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Tang N, Wang X, Yang R, Liu Z, Liu Y, Tian J, Xiao L, Li W
Extraction, isolation, structural characterization and prebiotic activity of cell wall polysaccharide from Kluyveromyces marxianus
Carbohydrate Polymers 289 (2022)
119457
|
a-D-Manp-(1-2)-+
|
a-D-Manp-(1-2)-+ |
| |
a-D-Manp-(1-2)-+ | |
| | |
a-D-Manp-(1-2)-a-D-Manp-(1-2)-+ | | |
| | | |
-6)-a-D-Manp-(1-6)-a-D-Manp-(1-6)-a-D-Manp-(1-6)-a-D-Manp-(1-6)-a-D-Manp-(1- |
Show graphically |
Kluyveromyces marxianus LZ-JM-1
(Ancestor NCBI TaxID 4911,
species name lookup)
Taxonomic group: fungi / Ascomycota
(Phylum: Ascomycota)
Organ / tissue: cell wall
The structure was elucidated in this paperPublication DOI: 10.1016/j.carbpol.2022.119457Journal NLM ID: 8307156Publisher: Elsevier
Correspondence: W. Li <lw1981
njau.edu.cn>
Institutions: College of Food Science and Technology, Nanjing Agricultural University, Nanjing, Jiangsu 210095, PR China
In this study, three yeast α-mannans (LZ-MPS, MC-MPS, and G-MPS) were extracted from different sources of Kluyveromyces marxianus. The total sugar content of the three α-mannans ranged from 91.13–97.10%, whereas no proteins were detected. A structural arrangement was proposed using ultraviolet spectroscopy, Fourier-transform infrared spectroscopy, and one-dimensional and two-dimensional nuclear magnetic resonance. The main chain of the three yeast α-mannans was formed by a →6)-α-D-Manp-(1→ unit, which was slightly different from the repeating unit of the branch structure. The prebiotic potential of LZ-MPS, MC-MPS, and G-MPS was assessed using in vitro fermentation with pure and faecal cultures. The three yeast α-mannans could be utilised as substrates for the growth of Lactobacillus and Lactococcus strains. In addition, the three yeast α-mannans markedly regulated the intestinal microbiota composition by increasing the relative abundances of Bacteroides, Parabacteroides, and Phascolarctobacterium and decreasing the abundance of pathogenic bacteria.
Lactobacillus, Structural characterization, gut microbiota, in vitro fermentation, prebiotic activity, Kluyveromyces marxianus, yeast cell wall polysaccharide
Structure type: polymer chemical repeating unit
Location inside paper: Fig. 4(a), LZ-MPS
Trivial name: α-mannan
Compound class: cell wall polysaccharide
Contained glycoepitopes: IEDB_130701,IEDB_136104,IEDB_140116,IEDB_141793,IEDB_141828,IEDB_141829,IEDB_141830,IEDB_141831,IEDB_141832,IEDB_143632,IEDB_144983,IEDB_152206,IEDB_153220,IEDB_153762,IEDB_153763,IEDB_76933,IEDB_857732,IEDB_857735,IEDB_983930,SB_136,SB_191,SB_196,SB_198,SB_44,SB_67,SB_72
Methods: 13C NMR, 1H NMR, NMR-2D, sugar analysis, anion-exchange chromatography, FTIR, HPLC, HPSEC, UV, extraction, statistical analysis, analysis of gut microbiota, In vitro fermentation
Comments, role: Kluyveromyces marxianus LZ-JM-1 was isolated from Tibetan kefir
Related record ID(s): 51835, 51836
NCBI Taxonomy refs (TaxIDs): 4911
Show glycosyltransferases
NMR conditions: in D2O at 313 K
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6
6,6,6,6,2,2 aDManp 102.08 70.08 70.58 66.83 73.46 61.08
6,6,6,6,2 aDManp 100.71 78.46 70.58 65.83 73.46 61.08
6,6,6,6 aDManp 98.33 78.71 70.58 66.58 73.46 65.71
6,6,6,2 aDManp 102.08 70.08 70.58 66.83 73.46 61.08
6,6,6 aDManp 98.33 78.71 70.58 66.58 73.46 65.71
6,6,2 aDManp 102.08 70.08 70.58 66.83 73.46 61.08
6,6 aDManp 98.33 78.71 70.58 66.58 73.46 65.71
6,2 aDManp 102.08 70.08 70.58 66.83 73.46 61.08
6 aDManp 98.33 78.71 70.58 66.58 73.46 65.71
aDManp 98.33 70.43 70.58 66.58 73.46 65.71
1H NMR data:
Linkage Residue H1 H2 H3 H4 H5 H6
6,6,6,6,2,2 aDManp 5.05 4.08 3.83 3.68 3.77 3.76-3.89
6,6,6,6,2 aDManp 5.29 4.12 3.92 3.70 3.77 3.76-3.89
6,6,6,6 aDManp 5.12 4.04 3.92 3.81 3.76 4.01
6,6,6,2 aDManp 5.05 4.08 3.83 3.68 3.77 3.76-3.89
6,6,6 aDManp 5.12 4.04 3.92 3.81 3.76 4.01
6,6,2 aDManp 5.05 4.08 3.83 3.68 3.77 3.76-3.89
6,6 aDManp 5.12 4.04 3.92 3.81 3.76 4.01
6,2 aDManp 5.05 4.08 3.83 3.68 3.77 3.76-3.89
6 aDManp 5.12 4.04 3.92 3.81 3.76 4.01
aDManp 5.09 4.06 3.92 3.81 3.76 4.01
1H/13C HSQC data:
Linkage Residue C1/H1 C2/H2 C3/H3 C4/H4 C5/H5 C6/H6
6,6,6,6,2,2 aDManp 102.08/5.05 70.08/4.08 70.58/3.83 66.83/3.68 73.46/3.77 61.08/3.76-3.89
6,6,6,6,2 aDManp 100.71/5.29 78.46/4.12 70.58/3.92 65.83/3.70 73.46/3.77 61.08/3.76-3.89
6,6,6,6 aDManp 98.33/5.12 78.71/4.04 70.58/3.92 66.58/3.81 73.46/3.76 65.71/4.01
6,6,6,2 aDManp 102.08/5.05 70.08/4.08 70.58/3.83 66.83/3.68 73.46/3.77 61.08/3.76-3.89
6,6,6 aDManp 98.33/5.12 78.71/4.04 70.58/3.92 66.58/3.81 73.46/3.76 65.71/4.01
6,6,2 aDManp 102.08/5.05 70.08/4.08 70.58/3.83 66.83/3.68 73.46/3.77 61.08/3.76-3.89
6,6 aDManp 98.33/5.12 78.71/4.04 70.58/3.92 66.58/3.81 73.46/3.76 65.71/4.01
6,2 aDManp 102.08/5.05 70.08/4.08 70.58/3.83 66.83/3.68 73.46/3.77 61.08/3.76-3.89
6 aDManp 98.33/5.12 78.71/4.04 70.58/3.92 66.58/3.81 73.46/3.76 65.71/4.01
aDManp 98.33/5.09 70.43/4.06 70.58/3.92 66.58/3.81 73.46/3.76 65.71/4.01
1H NMR data:
| Linkage | Residue | H1 | H2 | H3 | H4 | H5 | H6 |
|---|
| 6,6,6,6,2,2 | aDManp | 5.05 | 4.08 | 3.83 | 3.68 | 3.77 | 3.76
3.89 |
| 6,6,6,6,2 | aDManp | 5.29 | 4.12 | 3.92 | 3.70 | 3.77 | 3.76
3.89 |
| 6,6,6,6 | aDManp | 5.12 | 4.04 | 3.92 | 3.81 | 3.76 | 4.01 |
| 6,6,6,2 | aDManp | 5.05 | 4.08 | 3.83 | 3.68 | 3.77 | 3.76
3.89 |
| 6,6,6 | aDManp | 5.12 | 4.04 | 3.92 | 3.81 | 3.76 | 4.01 |
| 6,6,2 | aDManp | 5.05 | 4.08 | 3.83 | 3.68 | 3.77 | 3.76
3.89 |
| 6,6 | aDManp | 5.12 | 4.04 | 3.92 | 3.81 | 3.76 | 4.01 |
| 6,2 | aDManp | 5.05 | 4.08 | 3.83 | 3.68 | 3.77 | 3.76
3.89 |
| 6 | aDManp | 5.12 | 4.04 | 3.92 | 3.81 | 3.76 | 4.01 |
| | aDManp | 5.09 | 4.06 | 3.92 | 3.81 | 3.76 | 4.01 |
|
13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 |
|---|
| 6,6,6,6,2,2 | aDManp | 102.08 | 70.08 | 70.58 | 66.83 | 73.46 | 61.08 |
| 6,6,6,6,2 | aDManp | 100.71 | 78.46 | 70.58 | 65.83 | 73.46 | 61.08 |
| 6,6,6,6 | aDManp | 98.33 | 78.71 | 70.58 | 66.58 | 73.46 | 65.71 |
| 6,6,6,2 | aDManp | 102.08 | 70.08 | 70.58 | 66.83 | 73.46 | 61.08 |
| 6,6,6 | aDManp | 98.33 | 78.71 | 70.58 | 66.58 | 73.46 | 65.71 |
| 6,6,2 | aDManp | 102.08 | 70.08 | 70.58 | 66.83 | 73.46 | 61.08 |
| 6,6 | aDManp | 98.33 | 78.71 | 70.58 | 66.58 | 73.46 | 65.71 |
| 6,2 | aDManp | 102.08 | 70.08 | 70.58 | 66.83 | 73.46 | 61.08 |
| 6 | aDManp | 98.33 | 78.71 | 70.58 | 66.58 | 73.46 | 65.71 |
| | aDManp | 98.33 | 70.43 | 70.58 | 66.58 | 73.46 | 65.71 |
|
There is only one chemically distinct structure:
Expand this record
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Tang N, Wang X, Yang R, Liu Z, Liu Y, Tian J, Xiao L, Li W
Extraction, isolation, structural characterization and prebiotic activity of cell wall polysaccharide from Kluyveromyces marxianus
Carbohydrate Polymers 289 (2022)
119457
Kluyveromyces marxianus MC-S-E
(Ancestor NCBI TaxID 4911,
species name lookup)
Taxonomic group: fungi / Ascomycota
(Phylum: Ascomycota)
Organ / tissue: cell wall
The structure was elucidated in this paperPublication DOI: 10.1016/j.carbpol.2022.119457Journal NLM ID: 8307156Publisher: Elsevier
Correspondence: W. Li <lw1981
njau.edu.cn>
Institutions: College of Food Science and Technology, Nanjing Agricultural University, Nanjing, Jiangsu 210095, PR China
In this study, three yeast α-mannans (LZ-MPS, MC-MPS, and G-MPS) were extracted from different sources of Kluyveromyces marxianus. The total sugar content of the three α-mannans ranged from 91.13–97.10%, whereas no proteins were detected. A structural arrangement was proposed using ultraviolet spectroscopy, Fourier-transform infrared spectroscopy, and one-dimensional and two-dimensional nuclear magnetic resonance. The main chain of the three yeast α-mannans was formed by a →6)-α-D-Manp-(1→ unit, which was slightly different from the repeating unit of the branch structure. The prebiotic potential of LZ-MPS, MC-MPS, and G-MPS was assessed using in vitro fermentation with pure and faecal cultures. The three yeast α-mannans could be utilised as substrates for the growth of Lactobacillus and Lactococcus strains. In addition, the three yeast α-mannans markedly regulated the intestinal microbiota composition by increasing the relative abundances of Bacteroides, Parabacteroides, and Phascolarctobacterium and decreasing the abundance of pathogenic bacteria.
Lactobacillus, Structural characterization, gut microbiota, in vitro fermentation, prebiotic activity, Kluyveromyces marxianus, yeast cell wall polysaccharide
Structure type: polymer chemical repeating unit
Location inside paper: Fig. 4(b), MC-MPS
Trivial name: mannan, neutral mannan, BCG-PSN (Bacillus Calmette-Guérin polysaccharide and nucleic acid), α-mannan
Compound class: cell wall polysaccharide
Contained glycoepitopes: IEDB_130701,IEDB_136104,IEDB_140116,IEDB_141793,IEDB_141828,IEDB_141829,IEDB_141831,IEDB_143632,IEDB_144983,IEDB_152206,IEDB_153220,IEDB_153762,IEDB_153763,IEDB_76933,IEDB_857732,IEDB_857735,IEDB_983930,SB_136,SB_191,SB_196,SB_198,SB_44,SB_67,SB_72
Methods: 13C NMR, 1H NMR, NMR-2D, sugar analysis, anion-exchange chromatography, FTIR, HPLC, HPSEC, UV, extraction, statistical analysis, analysis of gut microbiota, In vitro fermentation
Comments, role: Kluyveromyces marxianus MC-S-E was isolated from traditional Xinjiang fermented dairy products
Related record ID(s): 51662, 51836
NCBI Taxonomy refs (TaxIDs): 4911Reference(s) to other database(s): GTC:G75213RY, GlycomeDB:
16270, CCSD:
4940, CBank-STR:4613
Show glycosyltransferases
NMR conditions: in D2O at 313 K
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6
2 aDManp 102.05 70.12 70.56 66.45 73.49 61.18
aDManp 98.10 78.76 70.41 66.60 73.49 65.72
1H NMR data:
Linkage Residue H1 H2 H3 H4 H5 H6
2 aDManp 5.10 4.13 3.88 3.73 3.82 3.80-3.93
aDManp 5.16 4.09 3.97 3.86 3.80 4.07
1H/13C HSQC data:
Linkage Residue C1/H1 C2/H2 C3/H3 C4/H4 C5/H5 C6/H6
2 aDManp 102.05/5.10 70.12/4.13 70.56/3.88 66.45/3.73 73.49/3.82 61.18/3.80-3.93
aDManp 98.10/5.16 78.76/4.09 70.41/3.97 66.60/3.86 73.49/3.80 65.72/4.07
1H NMR data:
| Linkage | Residue | H1 | H2 | H3 | H4 | H5 | H6 |
|---|
| 2 | aDManp | 5.10 | 4.13 | 3.88 | 3.73 | 3.82 | 3.80
3.93 |
| | aDManp | 5.16 | 4.09 | 3.97 | 3.86 | 3.80 | 4.07 |
|
13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 |
|---|
| 2 | aDManp | 102.05 | 70.12 | 70.56 | 66.45 | 73.49 | 61.18 |
| | aDManp | 98.10 | 78.76 | 70.41 | 66.60 | 73.49 | 65.72 |
|
There is only one chemically distinct structure:
Expand this record
Collapse this record
Tang N, Wang X, Yang R, Liu Z, Liu Y, Tian J, Xiao L, Li W
Extraction, isolation, structural characterization and prebiotic activity of cell wall polysaccharide from Kluyveromyces marxianus
Carbohydrate Polymers 289 (2022)
119457
|
a-D-Manp-(1-2)-+
|
a-D-Manp-(1-2)-+ |
| |
a-D-Manp-(1-2)-a-D-Manp-(1-2)-+ | |
| | |
-6)-a-D-Manp-(1-6)-a-D-Manp-(1-6)-a-D-Manp-(1-6)-a-D-Manp-(1- |
Show graphically |
Kluyveromyces marxianus G-Y3
(Ancestor NCBI TaxID 4911,
species name lookup)
Taxonomic group: fungi / Ascomycota
(Phylum: Ascomycota)
Organ / tissue: cell wall
The structure was elucidated in this paperPublication DOI: 10.1016/j.carbpol.2022.119457Journal NLM ID: 8307156Publisher: Elsevier
Correspondence: W. Li <lw1981
njau.edu.cn>
Institutions: College of Food Science and Technology, Nanjing Agricultural University, Nanjing, Jiangsu 210095, PR China
In this study, three yeast α-mannans (LZ-MPS, MC-MPS, and G-MPS) were extracted from different sources of Kluyveromyces marxianus. The total sugar content of the three α-mannans ranged from 91.13–97.10%, whereas no proteins were detected. A structural arrangement was proposed using ultraviolet spectroscopy, Fourier-transform infrared spectroscopy, and one-dimensional and two-dimensional nuclear magnetic resonance. The main chain of the three yeast α-mannans was formed by a →6)-α-D-Manp-(1→ unit, which was slightly different from the repeating unit of the branch structure. The prebiotic potential of LZ-MPS, MC-MPS, and G-MPS was assessed using in vitro fermentation with pure and faecal cultures. The three yeast α-mannans could be utilised as substrates for the growth of Lactobacillus and Lactococcus strains. In addition, the three yeast α-mannans markedly regulated the intestinal microbiota composition by increasing the relative abundances of Bacteroides, Parabacteroides, and Phascolarctobacterium and decreasing the abundance of pathogenic bacteria.
Lactobacillus, Structural characterization, gut microbiota, in vitro fermentation, prebiotic activity, Kluyveromyces marxianus, yeast cell wall polysaccharide
Structure type: polymer chemical repeating unit
Location inside paper: Fig. 4(c), G-MPS
Trivial name: α-mannan
Compound class: cell wall polysaccharide
Contained glycoepitopes: IEDB_130701,IEDB_136104,IEDB_140116,IEDB_141793,IEDB_141828,IEDB_141829,IEDB_141830,IEDB_141831,IEDB_141832,IEDB_143632,IEDB_144983,IEDB_152206,IEDB_153220,IEDB_153762,IEDB_153763,IEDB_76933,IEDB_857732,IEDB_857735,IEDB_983930,SB_136,SB_191,SB_196,SB_198,SB_44,SB_67,SB_72
Methods: 13C NMR, 1H NMR, NMR-2D, sugar analysis, anion-exchange chromatography, FTIR, HPLC, HPSEC, UV, extraction, statistical analysis, analysis of gut microbiota, In vitro fermentation
Comments, role: Kluyveromyces marxianus G-Y3 was isolated from Tibetan kefir
Related record ID(s): 51662, 51835
NCBI Taxonomy refs (TaxIDs): 4911
Show glycosyltransferases
NMR conditions: in D2O at 313 K
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6
6,6,6,2,2 aDManp 102.64 70.41 70.15 66.90 73.34 61.62
6,6,6,2 aDManp 100.88 78.62 71.00 66.31 73.34 61.62
6,6,6 aDManp 98.54 78.91 71.00 66.90 73.34 65.73
6,6,2 aDManp 102.64 70.41 70.15 66.90 73.34 61.62
6,6 aDManp 98.54 78.91 71.00 66.90 73.34 65.73
6,2 aDManp 102.64 70.41 70.15 66.90 73.34 61.62
6 aDManp 98.54 78.91 71.00 66.90 73.34 65.73
aDManp 98.54 70.45 71.00 66.90 73.34 65.73
1H NMR data:
Linkage Residue H1 H2 H3 H4 H5 H6
6,6,6,2,2 aDManp 5.07 4.09 3.84 3.70 3.78 3.77-3.90
6,6,6,2 aDManp 5.30 4.13 3.93 3.71 3.78 3.77-3.90
6,6,6 aDManp 5.13 4.05 3.97 3.82 3.77 4.03
6,6,2 aDManp 5.07 4.09 3.84 3.70 3.78 3.77-3.90
6,6 aDManp 5.13 4.05 3.97 3.82 3.77 4.03
6,2 aDManp 5.07 4.09 3.84 3.70 3.78 3.77-3.90
6 aDManp 5.13 4.05 3.97 3.82 3.77 4.03
aDManp 5.10 4.07 3.97 3.82 3.77 4.03
1H/13C HSQC data:
Linkage Residue C1/H1 C2/H2 C3/H3 C4/H4 C5/H5 C6/H6
6,6,6,2,2 aDManp 102.64/5.07 70.41/4.09 70.15/3.84 66.90/3.70 73.34/3.78 61.62/3.77-3.90
6,6,6,2 aDManp 100.88/5.30 78.62/4.13 71.00/3.93 66.31/3.71 73.34/3.78 61.62/3.77-3.90
6,6,6 aDManp 98.54/5.13 78.91/4.05 71.00/3.97 66.90/3.82 73.34/3.77 65.73/4.03
6,6,2 aDManp 102.64/5.07 70.41/4.09 70.15/3.84 66.90/3.70 73.34/3.78 61.62/3.77-3.90
6,6 aDManp 98.54/5.13 78.91/4.05 71.00/3.97 66.90/3.82 73.34/3.77 65.73/4.03
6,2 aDManp 102.64/5.07 70.41/4.09 70.15/3.84 66.90/3.70 73.34/3.78 61.62/3.77-3.90
6 aDManp 98.54/5.13 78.91/4.05 71.00/3.97 66.90/3.82 73.34/3.77 65.73/4.03
aDManp 98.54/5.10 70.45/4.07 71.00/3.97 66.90/3.82 73.34/3.77 65.73/4.03
1H NMR data:
| Linkage | Residue | H1 | H2 | H3 | H4 | H5 | H6 |
|---|
| 6,6,6,2,2 | aDManp | 5.07 | 4.09 | 3.84 | 3.70 | 3.78 | 3.77
3.90 |
| 6,6,6,2 | aDManp | 5.30 | 4.13 | 3.93 | 3.71 | 3.78 | 3.77
3.90 |
| 6,6,6 | aDManp | 5.13 | 4.05 | 3.97 | 3.82 | 3.77 | 4.03 |
| 6,6,2 | aDManp | 5.07 | 4.09 | 3.84 | 3.70 | 3.78 | 3.77
3.90 |
| 6,6 | aDManp | 5.13 | 4.05 | 3.97 | 3.82 | 3.77 | 4.03 |
| 6,2 | aDManp | 5.07 | 4.09 | 3.84 | 3.70 | 3.78 | 3.77
3.90 |
| 6 | aDManp | 5.13 | 4.05 | 3.97 | 3.82 | 3.77 | 4.03 |
| | aDManp | 5.10 | 4.07 | 3.97 | 3.82 | 3.77 | 4.03 |
|
13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 |
|---|
| 6,6,6,2,2 | aDManp | 102.64 | 70.41 | 70.15 | 66.90 | 73.34 | 61.62 |
| 6,6,6,2 | aDManp | 100.88 | 78.62 | 71.00 | 66.31 | 73.34 | 61.62 |
| 6,6,6 | aDManp | 98.54 | 78.91 | 71.00 | 66.90 | 73.34 | 65.73 |
| 6,6,2 | aDManp | 102.64 | 70.41 | 70.15 | 66.90 | 73.34 | 61.62 |
| 6,6 | aDManp | 98.54 | 78.91 | 71.00 | 66.90 | 73.34 | 65.73 |
| 6,2 | aDManp | 102.64 | 70.41 | 70.15 | 66.90 | 73.34 | 61.62 |
| 6 | aDManp | 98.54 | 78.91 | 71.00 | 66.90 | 73.34 | 65.73 |
| | aDManp | 98.54 | 70.45 | 71.00 | 66.90 | 73.34 | 65.73 |
|
There is only one chemically distinct structure:
Expand this record
Collapse this record
Total list of record IDs on all result pages of the current query:
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