Taxonomic group: fungi / Basidiomycota
(Phylum: Basidiomycota)
Organ / tissue: fruiting bodies,
mushroom powder
The structure was elucidated in this paperPublication DOI: 10.1016/j.foodhyd.2021.107392Journal NLM ID: 8701770Publisher: New York, NY: Elsevier
Correspondence: M. Iacomini <iacomini
ufpr.br>
Institutions: Department of Biochemistry and Molecular Biology, Federal University of Parana, CEP 81531-980, Curitiba-PR, Brazil, Departament of Cell Biology, Federal University of Paraná, Curitiba, PR, Brazil, Faculdades Pequeno Príncipe, CEP 80230-020, Curitiba, PR, Brazil, Department of Biosciences, Federal University of Paraná, CEP 85950-000, Palotina-PR, Brazil
Regardless of the wealth of knowledge about the medicinal properties and industrial applications of fungal compounds, polysaccharides of some species of mushrooms remain unstudied. Therefore, fruiting bodies of the giant M. titans mushroom were chosen to extract polysaccharides and evaluate their physicochemical properties. Alkaline extraction yielded GFI fraction, which was composed of β-D-glucans and α-D-glucans (glycogen). This fraction underwent amylase treatment giving rise to a fraction that left only β-D-glucans of different molar masses (β-GLC) that were composed of (1→3)-linked β-D-Glcp units substituted at O-6 by (1→6)-linked β-D-Glcp or single units of β-D-Glcp. Both GFI (at 2% w/w) and β-GLC (at 1.5% w/w), were analyzed about their rheological properties and presented a non-Newtonian shear-thinning behavior and gel-like behavior under low temperature. It was confirmed that the gel-like behavior of GFI was due to the presence of β-glucans, once the fraction with isolated β-glucans (β-GLC) produced a stronger gel than the mixture with glycogen. This is probably due to a better association and network formation among β-glucans. Furthermore, methylated analyses showed a great amount of (1→6)-linked Glcp units, which was observed to produce a weaker gel in comparison to other glucans. This study presented for the first time that glucans extracted from M. titans may form gel under suitable conditions, enabling their industrial application as thickening or gelling agents.
polysaccharide, Rheology, gel, β-Glucans, Macrocybe titans
Structure type: fragment of a bigger structure
Location inside paper: abtract, p. 107392-4, β-GLC
Compound class: β-glucan
Contained glycoepitopes: IEDB_1397514,IEDB_141806,IEDB_142488,IEDB_146664,IEDB_153543,IEDB_158555,IEDB_161166,IEDB_241101,IEDB_558869,IEDB_857743,IEDB_983931,SB_192
Methods: 13C NMR, 1H NMR, NMR-2D, methylation, GC-MS, Smith degradation, composition analysis, HPSEC, extraction, acetylation, statistical analysis, partial hydrolysis, rheological study, Bradford method, DEPT-135, Congo red assay, α-amylase treatment
Comments, role: GFI fraction was treatment with α-amylase
NCBI Taxonomy refs (TaxIDs): 56473
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
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