Taxonomic group: fungi / Ascomycota
(Phylum: Ascomycota)
NCBI PubMed ID: 35229356Publication DOI: 10.1002/cbf.3690Journal NLM ID: 8305874Publisher: Oxford, England: Wiley-Blackwell
Correspondence: E.A.I.F. de Queiroz <eveline.queiroz
ufmt.br>; <eveline.ufmt
gmail.com>
Institutions: Núcleo de Pesquisa e Apoio Didático em Saúde (NUPADS), Programa de Pós-Graduação Ciências em Saúde, Instituto de Ciências da Saúde, Universidade Federal de Mato Grosso, Câmpus Universitário de Sinop, Sinop, Brazil, Instituto de Ciências da Saúde, Universidade Federal de Mato Grosso, Câmpus Universitário de Sinop, Sinop, Brazil, Beta-Glucan Produtos Farmoquímicos EIRELI, Lote 24A, Bloco Zircônia, Universidade Tecnológica Federal do Paraná, Câmpus Londrina, Londrina, Brazil, Programa de Pós-Graduação em Ciências Ambientais, Instituto de Ciências Naturais, Humanas e Sociais, Universidade Federal de Mato Grosso, Câmpus Universitário de Sinop, Sinop, Brazil
Botryosphaeran, a (1→3)(1→6)-β-D-glucan, presents several beneficial activities, such as antiproliferative, hypoglycemic and antitumoural activities. This study evaluated the effects of botryosphaeran on oxidative stress, inflammation and metabolic activities in Walker-256 tumour-bearing non-obese and obese rats. Wistars rats were divided into four groups: control tumour (CT); control tumour + botryosphaeran (CTB); obese tumour (OT), and obese tumour + botryosphaeran (OTB). In ninth week, obese and non-obese rats were inoculated with 1 × 107 Walker-256 tumour cells and treated with botryosphaeran (30 mg/kg/d for 15 days). In 11th week, the following parameters were evaluated glycogen, glucose and lactate levels, pro-oxidant (TBARS) and antioxidant markers (superoxide dismutase [SOD]; catalase [CAT]; glutathione-S-transferase [GST]; reduced glutathione [GSH]; vitamin C) and cytokines. Obesity presented oxidative stress and inflammation, as demonstrated by high levels of TBARS, SOD and TNF-α, and lower levels of CAT, GSH and interleukin-10 (IL-10). Botryosphaeran significantly decreased TBARS and TNF-α and increased GST, GSH, vitamin C and IL-10 in the liver; increased SOD and vitamin C in tumour tissue; decreased TBARS in adipose tissue, and notably decreased the levels of glycogen and lactate in the tumour of CTB rats. Botryosphaeran promoted significant antioxidant, anti-inflammatory, and beneficial metabolic effects in Walker-256 tumour-bearing non-obese and obese rats, which contributed to its antitumour activity.
cancer, β-Glucans, Botryosphaeria rhodina MAMB-05, obesity, oxidative damage
Structure type: fragment of a bigger structure
Location inside paper: abstract, p. 214
Trivial name: β-1,3-glucan, botryosphaeran, β-glucan
Compound class: EPS, cell wall polysaccharide, β-gucan
Contained glycoepitopes: IEDB_1397514,IEDB_141806,IEDB_142488,IEDB_146664,IEDB_153543,IEDB_158555,IEDB_161166,IEDB_241101,IEDB_558869,IEDB_857743,IEDB_983931,SB_192
Methods: ELISA, biochemical methods, statistical analysis, cytokine production, histological analysis, animals and experimental protocol
Biological activity: Botryosphaeran presented significant antioxidant, anti-inflammatory, and metabolic effects in both, the obese and non-obese rat groups bearing Walker-256 tumour. The fungal β-glucan acted by protecting liver and adipose tissue from damage induced by ROS/RNS, obesity, and the tumour itself, thus reducing tumour development.
NCBI Taxonomy refs (TaxIDs): 45133Reference(s) to other database(s): GTC:G83138WS
Show glycosyltransferases
There is only one chemically distinct structure:
Taxonomic group: fungi / Basidiomycota
(Phylum: Basidiomycota)
Organ / tissue: fruiting bodies
NCBI PubMed ID: 35571333Publication DOI: 10.1016/j.fochx.2022.100321Journal NLM ID: 101751436Publisher: Elsevier
Correspondence: Y. Xie <xieyz
gdim.cn>; Q. Wu <wuqp203
163.com>
Institutions: Department of Food Science, Rutgers University, New Brunswick, NJ, USA, College of Food Science, South China Agricultural University, 483 Wushan Street, Tianhe District, Guangzhou, China, State Key Laboratory of Applied Microbiology Southern China, Guangdong Provincial Key Laboratory of Microbial Safety and Health, Key Laboratory of Agricultural Microbiomics and Precision Application, Ministry of Agriculture and Rural Affairs, Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou 510070, China
The chemical structure of GLP-1, a novel water-soluble heteropolysaccharide purified Ganoderma leucocontextum fruiting bodies, has been characterized in our previous study. This study aimed to investigate the immunostimulatory activity of GLP-1 in vitro and in vivo by using RAW264.7 macrophages and cyclophosphamide-induced immunosuppressed mice model. Results showed that GLP-1 was able to enhance phagocytic activity and promote the production of reactive oxygen species, nitric oxide, tumor necrosis factor-?, interleukin-6, and monocyte chemoattractant protein-1 in RAW264.7 macrophages. Moreover, GLP-1 could activate mitogen-activated protein kinase, phosphatidylinositol-3-kinase/protein kinase B, and nuclear factor-kappa B signaling pathways through toll-like receptor 2 and dectin-1 receptors. Furthermore, GLP-1 increased the thymus index, serum immunoglobulin levels, and percentage of CD3+ T lymphocytes in cyclophosphamide-induced immunosuppressed mice. These results demonstrated that GLP-1 possessed significant immunostimulatory effects in vivo and in vitro and could be developed as an effective immunomodulator for application in functional foods.
heteropolysaccharide, Immunostimulatory activity, Ganoderma leucocontextum, immunosuppressed mice, molecular mechanism
Structure type: fragment of a bigger structure ; 159700
Location inside paper: p. 100321-8
Trivial name: water-soluble heteropolysaccharide GLP-3
Contained glycoepitopes: IEDB_140629,IEDB_142488,IEDB_144998,IEDB_146664,IEDB_420417,IEDB_420418,IEDB_420421,IEDB_857742,IEDB_983931,SB_192
Methods: Western blotting, cytokine analysis, extraction, RT-PCR, statistical analysis, cell viability assay, phagocytosis assay, ROS measurement, animals and experimental protocol
Comments, role: the backbone of GLP-3
NCBI Taxonomy refs (TaxIDs): 1566825
Show glycosyltransferases
There is only one chemically distinct structure:
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(later renamed to: Lasiodiplodia theobromae MAMB-05)