (ext) GlydeII XML LinUCS Fragmentize Mol. weight

Simulate NMR spectra (GODDESS)

Show Sweet-II 3D model Generate 3D coords by GLYCAM (Sweet-II / GLYCAM will utilize a structure with sub-repeating units replaced by their multiple instances)